Dual-chambered bio-batteries using immobilized mediator electrodes
Methylene blue was immobilized on 304L stainless steel to investigate a potential cost-effective, durable, and high performance composite electrode for use with microbial applications, such as bio-batteries and microbial fuel cells. The composite electrodes were tested in dual-chamber bio-batteries with pure cultures of Escherichia coli K-12 or Shewanella oneidensis MR-1 and the results were compared to those obtained using bare graphite electrodes. The maximum power generated using the composite electrodes was 39.35 mW m−2 in bio-batteries using E. coli K-12, and 60.05 mW m−2 in bio-batteries using S. oneidensis MR-1. Compared to graphite electrodes, the bio-batteries using composite electrodes showed a 6- and 2.5-fold increase in the maximum power density, using pure cultures of E. coli K-12 and S. oneidensis MR-1, respectively. The composite electrodes did not inhibit bacterial growth in the bio-batteries and were shown to improve performance (both in terms of power output and current density) over conventional graphite electrodes.
KeywordsBio-battery Immobilized mediator electrodes Stainless steel electrodes Polarization Microbial
The authors gratefully acknowledge financial support from the Natural Science and Engineering Research Council of Canada (NSERC).
- 2.Zhang Y, Sun J, Hu Y, Li S, Xu Q (2012) Bio-cathode materials evaluation in microbial fuel cells: a comparison of graphite felt, carbon paper and stainless steel mesh materials. Intern J Hydrog Energy 37:16935–16942Google Scholar
- 6.Godwin J, Evitts R, Kennell G (2012) Microbial fuel cell with a polypyrrole/poly(methylene blue) composite electrode. Rep Electrochem. 2:3–11Google Scholar
- 13.Dávila D, Esquivel JP, Vigués N, Sánchez O, Garrido L, Tomás N, Sabaté N, Campo FJ, Muñoz FJ, Mas J (2008) Development and optimization of microbial fuel cells. J New Mater Electrochem Sys 11:99–103Google Scholar
- 15.Godwin, Jonathan (2011) Immobilized mediator electrodes for biocathode microbial fuel cells. Dissertation, University of SaskatchewanGoogle Scholar
- 17.Gorby YA, Yanina S, McLean SJ, Rosso MK, Moyles D, Dohnalkova A, Beveridge JT, Chang SI, Kim BH, Kim SK, Culley ED, Reed BS, Romine FM, Sarrarini AD, Hill AE, Shi L, Elias AD, Kennedy WD, Pinchuk G, Watanabe K, Ishii S, Logan B, Nealson HK, Fredrickson KJ (2006) Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms. Nat Acad Sci. 103(30):11358–11363CrossRefGoogle Scholar