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Development of glucose oxidase-based bioanodes for enzyme fuel cell applications

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Abstract

We fabricated an enzyme fuel cell (EFC) device based on glucose as fuel and glucose oxidase (GOx) as biocatalyst. As a strategy to improve GOx stability, preserving at the same time the enzyme catalytic activity, we propose an immobilization procedure to entrap GOx in a polymer matrix based on Nafion and multiwalled carbon nanotubes. Circular dichroism (CD) spectra were recorded to study changes in the 3D structure of GOx that might be generated by the immobilization procedure. The comparison between the CD features of GOx immobilized and free in solution indicates that the shape of the spectra and position of peaks do not significantly change. The bioelectrocatalytic activity toward glucose oxidation of immobilized GOx was studied by cyclic voltammetry and chronoamperometry experiments. Such electrochemical experiments allow monitoring the rate of GOx-catalyzed glucose oxidation and extrapolating GOx kinetic parameters. Results demonstrate that immobilized GOx has high catalytic efficiency, due the maintaining of regular and well-ordered structure of the immobilized enzyme, as indicated by spectroscopic findings. Once investigated the electrode structure–property relationship, an EFC device was assembled using the GOx-based bioanode, and sulfonated poly ether ether ketone as electrolyte membrane. Polarization and power density curves of the complete EFC device were acquired, demonstrating the suitability of the immobilization strategy and materials to be used in EFCs.

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Acknowledgments

The financial support of the Italian Ministry for Environment (MATTM, Project MECH2), the Ager Consortium and Fapesp/CNPq (Brazilian Funding Agencies) is gratefully acknowledged. Thanks are due to Professor Nicola Rosato (Department of Experimental Medicine and Biochemical Sciences & NAST Center, University of Rome Tor Vergata) for help in collecting CD data.

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Authors and Affiliations

  1. Department of Chemical Science and Technology & NAST Centre, University of Rome “Tor Vergata”, Rome, Italy

    Barbara Mecheri, Alessandra D’Epifanio, Antonio Geracitano & Silvia Licoccia

  2. Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, São Paulo, Brazil

    Patricia Targon Campana

Authors
  1. Barbara Mecheri
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  2. Alessandra D’Epifanio
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  3. Antonio Geracitano
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  4. Patricia Targon Campana
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  5. Silvia Licoccia
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Correspondence to Barbara Mecheri or Silvia Licoccia.

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Mecheri, B., D’Epifanio, A., Geracitano, A. et al. Development of glucose oxidase-based bioanodes for enzyme fuel cell applications. J Appl Electrochem 43, 181–190 (2013). https://doi.org/10.1007/s10800-012-0489-y

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  • DOI: https://doi.org/10.1007/s10800-012-0489-y

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