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Characterization of poly(vinylferrocenium) coated surfaces and their applications in DNA sensor technology

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Abstract

Poly(vinylferrocenium) (PVF+) modified gold (Au) electrode was developed in this study for the electrochemical sensing of deoxyribonucleic acid (DNA) hybridization based on the oxidation signals of polymer and guanine, and also for the electrochemical investigation of interaction of anticancer drug, mitomycin C (MC) and DNA immobilized onto PVF+ modified Au electrode. PVF+ modified Au electrode was prepared by electrooxidation of poly(vinylferrocene) PVF at +0.7 V versus Ag/AgCl reference electrode. The polymer modified electrode and DNA immobilized polymer modified electrode were characterized by X-ray photoelectron (XPS), Fourier transform infrared-attenuated total reflentance (FTIR-ATR) and alternating current (AC) impedance spectroscopy. For application studies, differential pulse voltammetry (DPV) technique was used.

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Acknowledgments

This study was supported by Turkish Scientific and Technological Council (TUBITAK) (Project no: 106T065). A.E. expresses her gratitudes to the Turkish Academy of Sciences (TUBA) as an Associate member for their support.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Hacettepe University, 06532, Beytepe, Ankara, Turkey

    Filiz Kuralay, Serdar Abacı, Haluk Özyörük & Attila Yıldız

  2. Analytical Chemistry Department, Faculty of Pharmacy, Ege University, 35100, Bornova, Izmir, Turkey

    Arzum Erdem

Authors
  1. Filiz Kuralay
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  2. Arzum Erdem
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  3. Serdar Abacı
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  4. Haluk Özyörük
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  5. Attila Yıldız
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Correspondence to Filiz Kuralay or Arzum Erdem.

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Kuralay, F., Erdem, A., Abacı, S. et al. Characterization of poly(vinylferrocenium) coated surfaces and their applications in DNA sensor technology. J Appl Electrochem 40, 2039–2050 (2010). https://doi.org/10.1007/s10800-010-0185-8

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  • DOI: https://doi.org/10.1007/s10800-010-0185-8

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