Journal of Applied Electrochemistry

, Volume 39, Issue 7, pp 971–977 | Cite as

Development of a new biosensor for mediatorless voltammetric determination of hydrogen peroxide and its application in milk samples

  • Şenol AlpatEmail author
  • Sibel Kılınç Alpat
  • Zekerya Dursun
  • Azmi Telefoncu
Original Paper


A new biosensor for the voltammetric detection of hydrogen peroxide was developed based on immobilization of catalase on a clinoptilolite modified carbon paste electrode using bovine serum albumin and glutaraldehyde. The biosensor response was evaluated according to electrode composition, reaction time, solution pH and temperature. The voltammetric signals were linearly in proportion to H2O2 concentration in the range 5.0 × 10−6–1.0 × 10−3 M with a correlation coefficient of 0.9975. The detection limit is 8.0 × 10−7 M and the relative standard deviation for 4.0 × 10−4 M hydrogen peroxide was 1.83% (= 6). The biosensor exhibited high sensitivity, and it was determined that it could be used for more than 2 months. In addition, the biosensor was successfully applied for the determination of hydrogen peroxide in milk samples.


Hydrogen peroxide Voltammetry Biosensor Clinoptilolite Catalase Milk 



The authors express their thankfulness to Associate Prof. Dr. Stephen T. Astley, Department of Chemistry, Faculty of Science, Ege University, for his careful review of the manuscript.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Şenol Alpat
    • 1
    Email author
  • Sibel Kılınç Alpat
    • 1
  • Zekerya Dursun
    • 2
  • Azmi Telefoncu
    • 3
  1. 1.Department of Chemistry EducationDokuz Eylül UniversityBuca-IzmirTurkey
  2. 2.Department of ChemistryEge UniversityBornova-IzmirTurkey
  3. 3.Department of BiochemistryEge UniversityBornova-IzmirTurkey

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