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Development of an amperometric sensor for simultaneous determination of uric acid and ascorbic acid using 2-[bis(2-aminoethyl)amino]ethanol, 4,4′-bipyridine bridged dicopper(II) complex

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Abstract

Binuclear copper complex (2-[bis(2-aminoethyl)amino]ethanol, 4,4′-bipyridine bridged dicopper(II) complex) was grafted onto the surface of a glassy carbon electrode (GCE) using the cyclic voltammetric method in a phosphate buffer solution (PBS). The modified electrode resulted in efficient electrocatalytic activity for anodic oxidation of uric acid (UA) and ascorbic acid (AA) via a substantial decrease in anodic over-potentials for both compounds. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) using this modified electrode result in two well-resolved anodic waves for the oxidation of UA and AA in mixed solution, making possible the simultaneous determination of both compounds. Linear analytical curves were obtained in the ranges 5.0–300.0 μM and 5.0–160.0 μM for UA and AA concentrations through DPV methods, respectively. The detection limits were 2.0 μM of UA and AA. This electrode was used for UA and AA determinations in urine samples with satisfactory results.

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Acknowledgements

We gratefully acknowledge financial support from the Natural Research Fundation of JiangSu Province (BK2005045) and the Natural Research Fundation of Huaihai Institute of Technology (2008HGXK002).

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Authors and Affiliations

  1. Department of Chemical Engineering, Huaihai Institute of Technology, Lianyungang, Jiangsu Province, 222005, China

    Ming Yan Wang, Xing You Xu, Fan Yang & Sheng Yu Zhang

  2. Laboratory of Materials Chemistry, Nanjing University of Science and Technology, Nanjing, 210094, China

    Ming Yan Wang, Xing You Xu & Xu Jie Yang

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  1. Ming Yan Wang
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  2. Xing You Xu
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  3. Fan Yang
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  4. Sheng Yu Zhang
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  5. Xu Jie Yang
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Correspondence to Xing You Xu.

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Wang, M.Y., Xu, X.Y., Yang, F. et al. Development of an amperometric sensor for simultaneous determination of uric acid and ascorbic acid using 2-[bis(2-aminoethyl)amino]ethanol, 4,4′-bipyridine bridged dicopper(II) complex. J Appl Electrochem 38, 1269–1274 (2008). https://doi.org/10.1007/s10800-008-9552-0

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  • DOI: https://doi.org/10.1007/s10800-008-9552-0

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