Journal of Applied Electrochemistry

, Volume 37, Issue 8, pp 893–898 | Cite as

Rapid determination of triazophos using acetylcholinesterase biosensor based on sol–gel interface assembling multiwall carbon nanotubes

  • Dan DuEmail author
  • Jie Cai
  • Dandan Song
  • Aidong Zhang
Original Paper


A sensitive and stable amperometric sensor has been devised for rapid determination of triazophos based on efficient immobilization of acetylcholinesterase (AChE) on silica sol–gel (SiSG) film assembling multiwall carbon nanotubes (MWNTs). The sol–gel matrix provided a biocompatible microenvironment around the enzyme and efficiently prevented leakage of the enzyme from the film. In the presence of acetylthiocholine chloride (ATCl) as a substrate, MWNTs promoted electron transfer reactions at a lower potential and catalyzed electrochemical oxidation of enzymatically formed thiocholine, thus increasing detection sensitivity. Based on the inhibition of organophosphorous compound on the enzymatic activity of AChE, using triazophos as a model compound, the effects of pH, temperature, and MWNTs contents were explored. Under optimum conditions, the inhibition of triazophos was proportional to its concentration from 0.02 μM to 1 μM and from 5 μM to 30 μM, with a detection limit of 0.005 μM. The determination of triazophos in garlic samples showed acceptable accuracy. Fabrication reproducibility of the sensor was good and stability was acceptable. The sensor is a promising new tool for pesticide analysis.


Acetylcholinesterase Amperometric determination Biosensor Multiwall carbon nanotube Sol–gel Triazophos 



The authors gratefully acknowledge the financial support of the Natural Science Foundation of Hubei Province (No. 2006ABA183) and the National Natural Science Foundation of China (No. 20672043).


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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  1. 1.Key Laboratory of Pesticide & Chemical Biology of Ministry of EducationCentral China Normal UniversityWuhanP.R. China
  2. 2.The Technology Center of Wuhan Iron & Steel CompanyWuhanP.R. China

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