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CircRNA HLCS regulates lens epithelial cell apoptosis via miR-338-3p/BPNT1 axis

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Abstract

Purpose

The purpose of this study was to investigate the effect of circ_HLCS on age-related cataract (ARC).

Methods

Circ_HLCS, microRNA (miR)-338-3p, and bisphosphate 3′-nucleotidase 1 (BPNT1) were quantified by quantitative real-time polymerase chain reaction or western blot. Cell proliferation and cell viability were assessed by the 5-Ethynyl-2′-deoxyuridinr and cell counting kit-8 assays. Cell apoptosis was detected by flow cytometry. Targeted correlations among circ_HLCS, miR-338-3p, and BPNT1 were verified by the dual-luciferase reporter and RNA pull-down assays.

Results

circ_HLCS was diminished in ARC tissues and UV-treated SRA01/04 cells. Elevated content of circ_HLCS undermined UV-induced cell proliferation inhibition and apoptosis. Mechanistically, circ_HLCS directly targeted miR-338-3p, and circ_HLCS regulated BPNT1 expression through miR-338-3p. Furthermore, reduction of miR-338-3p ameliorated UV-induced SRA01/04 cell damage by increasing BPNT1 expression.

Conclusion

Taken together, these data suggested that circ_HLCS inhibited apoptosis of UV-treated SRA01/04 cells by miR-338-3p/BPNT1 axis. Therefore, circ_HLCS might be a potential therapeutic target for ARC.

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Data availability

The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.

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Acknowledgements

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Funding

This work was supported by the Fund of Xi’an Science and Technology Bureau (NO. 20YXYJ0008).

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Authors and Affiliations

  1. Department of Ophthalmology, Xi’an People’s Hospital (Xi’an Fourth Hospital), Shaanxi Eye Hospital, Affiliated Guangren Hospital, School of Medicine, Xi’an Jiaotong University, No. 21 Jiefang Road, Xi’an, 710004, China

    Lianyi Sun, Fengzhi Li, Shuwei Bai & Chunchao Bi

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  1. Lianyi Sun
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Contributions

Conceptualization and Methodology: LS, FL and SB; Formal analysis and Data curation: SB and CB; Validation and Investigation: LS and FL; Writing—original draft preparation and Writing—review and editing: LS, FL, and SB; Approval of final manuscript: all authors.

Corresponding author

Correspondence to Shuwei Bai.

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The authors declare that they have no competing interests.

Ethical approval

The present study was approved by the Ethical Review Committee of Xi’an People’s Hospital (Xi’an Fourth Hospital).

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Written informed consent was obtained from all enrolled patients.

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Sun, L., Li, F., Bai, S. et al. CircRNA HLCS regulates lens epithelial cell apoptosis via miR-338-3p/BPNT1 axis. Int Ophthalmol 44, 142 (2024). https://doi.org/10.1007/s10792-024-03082-0

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