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Biochemical component analysis of human myopic corneal stroma using the Raman spectrum

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Abstract

Purpose

This study aimed to measure the Raman spectrum of the human corneal stroma lens obtained from small incision lenticule extraction surgery (SMILE) in Asian myopic eyes using a confocal Raman micro-spectrometer built in the laboratory.

Methods

Forty-three myopic patients who underwent SMILE with equivalent diopters between − 4.00 and − 6.00 D were selected, and the right eye data were collected. Corneal stroma lenses were obtained during surgery, and the Raman spectra were measured after air drying. The complete Raman spectrum of human myopic corneal stroma lens tissue was obtained within the range of 700–4000 cm−1.

Results

Thirteen characteristic peaks were found, with the stronger peaks appearing at 937 cm−1, corresponding to proline, valine, and the protein skeleton of the human myopic corneal stroma lens; 1243 cm−1, corresponding to collagen protein; 1448 cm−1, corresponding to the collagen protein and phospholipids; and 2940 cm−1, corresponding to the amino acid and lipids, which was the strongest Raman peak.

Conclusion

These results demonstrated that Raman spectroscopy has much potential as a fast, cost-effective, and reliable diagnostic tool in the diagnosis and treatment of eye diseases, including myopia, keratoconus, and corneal infection.

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Funding

This study has been supported by the Xi’an Science and Technology Plan Project of China (No. 22YXYJ0054), the Key Research and Development Program of Shaanxi (2021ZDLSF04-05), the National and Shaanxi Young Talent Program. The funding organization had no role in the design or conduct of this research.

Author information

Author notes

  1. Jing Li, Zhe Yu, Ming Han, Yong Li and Xueli Chen have contributed equally to this work.

Authors and Affiliations

  1. Shaanxi Eye Hospital, Xi’an People’s Hospital (Xi’an Fourth Hospital, Affliated People’s Hospital of Northwest University, No 4. Jiefang Road, Xin-Chen District, Xi’an, 710004, Shaanxi, China

    Jing Li, Zhe Yu, Yaohua Zhang, Shengsheng Wei, Liping Wu, Jing Du, Juan Li, Jinrong Gao & Yong Li

  2. Xi’an Key Laboratory of Intelligent Sensing and Regulation of Trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi’an, 710126, Shaanxi, China

    Ming Han, Qi Zeng & Xueli Chen

  3. Engineering Research Center of Molecular & Neuro Imaging of the Ministry of Education, Xidian University, Xi’an, 710126, Shaanxi, China

    Ming Han, Qi Zeng & Xueli Chen

Authors
  1. Jing Li
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  2. Zhe Yu
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  11. Yong Li
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Contributions

JL, YL, and XLC contributed to the concept and design; JL, ZY, and MH were involved in the analysis of the data; JL, ZY, and YHZ assisted in writing the article; SSW, LPW, and YL contributed to the critical revision of the article; QZ, JD, JL, JRG, and YL were involved in the data collection; JL, YL, and YHZ contributed to the provision of materials, patients, or resources; and MH, QZ, YL, and XLC assisted in the administrative, technical, or logistic support. All authors have reviewed the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Yong Li or Xueli Chen.

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Conflict of interest

The authors have no relevant financial or non-financial interests to disclose.

Ethical approval

The study protocol adhered to the tenets of the Declaration of Helsinki and was approved by the Xi’an People’s Hospital (Xi’an Fourth Hospital)’s Ethics Committee (approval number: 20220040).

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Written informed consent was obtained from at least one parent or legal guardian of each subject.

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Li, J., Yu, Z., Han, M. et al. Biochemical component analysis of human myopic corneal stroma using the Raman spectrum. Int Ophthalmol 44, 153 (2024). https://doi.org/10.1007/s10792-024-03034-8

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