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In Vitro efficacy of cyclosporine a and various antiseptics and antiviral drugs on adenovirus genotype 8, a common cause of epidemic keratoconjunctivitis

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Abstract

Purpose

To evaluate the in vitro efficacy of cidofovir, ganciclovir, povidone-iodine, chlorhexidine, and cyclosporine A on adenovirus genotype 8.

Methods

Conjunctival samples were collected from patients with adenoviral conjunctivitis and cultured in A549 cells. Adenovirus diagnosis was confirmed by RT-PCR. For each drug, the 50% cytotoxic concentration (CC 50 ) was determined. Subsequently, the antiviral activity was tested at concentrations below CC 50, and the 50% inhibitor concentration (IC 50 ) of drugs was determined

Results

While the IC 50 of cidofovir against adenovirus genotype 8 was 3.07 ± 0.8 µM, ganciclovir, povidone-iodine, chlorhexidine, and cyclosporine A were not found to be effective against adenovirus genotype 8 at concentrations below the CC 50 value.

Conclusions

Cidofovir was found effective and the IC 50 value was within the ranges in the literature. Ganciclovir and cyclosporine A were found to be ineffective at doses below the cytotoxic dose, povidone-iodine and chlorhexidine was found to be highly cytotoxic.

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Funding

This study was supported by Cukurova University, Scientific Research Projects Unit. Project No: TTU-2019–12009.

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Authors and Affiliations

  1. Department of Ophthalmology, Adana City Training and Research Hospital, Adana, Turkey

    Aynura Sariyeva Aydamirov

  2. Department of Ophthalmology, School of Medicine, Cukurova University, Adana, Turkey

    Ibrahim Inan Harbiyeli, Elif Erdem & Meltem Yagmur

  3. Division of Virology, Department of Microbiology, School of Medicine, Cukurova University, Adana, Turkey

    Gokhan Ozturk & Fugen Yarkin

Authors
  1. Aynura Sariyeva Aydamirov
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  2. Ibrahim Inan Harbiyeli
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  3. Gokhan Ozturk
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  4. Fugen Yarkin
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  5. Elif Erdem
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  6. Meltem Yagmur
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Gokhan Ozturk and Fugen Yarkin. The first draft of the manuscript was written by Aynura Sariyeva Aydamirov and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Aynura Sariyeva Aydamirov.

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The study was compliant with the Declaration of Helsinki and additional approval was obtained from the Ethics Committee of Cukurova University, Medical Faculty, Adana, Turkey. (Number of meetings: 87; number of decisions: 9).

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Aydamirov, A.S., Harbiyeli, I.I., Ozturk, G. et al. In Vitro efficacy of cyclosporine a and various antiseptics and antiviral drugs on adenovirus genotype 8, a common cause of epidemic keratoconjunctivitis. Int Ophthalmol 43, 1701–1710 (2023). https://doi.org/10.1007/s10792-022-02567-0

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