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β-asarone attenuates the proliferation, migration and enhances apoptosis of retinoblastoma through Wnt/β-catenin signaling pathway

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Abstract

Purpose

β-asarone is the prime component of essential oil extracted from Acori graminei Rhizoma, which plays an inhibitory role in various tumors. Here, we aim to investigate the functions as well as the mechanism of β-asarone in retinoblastoma (RB).

Methods

RB cell lines SO-Rb50 and HXO-Rb44 were treated with different doses of β-asarone. Then, CCK8 and BrdU experiments were adopted to examine the RB cell proliferation. Wound healing test and Transwell assay were employed to detect cell migration and invasion. RB cell apoptosis was tested by flow cytometry and Western blot. An RB cell xenograft model was constructed on nude mice for testing the role of β-asarone on RB cell growth in vivo. RT-PCR and Western blot were used to determine the effect of β-asarone on Wnt/β-catenin signaling pathway. Furthermore, the Wnt/β-catenin pathway inhibitor PNU-74654 and activator HLY78 were administered to RB cells for confirming the effects of β-asarone in Wnt/β-catenin pathway.

Results

In vivo experiment showed that β-asarone attenuated SO-Rb50 cell growth in nude mice. Further research found that β-asarone significantly repressed Wnt/β-catenin canonical pathway activation.

Conclusion

Prior inhibition of Wnt/β-catenin pathway abolished the antitumor effects induced by β-asarone. β-asarone exerted antitumor effects in RB cells by inactivating the Wnt/β-catenin signaling pathway.

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Availability of data and materials

The data sets used and analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

RB:

Retinoblastoma

ERK:

Extracellular signal-regulated kinase

AD:

Alzheimer's disease

MAT:

Matrine

KIF18B:

Kinesin family member 18B

DMSO:

Dimethylsulfoxide

CCK8:

Cell Counting Kit 8

OD:

Optical density

BrdU:

Bromo-2′-deoxyuridine

DAPI:

Dianmidino-2-phenylindole

LiCl:

Lithium chloride

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Acknowledgements

Not applicable.

Funding

This study was supported by Fund of Xi’an Science and Technology Bureau (20YXYJ0008(2)).

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Authors and Affiliations

  1. Ophthalmology Department, Shaanxi Eye Hospital, Xi’an People’s Hospital (Xi’an Fourth Hospital), Affiliated Guangren Hospital, School of Medicine, Xi’an Jiaotong University, No.21 Jiefang Road, Xi’an, 710004, Shaanxi, China

    Shuwei Bai, Juan Shao, Chunchao Bi & Fengzhi Li

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  1. Shuwei Bai
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  2. Juan Shao
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  3. Chunchao Bi
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  4. Fengzhi Li
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Contributions

SB and JS conceived and designed the experiments; SB, FL and FL performed the experiments; Statistical analysis was contributed by FL and CB; CB wrote the paper. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Chunchao Bi.

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The authors have no relevant financial or non-financial interests to disclose.

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Our study was approved by the Ethics Review Board of Shaanxi Eye Hospital.

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Bai, S., Shao, J., Bi, C. et al. β-asarone attenuates the proliferation, migration and enhances apoptosis of retinoblastoma through Wnt/β-catenin signaling pathway. Int Ophthalmol 43, 1687–1699 (2023). https://doi.org/10.1007/s10792-022-02566-1

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  • DOI: https://doi.org/10.1007/s10792-022-02566-1

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