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Role of oncogenic viruses in the development ocular surface squamous neoplasia

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Abstract

Purpose

The pathogenesis of ocular surface squamous neoplasia is not fully understood. Therefore, we evaluated the role of oncogenic viruses in the pathogenesis of ocular surface squamous neoplasia in Israel.

Methods

Patients with ocular surface squamous neoplasia were enrolled in this retrospective study. The specimens were taken during 2004–2015 from two big centers: Emek Medical Center, Afula and the Hadassah Medical Center, Jerusalem. All the specimens (totally 26) were analyzed by immunohistochemistry for evidence of oncogenic viruses that included Human Papilloma virus, Herpes Simplex virus and Cytomegalovirus infection and 14 samples were examined by polymerase chain reaction. In addition, all the samples were examined for Epstein Barr virus infection by immunohistochemistry and Epstein Barr encoding region test.

Results

Twenty-six patients were included with a mean age of 61.81 \(\pm\) 3.83 years (mean \(\pm {\text{SE}})\). Immunohistochemistry staining and Epstein Barr encoding region test did not detect any of the oncogenic viruses in the 26 samples. Human Papilloma virus-16 and -18, and Herpes Simplex virus were detected by polymerase chain reaction in 14.2%, 7% and 7%, respectively.

Conclusion

We conclude from our study that oncogenic viruses may play a role in the pathogenesis of ocular surface squamous neoplasia in Israel.

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Data availability

The authors declare that all data and materials support their published claims and comply with field standards.

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Acknowledgements

We thank Dr. Yuval Eitan, Yifat Guthmann and Hila Belhanes-Peled from the laboratory of the Institute of Tissue Diagnostics and Cancer Research for technical assistance.

Funding

The authors did not receive support from any organization for the submitted work.

Author information

Author notes

  1. Daniel Briscoe and Judit Krausz have contributed equally.

Authors and Affiliations

  1. Department of Ophthalmology, Baruch Padeh Medical Center, Dov Hoz 0, Tiberias, Poriya, Israel

    Shirin Hamed-Azzam

  2. The Institute of Tissue Diagnostics and Cancer Research, Emek Medical Center, Afula, Israel

    Natalia Edison, Irit Elmalah & Judit Krausz

  3. Department of Ophthalmology, Emek Medical Center, Afula, Israel

    Daniel Briscoe, Abed Mukari & Hector Camacho

  4. Division of Ophthalmology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel

    Shahar Frenkel

  5. The Wohl Institute for Translational Medicine, Hadassah-Hebrew University Medical Center, Jerusalem, Israel

    Shahar Frenkel

  6. Microbiology Laboratory, Emek Medical Center, Afula, Israel

    Meirav Strauss

Authors
  1. Shirin Hamed-Azzam
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  2. Natalia Edison
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  3. Daniel Briscoe
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  4. Shahar Frenkel
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  6. Meirav Strauss
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  7. Hector Camacho
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  8. Irit Elmalah
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  9. Judit Krausz
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Dr. Shirin Hamed-Azzam, Dr. Natalia Edison and Dr. Judit Krausz. The first draft of the manuscript was written by Dr. Shirin Hamed Azzam and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Shirin Hamed-Azzam.

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Conflict of interest

The authors declare no conflict of interest.

Ethical approval

The research was approved by the ethics committee of the two medical centers in compliance with the declaration of Helsinki. The procedures used in this study adhere to the tenets of the declaration of Helsinki.

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Consent was not obtained as the study included pathology slides.

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Hamed-Azzam, S., Edison, N., Briscoe, D. et al. Role of oncogenic viruses in the development ocular surface squamous neoplasia. Int Ophthalmol 41, 3599–3605 (2021). https://doi.org/10.1007/s10792-021-01933-8

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  • DOI: https://doi.org/10.1007/s10792-021-01933-8

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