Structural features of eyelid connective tissue in patients with primary open-angle glaucoma

Abstract

Purpose

To study the connective tissue (CT) structure of upper eyelid skin of primary open-angle glaucoma (POAG) patients.

Patients and methods

Forty-seven patients aged 47–91 expecting blepharoplasty formed 3 groups: group 1 [16 subjects without POAG, median age 55 years (interquartile range 54–55.5)], group 2 [12 subjects without POAG, median age 73 (72–76.5)], and group 3 [(19 subjects with POAG, median age 74 (70–80.5)]. Age differences between groups 1 and 2 and groups 1 and 3 are significant (p < 0.05). Thermodynamic parameters of skin samples taken during blepharoplasty: Endothermic peak (\(T_{d}\), °C) and denaturation enthalpy (\(\Delta H_{d}\), J/g of dry weight) were determined using differential scanning calorimetry.

Results

\(\Delta H_{d}\) and \(T_{d}\) in groups 1–3 were, respectively, 8.41 (7.42–10.25) and 66.55 (59.9–66.7); 7.10 (5.76–10.17) and 67.35 (67.0–68.03); 11.40 (9.0–14.9) and 67.70 (67.05–68.45). \(T_{d}\) differences between groups 1 and 2 are significant (p < 0.05), and Spearman’s correlation between the age and \(T_{d}\) is direct, medium (R = 0.638) and significant. \(\Delta H_{d}\) in group 3 is significantly higher than in group 2. \(\Delta H_{d}\) and \(T_{d}\) in patients without POAG (groups 1 and 2) and those with POAG (group 3) are, respectively, 7.79 (6.9–10.17) and 66.6 (61.2–67,3); 11.40 (9.0–14.9); 67.7 (67.05–68.45); the respective differences are significant.

Conclusion

Patients without POAG show a significant increase in \(T_{d}\) with age, while \(\Delta H_{d}\) slightly decreases. In POAG, \(\Delta H_{d}\) is significantly higher and \(T_{d}\) tends to grow, which may indicate structural changes in eyelid CT (collagen accumulation and cross-linking level rise). Since the upper lid is unaffected by increasing IOP directly, the changes may be viewed as manifestations of systemic CT pathology.

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Correspondence to L. A. Svetikova.

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Svetikova, L.A., Iomdina, E.N., Ignatyeva, N.Y. et al. Structural features of eyelid connective tissue in patients with primary open-angle glaucoma. Int Ophthalmol 39, 2005–2014 (2019). https://doi.org/10.1007/s10792-018-1035-7

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Keywords

  • Glaucoma
  • Collagen
  • Cross-links
  • Connective tissue
  • Eyelid skin