Abstract
Purpose
In the present study, we aimed to evaluate the expression of EP receptors in primary and recurrent human pterygium tissues.
Methods
Pterygium samples were collected from 65 patients with primary pterygium and 16 patients with recurrent pterygium. Normal conjunctival tissues were collected from nasal interpalpebral area from 17 patients without systemic and any other ocular pathology. Expression of EP receptors was evaluated by immunohistochemistry. The median value for each receptor staining score (RSS) was determined in normal conjunctival specimens. In this study, RSS of > median value was defined as positive staining or high expression and ≤ median value as negative staining or weak expression in specimens. Chi-square test was used for statistical analysis, and p value of less than 0.05 was considered significant.
Results
Stromal expression of EP1 was significantly higher in primary and recurrent pterygium specimens compared to normal conjunctival tissues (p = 0.007 and p = 0.002, respectively). Epithelial expressions of EP2 and EP3 were significantly lower in primary pterygium specimens compared to normal conjunctival tissues (p = 0.005 and p < 0.0001, respectively), and stromal expressions were insignificant. Stromal expression of EP4 was significantly higher in primary and recurrent pterygium specimens compared to normal conjunctival tissues (p = 0.002 and p = 0.012, respectively).
Conclusions
Expression of EP receptors has been up- or downregulated in primary and recurrent pterygium tissues, and these receptors may play a role in formation and recurrence of pterygium.
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References
Bradley JC, Yang W, Bradley RH et al (2010) The science of pterygia. Br J Ophthalmol 94:815–820
Weinstein O, Rosenthal G, Zirkin H et al (2002) Overexpression of p53 tumor suppressor gene in pterygia. Eye 16:619–621
Liu T, Liu Y, Xie L et al (2013) Progress in the pathogenesis of pterygium. Curr Eye Res 38:1191–1197
Di Girolamo N, Kumar RK, Coroneo MT et al (2002) UVB-mediated induction of interleukin-6 and -8 in pterygium and cultured human pterygium epithelial cells. Invest Ophthalmol Vis Sci 43:3430–3437
Kria L, Ohira A, Amemiya T (1996) Immunohistochemical localization of basic fibroblast growth factor, platelet derived growth factor, transforming growth factor- beta and tumor necrosis factor-alpha in pterygium. Acta Histochem 98:195–201
Kase S, Osaki M, Sato I et al (2007) Immunolocalisation of E-cadherin and beta- catenin in human pterygium. Br J Ophthalmol 91:1209–1212
Ricciotti E, Fitzgerald GA (2011) Prostaglandins and inflammation. Arterioscler Thromb Vasc Biol 31:986–1000
Narumiya S, Fitz Gerald GA (2001) Genetic and pharmacological analysis of prostanoid receptor function. J Clin Investig 108:25–30
Schlötzer SU, Zenkel M, Nüsing RM (2002) Expression and localization of FP and EP prostanoid receptor subtypes in human ocular tissues. Investig Ophthalmol Vis Sci 43:1475–1487
Biswas S, Bhattacherjee P, Paterson CA (2004) Prostaglandin E2 receptor subtypes, EP1, EP2, EP3 and EP4 in human and mouse ocular tissues-a comparative immunohistochemical study. Prostaglandins Leukot Essent Fat Acids 71:277–288
Shiraishi H, Yoshida H, Saeki K et al (2008) Prostaglandin E2 is a major soluble factor produced by stromal cells for preventing inflammatory cytokine production from dentritic cells. Int Immunol 20:1219e29
Venza I, Visalli M, Cucinotta M et al (2013) NOD2 triggers PGE2 synthesis leading to IL-8 activation in staphylococcus aureus-infected human conjunctival epithelial cells. Biochem Biophys Res Commun 440:551–557
Ueta M, Matsuoka T, Yokoi N et al (2011) Prostaglandin E2 suppresses polyinosine- polycytidylic acid (polyI:c)-stimulated cytokine production via prostaglandin E2. Br J Ophthalmol 95:859–863
Bianchi E, Scarinci F, Grande C et al (2012) Immunohistochemical profile of VEGF, TGF-ß and PGE2 in human pterygium and normal conjunctiva: experimental study and review of the literature. Int J Immunopathol Pharmacol 25:607–615
Putti TC, Teh M, Lee YS (2004) Biological behavior of keratoacanthoma and squamous cell carcinoma: telomerase activity and COX-2 as potential markers. Mod Pathol 17:468–475
Kim KW, Park SH, Kim JC (2016) Fibroblast biology in pterygia. Exp Eye Res 142:32–39
Chui J, Coroneo MT, Tat LT et al (2011) Ophthalmic pterygium. Am J Pathol 178:817–827
Utea M, Matsuoka T, Narumiya S et al (2009) Prostaglandin receptor subtype EP3 in conjunctival epithelium regulates late-phase reaction of experimental allergic conjunctivitis. J Allergy Clin Immunol 123:466–471
Ueta M (2010) Regulation of ocular surface inflammation by prostaglandin E receptor subtype EP3. Cornea 29(Suppl 1):57–61
Ueta M, Sotozono C, Yokoi N et al (2010) Prostaglandin E receptor 4 expression in human conjunctival epithelium and its downregulation in devastating ocular surface inflammatory disorders. Arch Ophthalmol 128:1369–1371
Yokoyama U, Iwatsubo K, Umemura M et al (2013) The prostanoid EP4 receptor and its signaling pathway. Pharmacol Rev 65:1010–1052
Adiguzel U, Karabacak T, Sari A et al (2007) Cyclooxygenase-2 expression in primary and recurrent pterygium. Eur J Ophthalmol 17:879–884
Chiang CC, Cheng YW, Lin CL et al (2007) Cyclooxygenase-2 expression in pterygium. Mol Vis 13:635–638
Surh I, Rundhaug JE, Pavone A et al (2012) The EP1 receptor for prostaglandin E2 promotes the development and progression of malignant murine skin tumors. Mol Carcinog 51:553–564
Tober KL, Wilgus TA, Kusewitt DF et al (2006) Importance of the EP(1) receptor in cutaneous UVB-induced inflammation and tumor development. J Investig Dermatol 126:205–211
Brouxhon S, Konger RL, VanBuskirk J et al (2007) Deletion of prostaglandin E2 EP2 receptor protects against ultraviolet-induced carcinogenesis, but increases tumor aggressiveness. J Investig Dermatol 127:439–446
Simper MS, Rundhaug JE, Mikulec C et al (2014) The tumor promoting activity of the EP4 receptor for prostaglandin E2 in murine skin. Mol Oncol 8:1626–1639
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Dinç, E., Dursun, Ö., Yılmaz, B. et al. Expression of prostaglandin E2 receptor subtypes in human pterygium and normal conjunctiva: immunohistochemical study. Int Ophthalmol 38, 1703–1708 (2018). https://doi.org/10.1007/s10792-017-0651-y
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DOI: https://doi.org/10.1007/s10792-017-0651-y