International Ophthalmology

, Volume 38, Issue 1, pp 75–82 | Cite as

Evaluation of the effect of fluorescein angiography on retinal vessel diameter: an optical coherence tomography study

  • Metin Unlu
  • Duygu Gülmez Sevim
  • Cagatay Karaca
  • Bahadir Duzgun
  • Ayse Ozturk Oner
  • Ertugrul Mirza
Original Paper



To evaluate the effect of fluorescein angiography on retinal vessel diameter with Optical Coherence Tomography (OCT).


In this cross-sectional study, a total of 81 eyes of 81 patients who were performed fluorescein angiography (FA) procedure were included. Retinal vessels were examined with the Spectral-domain OCT at baseline and immediately after FA procedure. A cube scan consisting of seven horizontal scans were placed at the inferior border of the disk to include the inferior temporal retinal vessels. Vessels diameters were measured at five measurement points (480–1440 μm inferiorly from the optic disk border).


The mean age of the study subjects was 58.02 ± 14.1 years. At baseline, the mean diameter of the retinal artery was 120.16 ± 24.56 µm and of the vein 157.94 ± 32.34 µm at the measurement point of 480 μm, with a gradual decrease to 114.91 ± 25.59 and 152.17 ± 28.17 µm, respectively, at 1440 μm. After FA procedure, the mean diameter of the retinal artery was 122.85 ± 26.35 and of the vein 158.30 ± 32.21 µm at the measurement point of 480 μm, with a gradual decrease to 115.22 ± 22.91 and 151.94 ± 28.93 µm, respectively, at 1440 μm. There were no statistical differences for either of these comparisons at any of the points of both artery and vein measurements.


There was not any clinically significant change in retinal artery diameter such as a dilatatory response after FA procedure in patients with hypertension, diabetes, and age-related macular degeneration (AMD).


Fluorescein angiography Optical coherence tomography Retinal vessel diameter 


Compliance with ethical standards

Conflict of interest

None of the authors has conflict of interest with this submission.

Financial disclosure

None of the authors has financial interest in any material or method mentioned.


  1. 1.
    Antonetti DA, Klein R, Gardner TW (2012) Diabetic retinopathy. N Engl J Med 366:1227–1239CrossRefPubMedGoogle Scholar
  2. 2.
    Wong TY, Mitchell P (2004) Hypertensive retinopathy. N Engl J Med 351:2310–2317CrossRefPubMedGoogle Scholar
  3. 3.
    Nagel E, Vilser W (2004) Autoregulative behavior of retinal arteries and veins during changes of perfusion pressure: a clinical study. Graefes Arch Clin Exp Ophthalmol 242:13–17CrossRefPubMedGoogle Scholar
  4. 4.
    Nagel E, Vilser W, Lanzl I (2004) Age, blood pressure, and vessel diameter as factors influencing the arterial retinal flicker response. Invest Ophthalmol Vis Sci 45:1486–1492CrossRefPubMedGoogle Scholar
  5. 5.
    Wong TY, Knudtson MD, Klein R, Klein BE, Meuer SM, Hubbard LD (2004) Computer-assisted measurement of retinal vessel diameters in the Beaver Dam Eye Study: methodology, correlation between eyes, and effect of refractive errors. Ophthalmology 111:1183–1190CrossRefPubMedGoogle Scholar
  6. 6.
    Triantafyllou A, Doumas M, Anyfanti P, Gkaliagkousi E, Zabulis X, Petidis K, Gavriilaki E, Karamaounas P, Gkolias V, Pyrpasopoulou A et al (2013) Divergent retinal vascular abnormalities in normotensive persons and patients with never-treated, masked, white coat hypertension. Am J Hypertens 26:318–325CrossRefPubMedGoogle Scholar
  7. 7.
    Soliman W, Vinten M, Sander B, Soliman KA, Yehya S, Rahman MS, Larsen M (2008) Optical coherence tomography and vessel diameter changes after intravitreal bevacizumab in diabetic macular oedema. Acta Ophthalmol 86:365–371CrossRefPubMedGoogle Scholar
  8. 8.
    Kotliar KE, Mucke B, Vilser W, Schilling R, Lanzl IM (2008) Effect of aging on retinal artery blood column diameter measured along the vessel axis. Invest Ophthalmol Vis Sci 49:2094–2102CrossRefPubMedGoogle Scholar
  9. 9.
    Fischer MD, Huber G, Feng Y, Tanimoto N, Muhlfriedel R, Beck SC, Troger E, Kernstock C, Preising MN, Lorenz B et al (2010) In vivo assessment of retinal vascular wall dimensions. Invest Ophthalmol Vis Sci 51:5254–5259CrossRefPubMedGoogle Scholar
  10. 10.
    Guimaraes P, Rodrigues P, Celorico D, Serranho P, Bernardes R (2015) Three-dimensional segmentation and reconstruction of the retinal vasculature from spectral-domain optical coherence tomography. J Biomed Opt 20:016006CrossRefPubMedGoogle Scholar
  11. 11.
    Golzan SM, Avolio A, Graham SL (2011) Minimising retinal vessel artefacts in optical coherence tomography images. Comput Methods Programs Biomed 104:206–211CrossRefPubMedGoogle Scholar
  12. 12.
    Pilch M, Wenner Y, Strohmayr E, Preising M, Friedburg C, Zu Bexten EM, Lorenz B, Stieger K (2012) Automated segmentation of retinal blood vessels in spectral domain optical coherence tomography scans. Biomed Opt Express 3:1478–1491CrossRefPubMedCentralPubMedGoogle Scholar
  13. 13.
    Yannuzzi LA, Rohrer KT, Tindel LJ, Sobel RS, Costanza MA, Shields W, Zang E (1986) Fluorescein angiography complication survey. Ophthalmology 93:611–617CrossRefPubMedGoogle Scholar
  14. 14.
    Early Treatment Diabetic Retinopathy Study Research Group (1991) Grading diabetic retinopathy from stereoscopic color fundus photographs—an extension of the modified Airlie House classification: ETDRS report number 10. Ophthalmology 98:786–806CrossRefGoogle Scholar
  15. 15.
    Terai N, Haustein M, Siegel A, Stodtmeister R, Pillunat LE, Sandner D (2014) Diameter of retinal vessels in patients with diabetic macular edema is not altered by intravitreal ranibizumab (lucentis). Retina 34:1466–1472CrossRefPubMedGoogle Scholar
  16. 16.
    Goldenberg D, Shahar J, Loewenstein A, Goldstein M (2013) Diameters of retinal blood vessels in a healthy cohort as measured by spectral domain optical coherence tomography. Retina 33(9):1888–1894CrossRefPubMedGoogle Scholar
  17. 17.
    Ozcimen M, Sakarya Y, Goktas S, Sakarya R, Alpfidan I, Yener HI, Demir LS (2015) The effect of rebreathing and hyperventilation on retinal and choroidal vessels measured by spectral domainoptical coherence tomography. Cutan Ocul Toxicol 34(4):313–317CrossRefPubMedGoogle Scholar
  18. 18.
    Maurice DM (1967) The use of fluorescein in ophthalmological research. Invest Ophthalmol 6:464–477PubMedGoogle Scholar
  19. 19.
    Kwiterovich KA, Maguire MG, Murphy RP, Schachat AP, Bressler NM, Bressler SB, Fine SL (1991) Frequency of adverse systemic reactions after fluorescein angiography. Results of a prospective study. Ophthalmology 98:1139–1142CrossRefPubMedGoogle Scholar
  20. 20.
    Knudsen LL, Nielsen-Kudsk F (1998) Anterior chamber and vitreous fluorescein kinetics in normal and diabetic subjects. Acta Ophthalmol Scand 76:396–400CrossRefPubMedGoogle Scholar
  21. 21.
    Shao Q, Heussen FM, Ouyang Y, Hager A (2016) Retinal vessel diameter changes in different severities of diabetic retinopathy by SD-OCT. Eur J Ophthalmol 26(4):342–346CrossRefPubMedGoogle Scholar
  22. 22.
    Mendrinos E, Mangioris G, Papadopoulou DN, Donati G, Pournaras CJ (2013) Long-term results of the effect of intravitreal ranibizumab on the retinal arteriolar diameter in patients with neovascular age-related macular degeneration. Acta Ophthalmol 91:e184–e190CrossRefPubMedGoogle Scholar
  23. 23.
    Schuster AK, Fischer JE, Vossmerbaeumer C, Vossmerbaeumer U (2015) Optical coherence tomography-based retinal vessel analysis for the evaluation of hypertensive vasculopathy. Acta Ophthalmol 93:e148–e153CrossRefPubMedGoogle Scholar
  24. 24.
    Muraoka Y, Tsujikawa A, Kumagai K, Akiba M, Ogino K, Murakami T, Akagi-Kurashige Y, Miyamoto K, Yoshimura N (2013) Age- and hypertension-dependent changes in retinal vessel diameter and wall thickness: an optical coherence tomography study. Am J Ophthalmol 156:706–714CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Metin Unlu
    • 1
  • Duygu Gülmez Sevim
    • 1
  • Cagatay Karaca
    • 1
  • Bahadir Duzgun
    • 1
  • Ayse Ozturk Oner
    • 1
  • Ertugrul Mirza
    • 1
  1. 1.Ophthalmology Department, School of MedicineErciyes UniversityKayseriTurkey

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