Abstract
The influence of human atrial natriuretic peptide (ANP) and of two related peptides, human brain natriuretic peptide (BNP) and urodilatin (URO) on the bronchoconstriction induced by inhalation of histamine in conscious, non-anaesthetized guinea pigs was tested.
Changes in lung function were registered using two independent methods, one operating in a closed body-plethysmographic system, the other in an open system based on the time lag of air flow curves. The peptides were infused (0.25 ml/min) into the jugular vein for a period from 10 min before until 15 min after the histamine inhalation.
ANP displayed virtually no effect on the bronchoconstriction. URO showed some inibition at 1280ng kg−1 min−1, but not at lower doses. BNP (640ng kg−1 min−1) inhibited the bronchoconstriction markedly for the total registration period.
It can be concluded from these results that BNP exerts bronchoprotective effects in the conscious guinea pig, which are superior to those of ANP or URO.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
De Bold AJ, Borenstein HB, Veress AT, Sonnenberg H. A rapid and potent natriuretic response to intravenous injection of atrial myocardial extracts in rats. Life Sci. 1981;28:89–94.
Currie MG, Geller DM, Cole BR et al. Bioactive cardiac substances: potent vasorelaxant activity in mammalian atria. Science. 1983;221:71–3.
Kangawa K, Matsuo H. Purification and complete amino acid sequence of α-human atrial natriuretic polypeptide (α-hANP). Biochem Biophys Res Commun. 1984;118(1):131–9.
Flynn TG, de Bold ML, de Bold AJ. The amino acid sequence of an atrial peptide with potent diuretic and natriuretic properties. Biochem Biophys Res Commun. 1983;117(3):859–65.
Seidah NG, Lazure C, Chretien M et al. Amino acid sequence of homologous rat atrial peptides: natriuretic activity of native and synthetic forms. Proc Natl Acad Sci USA. 1984;81:2640–4.
Harris PJ, Thomas D, Morgan TO. Atrial natriuretic peptide inhibits angiotensin-stimulated proximal tubular sodium and water reabsorption. Nature. 1987;326:697–8.
Rocha AS, Kudo LH. Atrial peptide and cGMP effects on NaCl transport in innermedullary collecting duct. Am J Physiol. 1990;259:F258-F68.
Cogan MG. Atrial natriuretic peptide can increase renal solute excretion primarily by raising glomerular filtration. Am J Physiol. 1986;250:F710-F14.
Winquist RJ, Faison EP, Nutt RF. Vasodilator profile of synthetic atrial natriuretic factor. Eur J Pharmacol. 1984;102:169–73.
Sasaki A, Kida O, Kangawa K, Matsuo H, Tanaka K. Involvement of sympathetic nerves in cardiosuppressive effects of human atrial natriuretic peptide in anesthetized rats. Eur J Pharmacol. 1986;120:345–9.
Oelkers W, Kleiner S, Bähr W. Effects of incremental infusions of atrial natriuretic factor on aldosterone, renin and blood pressure in humans. Hypertension. 1988;12:462–7.
Matsuoka H, Ishii M, Hirata Y et al. Evidence for lack of a role of cGMP in effect of α-hANP on aldosterone inhibition. Am J Physiol. 1987;252 (Endocrinol Metab. 15):E643-E7.
Samson WK. Atrial natriuretic factor inhibits dehydration- and hemorrhage-induced vasopressin release. Neuroendocrinology. 1985;40:277–9.
Sudoh T, Kangawa K, Minamino N, Matsuo H. A new natriuretic peptide in porcine brain. Nature. 1988;332(3):78–81.
Sudoh T, Minamino N, Kangawa K, Matsuo H. Brain natriuretic peptide-32: N-terminal six amino acid extended form of brain natriuretic peptide identified in porcine brain. Biochem Biophys Res Commun. 1988;155(2):726–32.
Aburaya M, Hino J, Minamino N, Kangawa K, Matsuo H. Isolation and identification of rat brain natriuretic peptides in cardiac atrium. Biochem Biophys Res Commun. 1989;163(1):226–32.
Dagnino L, Drouin J, Nemer M. Differential expression of natriuretic peptide genes in cardiac and extracardiac tissues. Mol Endocrinol. 1991;5:1292–300.
Hino J, Tateyama H, Minamino N, Kangawa K, Matsuo H. Isolation and identification of human brain natriuretic peptides in cardiac atrium. Biochem Biophys Res Commun. 1990;167(2):693–700.
Kambayashi Y, Nakao K, Mukoyama M et al. Isolation and sequence determination of human brain natriuretic peptide in human atrium. FEBS Lett. 1990;259:341–5.
Minamino N, Aburaya M, Ueda S, Kangawa K, Matsuo H. The presence of brain natriuretic peptide of 12,000 Daltons in porcine heart. Biochem Biophys Res Commun. 1988;155(2);740–6.
Minamino N, Kangawa, K, Matsuo H. Isolation and identification of a high molecular weight brain natriuretic peptide in porcine cardiac atrium. Biochem Biophys Res Commun. 1988;157(1):402–9.
Rosenzweig A, Seidman CE. Atrial natriuretic factor and related peptide hormones. Annu Rev Biochem. 1991;60:229–55.
Saito Y, Nakao K, Itoh H et al. Brain natriuretic peptide is a novel cardiac hormone. Biochem Biophys Res Commun. 1989;158(2):360–8.
Togashi K, Hirata Y, Ando K, Takei Y, Kawakami M, Marumo F. Brain natriuretic peptide-like immunoreactivity is present in human plasma. FEBS Lett. 1989;250(2):235–7.
Jennings DB, Flynn TG. The disulfide bonded ring of iso-rANP, unlike that of rANP, has potent cardiovascular activity. Can J Physiol Pharmacol. 1990;68:131–6.
Jennings DB, McKirdy JC, Ohtake PJ, Sarda IR, Flynn TG, Hira G. Infusion of iso-rANP (1-45) or (17–45) increases plasma immunoreactive ANP and lowers plasma renin activity and aldosterone. Can J Physiol Pharmacol. 1990;68:1292–7.
McGregor A, Richards M, Espiner E, Yandle T, Ikram H. Brain natriuretic peptide administered to man: actions and metabolism. J Clin Endocrinol Metab. 1990;70:1103–7.
Holmes SJ, Espiner EA, Richards AM, Yandle TG, Frampton C. Renal, endocrine, and hemodynamic effects of human brain natriuretic peptide in normal man. J Clin Endocrinol Metab. 1993;76:91–6.
Kambayashi Y, Nakao K, Kimura H et al. Biological characterisation of human brain natriuretic peptide (BNP) and rat BNP: species-specific actions of BNP. Biochem Biophys Res Commun. 1990;173(2):599–605.
Schulz-Knappe P, Forssmann K, Herbst F, Hock D, Pipkorn R, Forssmann WG. Isolation and structural analysis of ‘urodilatin’, a new peptide of the cardiodilatin-(ANP)-family, extracted from human urine. Klin Wochenschr. 1990;66:752–9.
Saxenhofer H, Raselli A, Weidmann P et al. Urodilatin, a natriuretic factor from kidneys, can modify renal and cardiovascular function in men. Am J Physiol. 1990;259:F832–8.
Kentsch M, Ludwig D, Drummer C, Gerzer R, Müller-Esch G. Haemodynamic and renal effects of urodilatin bolus injections in patients with congestive heart failure. Eur J Clin Invest. 1992;22:662–9.
O’Donnell M, Garippa R, Welton AF. Relaxant activity of atriopeptins in isolated guinea pig airway and vascular smooth muscle. Peptides. 1985;6(4):597–601.
Chou J, Kubota E, Sata T, Said SI. Comparative relaxant activities of atrial natriuretic peptides (ANPs) and vasoactive intestinal peptide (VIP) on smooth muscle structures in lung. Federation Proc. 1986;45(1):553.
Watanabe H, Suzuki K, Takagi K, Satake T. Mechanics of atrial natriuretic polypeptide and sodium nitroprusside-induced relaxation in guinea pig tracheal smooth muscle. Arzneim-Forsch/Drug-Res. 1990;40(II,nr.7):771–6.
Potvin W, Varma DR. Bronchodilator activity of atrial natriuretic peptide in guinea pigs. Can J Physiol Pharmacol. 1988;67:1213–18.
Ishii K, Murad F. ANP relaxes bovine tracheal smooth muscle and increases cGMP. Am J Physiol. 1989;256 (Cell Physiol. 25):C495–500.
Hulks G, Crabb KG, McGrath JC, Thomson NC. In vitro effects of atrial natriuretic factor and sodium nitroprusside on bronchomotor tone in human bronchial smooth muscle (Abstract). Am Rev Resp Dis. 1991;143:A344.
Labat C, Norel X, Beneviste J, Brink C. Vasorelaxant effects of atrial peptide II on isolated human pulmonary muscle preparations. Eur J Pharmacol. 1988;150:397–400.
Banerjee MR, Newman JH. Acute effects of atrial natriuretic peptide on lung mechanics and hemodynamics in awake sheep. J Appl Physiol. 1990;69(2):728–33.
Englebach IM, Lappe RW, Hand JM. Bronchoprotective and bronchodilator activity of anaritide (human atrial natriuretic factor [102–126]) infusion in the anesthetized guinea pig. Pulmon Pharmacol. 1988;1:119–23.
Hand JM, Englebach IM, Lappe RW. Anaritide [human atrial natriuretic factor (102–126)] as a bronchodilator in the anesthetized guinea pig. Am Rev Respir Dis. 1988;137:A28.
Amyot R, Lesiège D, Michoud MC, Larochelle P, Hamet P. Effect of atrial natriuretic factor on airway sensitivity to histamine in anesthetized dogs. Eur Respir J. 1989;2(Suppl. 5):301s.
Flüge T, Hoymann H, Hohlfeld J, Heinrich U, Fabel H, Wagner TOF. Protective effect of urodilatin (URO) or ANP against an acetylcholine (ACH)-induced bronchoconstriction in the rat in vivo. Naunyn-Schmiedeberg’s Arch Pharmacol. 1992;345 Suppl. 2:R51.
Robichaud A, Michoud MC, Saunier C, Duvivier C, Peslin R, du Souich P. Effect of atrial natriuretic peptide on bronchial tone in anesthetized rabbits. Peptides. 1993;14:1325–30.
Robichaud A, Saunier C, Michoud MC, du Souich P. Muscarinic effect of atrial natriuretic peptide on rabbit airways. Br J Pharmacol. 1993;110:804–8.
Haxhiu MA, Deal EC, van Lunteren E, Cherniack NS. Central effects of somatostatin and atrial natriuretic peptide on tracheal tone. J Appl Physiol. 1993;75:2353–9.
Hulks G, Jardine AG, Connell JMC, Thomson NC. Bronchodilator effect of atrial natriuretic peptide on asthma. Br Med J. 1989;299:1081–2.
Hulks G, Jardine AG, Connell JMC, Thomson NC. Effect of atrial natriuretic factor on bronchomotor tone in the normal human airway. Clin Sci. 1990;79:51–5.
Hulks G, Jardine AG, Connell JMC, Thomson NC. Influence of elevated plasma levels of atrial natriuretic factor on bronchial reactivity in asthma. Am Rev Respir Dis. 1991;143:778–82.
McAlpine LG, Hulks G. Thomson NC. Effect of atrial natriuretic peptide given by intravenous infusion on bronchoconstriction induced by ultrasonically nebulised distilled water (FOG). Am Rev Respir Dis. 1992;146:912–15.
Angus RM, McCallum MJA, Hulks J, Thomson NC. Bronchodilator, cardiovascular and cyclic guanylyl monophosphate response to high-dose infused atrial natriuretic peptide in asthma. Am Rev Respir Dis. 1993;147:1122–5.
Hulks G, Thomson NC. High dose inhaled atrial natriuretic factor is a bronchodilator in asthmatic subjects (Abstract). Eur Respir J. 1991;4 (Suppl. 14):607s.
Hulks G, Thomson NC. Inhaled atrial natriuretic factor inhibits histamine induced bronchonstriction (Abstract). Eur Respir J. 1991;4 (Suppl. 14):606s-7s.
Hulks G, Thomson NC. Inhaled atrial natriuretic peptide in asthmatic airways. Br Med J. 1992;304:1156.
Angus RM, McCallum MJA, Thomson NC. Effect of inhaled atrial natriuretic peptide on methacholine induced bronchoconstriction in asthma. Clin Exp Allergy. 1994;24:784–8.
Flüge T, Wagner TOF, Fabel H, von Kortzfleisch V, von Eickstedt K-W. Dose-dependent bronchodilation of urodilatin in asthmatic subjects in vivo. Naunyn-Schmiedeberg’s Arch Pharmacol. 1992;345 Suppl. 2:R50.
Flüge T, Fabel H, Wagner TOF, Schneider B, Forssmann WG. Bronchodilating effect of natriuretic and vasorelaxant peptides compared to salbutamol in asthmatics. Regul Pept. 1995;59(3):357–70.
Flüge T, Fabel H, Wagner TOF, Schneider B, Forssmann WG. Urodilatin (Ularitide, INN): a potent bronchodilator in asthmatic subjects. Eur J Clin Invest. 1995;25:728–36.
Takagi K, Araki N. Relaxant effect of brain natriuretic peptide on guinea-pig tracheal smooth muscle. Clin Exp Pharmacol Physiol. 1993;20:239–43.
Watanabe H, Furui H, Yamaki K, Suzuki R, Takagi T, Satake T. Atrial natriuretic polypeptide causes dose-dependent relaxant effect on guinea pig tracheal smooth muscle. Am Rev Respir Dis. 1988;137:102.
Hutson PA, Church MK, Clay TP, Miller P, Holgate ST. Early and late-phase bronchoconstriction after allergen challenge of nonanesthetized guinea pigs. Am Rev Respir Dis. 1988;137:548–57.
Pennock BE, Cox CP, Rogers RM, Cain WA, Wells JH. A noninvasive technique for measurement of changes in specific airway resistance. J Appl Physiol Resp Environ Exercise Physiol. 1979;46(2):399–406.
Gros C, Souque A, Schwartz JC. Degradation of atrial natriuretic factor in mouse blood in vitro and in vivo: role of enkephalinase (EC 3.4.24.11). Neuropeptides. 1990;17:1–5.
Katsube N, Schwartz D, Needleman P. Atriopeptin turnover: quantitative relationship between in vivo changes in plasma levels and atrial content. J Pharmacol Exp Ther. 1986;239:474–9.
Luft F, Lang RE, Aronoff GR et al. Atriopeptin III kinetics and pharmacodynamics in normal and anephric rats. J Pharmacol Exp Ther. 1986;236:416–18.
Murthy KK, Thibault G, Garcia R, Gutkowska J, Genest J, Cantin M. Degradation of atrial natriuretic factor in the rat. Biochem J. 1986;240:461–9.
Murthy KK, Thibault G, Schiffrin EL et al. Disappearance of atrial natriuretic factor from circulation in the rat. Peptides. 1986;7:241–6.
Gnädinger MP, Lang RE, Hasler L, Uehlinger DE, Shaw S, Weidmann P. Plasma kinetics of synthetic alpha-human atrial natriuretic peptide in man. Miner Electrolyte Metab. 1986;12:371–4.
Jüppner H, Brabant O, Kapteine U, Kirschner M, Klein H, Hesch RD. Direct radioimmunoassay for human atrial natriuretic peptide (hANP) and its clinical evaluation. Biochem Biophys Res Commun. 1986;139:1215–23.
Nakao K, Sugawara A, Morii N et al. The pharmacokinetics of alpha-human atrial natriuretic peptide in healthy subjects. Eur J Clin Pharmacol. 1986;31:101–3.
Yandle TG, Richards AM, Nichollis MG, Cuneo R, Espiner EA, Livesey JH. Metabolic clearance rate and plasma half life of alpha-human atrial natriuretic peptide in man. Life Sci. 1986;38:1827–33.
Sachs L. Angewandte Statistik. Anwendung statistischer Methoden. Berlin, Heidelberg: Springer Verlag; 1992;401–13.
Amdur MO, Mead J. Mechanics of respiration in unanesthetized guinea pigs. Am J Physiol. 1985;192(2):364–8.
Holroyde MC, Norris AA. The effect of ozone on reactivity of upper and lower airways in guinea pigs. Br J Pharmacol. 1988;94:938–46.
Cotes JE. Lung Function: Assessment and Application in Medicine, 4th edn. London: Blackwell Scientific Publications; 1979:81.
Tenney SM, Remmers JE. Comparative quantitative morphology of the mammalian lung diffusing area. Nature. 1963;197:54–7.
Forrest JB, Weibel ER. Morphometric estimation of pulmonary diffusion capacity. VII. The normal guinea pig lung. Respir Physiol. 1975;24(2);191–202.
Baumal R, Hooi C, McAvoy D, Broder I. Time course of morphometric changes after acute allergic bronchoconstriction in the guinea pig. Int Arch Allergy Appl Immunol. 1983;71(2):131–6.
Takezawa J, Miller FJ, O’Neil JJ. Single breath diffusing capacity and lung volume in small laboratory animals. J Appl Physiol. 1980;48(6):1052–9.
Pare PD, Michoud MC, Boucher RC, Hogg JC. Pulmonary effects of acute and chronic antigen exposure of immunized guinea pigs. J Appl Physiol. 1979;46:346–53.
Lamm WJE, Hildebrandt JR, Hildebrandt J, Lai YL. End-expiratory volume in the rat: effects of consciousness and body position. J Appl Physiol. 1982;53(5):1071–9.
Agrawal KP. Specific airway conductance in guinea pigs: normal values and histamine induced fall. Resp Physiol. 1981;43:23–30.
Stein M, Schiavi RC, Ottenberg P, Hamilton C. The mechanical properties of the lungs in experimental asthma in the guinea pig. J Allergy. 1961;32(1):8–16.
Mead J. Control of respiratory frequency. J Appl Physiol. 1960;15:325–36.
Douglas JS, Dennis MW, Ridgway P, Bouhuys A. Airway dilatation and constriction in spontaneously breathing guinea pigs. J Pharmacol Exp Ther. 1972;180(1):98–109.
Manning PJ, Jones GL, O’Byrne PM. Tachyphylaxis to inhaled histamine in asthmatic subjects. J Appl Physiol. 1987;63:1572–7.
Manning PJ, O’Byrne PM. Histamine bronchoconstriction reduces airway responsiveness in asthmatic subjects. Am Rev Resp Dis. 1988;137:1323–5.
Connolly MJ, Hendrick DJ. Refractory period following histamine provoked bronchospasm in asthmatic subjects. Thorax. 1985;40:216–17.
Candenas M-L, Naline E, Puybasset L, Devillier P, Advenier C. Effect of atrial natriuretic peptide and of atriopeptins on the human isolated bronchus. Comparison with the guinea-pig isolated trachea. Pulmon Pharmacol. 1991;4:120–5.
Abdallah A, Burnell J, Pelton T, Berman J Bronchodilator activity of synthetic atrial natriuretic factor (rANF-27);comparison with aminophylline and salbutamol. Fed Proc. 1986;M45(1):203.
Hamel R, Ford-Hutchinson AW. Relaxant profile of synthetic atrial natriuretic factor on guinea-pig pulmonary tissues. Eur J Pharmacol. 1986;121:151–5.
Watanabe H, Takagi K, Satake T. Relaxant effects of atrial natriuretic polypeptide on guinea pig tracheal smooth muscle. Prog Biochem Pharmacol. 1988;23:136–41.
Duft S, Marxen P, Deeb M et al. Urodilatin and atrial natriuretic factor relax preconstricted isolated perfused guinea pig trachea and increase cyclic GMP. Naunyn-Schmiedeberg’s Arch Pharmacol. 1992;345(Suppl. 2):R49.
Grunstein MM, Tanaka DT, Knight MS. Maturation of histamine reactivity in the isolated rabbit airway (abstract). Am Rev Respir Dis. 1986;133:A113.
Fleisch JH, Maling AM, Brodie BB. Evidence for the existence of alpha-adrenergic receptors in the mammalian trachea. Am J Physiol. 1970:218:596–9.
Gagelmann M, Hock D, Forssmann W-G. Urodilatin (CCD/ANP-95-126) is not biologically inactivated by a peptidase from dog kidney cortex membranes in contrast to atrial natriuretic peptide/cardiodilatin (alpha-hANP/CDD-99-126). FEBS Lett. 1988;233:249–54.
Heim J-M, Kiefersauer S, Fülle H-J, Gerzer R. Urodilatin and β-ANF: binding properties and activation of particulate guanylate cyclase. Biochem Biophys Res Commun. 1989;163(1):37–41.
Flüge T, Fabel H, Wagner TOF, Forssmann WG. Localization of the bronchodilator effect induced by type A natriuretic peptide in asthmatic subjects. Clin Invest. 1994;72:772–4.
Suga S-I, Nakao K, Hosoda K et al. Receptor selectivity of natriuretic peptide family, atrial natriuretic peptide, brain natriuretic peptide and C-type natriuretic peptide. Endocrinology. 1992;130:229–39.
Zhou H-L, Fiscus RR. Brain natriuretic peptide (BNP) causes endothelium-independent relaxation and elevation of cyclic GMP in rat thoracic aorta. Neuropeptides. 1989;14:161–9.
Nakao K, Mukoyama M, Hosoda K et al. Biosynthesis, secretion, and receptor selectivity of human brain natriuretic peptide. Can J Physiol Pharmacol. 1991;69:1500–6.
Takagi K, Araki N, Suzuki K. Relaxant effect of C-type natriuretic peptide on guinea pig tracheal smooth muscle. Arzneim-Forsch./Drug Res. 1992;42(II):1329–31.
Kita T, Kida O, Yokota N et al. Effects of brain natriuretic peptide-45, a circulating form of rat brain natriuretic peptide, in spontaneously hypertensive rats. Eur J Pharmacol. 1991;202:73–9.
Mukoyama M, Nakao K, Hosoda K et al. Brain natriuretic peptide as a novel cardiac hormone in humans. Evidence for an exquisite dual natriuretic peptide system, atrial natriuretic peptide and brain natriuretic peptide. J Clin Invest. 1991;87:1402–12.
Maack T, Suzuki M, Almeida FA et al. Physiological role of silent receptors of atrial natriuretic factor. Science. 1987;238:675–8.
Fuller F, Porter JG, Arfsten AE et al. Atrial natriuretic peptide clearance receptor. Complete sequence and functional expression of cDNA clones. J Biol Chem. 1988;263:9395–401.
Porter JG, Wang Y, Schwartz K et al. Characterization of the atrial natriuretic peptide clearance receptor using a vaccinia virus expression vector. J Biol Chem. 1988;263(35):18827–33.
Almeida FA, Suzuki M, Scarborough RM, Lewicki JA, Maack T. Clearance function of type C receptors of atrial natriuretic factor in rats. Am J Physiol. 1989;256:R469–75.
Michoud M-C, Fraser RS, Pare PD, Hogg JC. Effect of indomethacin and atropine in experimental asthma in conscious guinea pigs. J Appl Physiol. 1976;40(6):889–94.
Mills JE, Widdicombe JG. Role of the vagus nerves in anaphylaxis and histamine-induced bronchoconstriction in guinea pigs. Br J Pharmacol. 1976;39:724–31.
Author information
Authors and Affiliations
Additional information
This work is a short version in English of the doctoral thesis submitted to the Medical Faculty of the RWTH Aachen
Rights and permissions
About this article
Cite this article
Hermel, M. Influence of atrial natriuretic peptide, brain natriuretic peptide and urodilatin on the histamine-induced bronchoconstriction in the conscious guinea pig. Inflammopharmacol 6, 159–178 (1998). https://doi.org/10.1007/s10787-998-0032-2
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10787-998-0032-2