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Cellular pathology of experimental colitis induced by trinitrobenzenesulphonic acid (TNBS): Protective effects of recombinant human interleukin-11

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Abstract

The objective of this study was to elucidate colonic mucosal ultrastructural effects of trinitrobenzene-sulphonic acid (TNBS) with and without co-administration of recombinant human interleukin-11 (rhIL-11). Using a standard colitis model (ir alcoholic TNBS), rats were sacrificed at 3~14 days after TNBS. Co-administration of rhIL-11 (100, 300 or 1000 μg/kg sc) was given for protection, and controls received saline or alcohol ir, or rhIL-11 sc alone. Transmission electron microscopy revealed that early TNBS-induced cytopathology was primarily in absorptive cells, changes which occurred prior to goblet cell damage. Progressive cellular changes included vacuolization and increased multivesicular bodies in cell apices, disconfiguration of microvilli, enlarged Golgi apparatuses, enlargement of basal inter-cellular spaces, and eventual desquamation of epithelium and apical bursting.

Organelle damage preceded surface changes and resembled ultrastructural changes reported for human ulcerative colitis. The principal effect of rhIL-11 was apparent massive release of mucus from goblet cells, filling the colonic crypts, and suggesting a mode of its protection.

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Authors and Affiliations

  1. Department of Biomedical Sciences and Pathobiology, VMRCVM, Virginia Polytechnic Institute and State University, 24061, Blacksburg, VA, USA

    C. J. Pfeiffer

  2. Department of Hygiene and International Center for Medical Research (ICMR), Kobe University School of Medicine, Kusunoki-cho, 7-chome, 650, Kobe, Japan

    S. Sato

  3. Division of Gastroenterology, McMaster University Faculty of Medicine, 1200 Main Street West, L8N 325, Hamilton, Ontario, Canada

    B. S. Qiu

  4. Division of Preclinical Biology, Genetics Institute, Inc., 1 Burtt Road, 01810, Andover, MA, USA

    J. C. Keith

  5. Department of Pharmacology, A. Menarini Pharmaceuticals, Via Sette Santi 3, 50131, Florence, Italy

    S. Evangelista

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  1. C. J. Pfeiffer
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  2. S. Sato
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  3. B. S. Qiu
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  4. J. C. Keith
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  5. S. Evangelista
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Pfeiffer, C.J., Sato, S., Qiu, B.S. et al. Cellular pathology of experimental colitis induced by trinitrobenzenesulphonic acid (TNBS): Protective effects of recombinant human interleukin-11. Inflammopharmacol 5, 363–381 (1997). https://doi.org/10.1007/s10787-997-0033-6

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  • DOI: https://doi.org/10.1007/s10787-997-0033-6

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