Anti-inflammatory activity of a lipid fraction (lyprinol) from the NZ green-lipped mussel
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A lipid-rich extract, preparared by supercritical fluid extraction of fresh stabilized mussel powder (Lyprinol), showed significant anti-inflammatory (AI) activity given therapeutically and prophylactically po to Wistar and Dark Agouti rats developing either (a) adjuvant-induced polyarthritis or (b) collagen(II)-induced autoallergic arthritis, with ED50≤15 mg/kg; c.f. naproxen≥25 mg/kg or various therapeutic oils (flaxseed, evening primrose, fish)≥1800 mg/kg given orally. Lyprinol showed little or no activity in acute irritation assays (carrageenan, kaolin, histamine) indicating it is not mimicking rapid-acting NSAIDs.
Incorporating Lyprinol into arthritigenic adjuvants composed of heat-killed Mycobacterium. tuberculosis suspended in olive oil or squalane, effectively prevented arthritis development at a dose of 5 mg/rat. By contrast, ‘dummy adjuvants’ prepared with Mycobacterium tuberculosis and flaxseed, evening primrose or fish oils were still arthritigenic in Dark Agouti rats (doses of oil=90 mg/rat).
Lyprinol subfractions inhibited leukotriene-B4 biosynthesis by stimulated human polymorphonuclear leukocytes in vitro, and prostaglandin-E2 production by activated human macrophages in vitro. Much of this AI activity was associated with polyunsaturated fatty acids and natural antoxidants (carotenoids, etc.).
In contrast to NSAIDs, Lyprinol is non-gastrotoxic in disease-stressed rats at 300 mg/kg po and does not seem to affect platelet aggregation (human, rat). These data show Lyprinol to be a reproducible, relatively stable, source of bioactive lipids with much greater potency than plant/marine oils currently used as nutritional supplements to ameliorate signs of inflammation.
KeywordsNZ green-lipped mussel Lyprinol Lipid fraction Inflammation NSAIDs
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- 1.Croft JE. Relief from arthritis: a safe and effective treatment from the ocean. Wellingborough (UK): Thorsons Publishers; 1979:128.Google Scholar
- 2.Zwar D. The magic mussel—arthritis another way? 2nd edn. Cairns, Australia: Ideas Unlimited. 1994:108.Google Scholar
- 4.Gibson RD, Gibson SLM. Green-lipped mussel extract in arthritis. Lancet. 1981;i:149.Google Scholar
- 5.Gibson RD, Gibson SLM. Seatone in arthritis. Br Med J., 1981;283:1472.Google Scholar
- 6.Audeval B, Bouchacourt P. Etude controle en double aveugle contra placebo de l'extrait de moule Perna canaliculus dans la gonarthrose. Gaz Medicale. 1986;38:111–16.Google Scholar
- 7.Highton TC, McArthur AW. Pilot study on the effect of NZ green lipped mussel on rheumatoid arthritis. NZ Med J. 1975;80:261–3.Google Scholar
- 11.Couch RA, Ormrod DJ, Miller TE, Watkins WR. Anti-inflammatory activity in fractionated extracts of the green-lipped mussel. NZ Med J. 1982;95:803–6.Google Scholar
- 13.Rainsford KD, Whitehouse MW. Gastroprotective and anti-inflammatory properties of green-lipped mussel (Perna canaliculus) preparation. Arzneim-forsch. 1980;30:2128–32.Google Scholar
- 14.Cullen JC, Flint MJ, Leider J. The effects of dried mussel extract on an induced polyarthritis in rats. NZ Med J. 1975;81:260–1.Google Scholar
- 16.Miller T, Wu H. In vivo evidence for prostaglandin activity in New Zealand green-lipped mussel extract. NZ Med J. 1984;97:355–7.Google Scholar
- 17.Broadbent JM, Kosuge Y. Stabilised mussel extract. NZ Patent 211928 (29 April 1985); Australian patent PG 4775/84 (1 May 1984).Google Scholar
- 18.Macrides T, Kalafatis N. Lipid extract having anti-inflammatory activity. Int. Patent application PCT/AU 96/00564.Google Scholar
- 19.Macrides T, Kalafatis N. Anti-inflammatory preparation. Int. Patent application PCT/AU 95/00485.Google Scholar
- 20.Cremer M. Type II collagen-induced arthritis in rats. In: Greenwald RA, Diamond HS, eds. Handbook of Animal Models for the Rheumatic Diseases, Vol. 1. Boca Raton: CRC Press; 1988:17–27.Google Scholar
- 26.Macrides TA, Treschow AP, Kalafatis N, Wright PFA. The anti-inflammatory effects of Omega 3 tetraenoic fatty acids isolated from a lipid extract (Lyprinol) from the New Zealand, green-lipped mussel. Abstr. 88th American Oil Chemists Society Annual Meeting, Seattle, May, 1997.Google Scholar