Abstract
Purpose
To evaluate the effect of oral magnesium sulfate (MgSO4) on the gene expression and serum levels of inflammatory cytokines including TNF-α, IL-18, IL-1β, IL-6, and IFN-γ in patients with moderate coronary artery disease (CAD).
Methods
60 CAD patients were selected based on angiography findings and were randomly divided into two groups that received 300 mg/day MgSO4 (n = 30) or placebo (n = 30) for 3 months. Gene expression and serum levels of inflammatory cytokines were assessed.
Results
After 3 months of intervention, gene expression and serum levels of IL-18 and TNF-α in the MgSO4 group were significantly less than the placebo group (P < 0.05). However, no significant difference in gene expression and serum levels of IL-1β, IL-6, and IFN-γ was observed between the two groups (P > 0.05). In addition, within group analysis demonstrate that Mg-treatment significantly decrease serum level of TNF-α and IL-18 as compared to pretreatment.
Conclusion
The results of our study demonstrate that 3-month magnesium sulfate administration (300 mg/day) to CAD patients could significantly decrease serum concentration and gene expression levels of IL-18 and TNF-α. Our findings support the potential beneficial effect of magnesium supplementation on alleviating CAD complications through modulating inflammatory cytokines.
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Data availability
The data that support the findings of this study are available from the corresponding author, [Ebrahim Eftekhar], upon reasonable request.
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Funding
This work was supported by the [Molecular Medicine Research Center, Hormozgan University of Medical Sciences] under Grant numbers [970325 and 970362].
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EE: contributed to study design, supervised all stages of study and revised the manuscript. FM: methodology experimentation, wrote and modified the manuscript. MH; revised the manuscript and participated in methodology experimentation. SO, MSV, SK: participated in methodology experimentation. HF: participated in study design and data gathering. All authors approved the finally manuscript content.
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The study protocol was approved by the research ethics committee of Hormozgan University of Medical Sciences (IR.HUMS.REC.1397.331).
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All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national). Informed consent was obtained from all patients for being included in the study.
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Mohebi, F., Ostadhadi, S., Vaziri, M.s. et al. The effect of magnesium sulfate on gene expression and serum level of inflammatory cytokines in coronary artery disease patients. Inflammopharmacol 31, 2421–2430 (2023). https://doi.org/10.1007/s10787-023-01328-4
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DOI: https://doi.org/10.1007/s10787-023-01328-4