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Cytokine profile and antioxidants status in the moderate and severe COVID-19 patients: a trial of ozone therapy impact as a medicinal supplement

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Complementary ozone therapy has been identified as a revolutionary medical technique for a number of goals and ailments. At the present, it has been shown that ozone has medicinal qualities, such as antibacterial, antifungal, and antiparasitic properties. Coronavirus (SARS-CoV-2) is quickly spread over the globe. Cytokine storms and oxidative stress seem to play a substantial role in the most of acute attacks of the disease. The aim of this research was to assess the therapeutic advantages of complementary ozone therapy on the cytokine profile and antioxidant status in COVID-19 patients.


The statistical sample of this study included two hundred patients with COVID-19. One hundred COVID-19 patients (treatment group) received 240 ml of the patient's blood and an equal volume of O2/O3 gas at a concentration of 35–50 μg/ml daily, which gradually increased in concentration, and were kept for 5–10 days and one hundred patients (control group) received standard treatment. The secretion levels of IL-6, TNF-α, IL-1β, IL-10 cytokines, SOD, CAT and GPx were compared between control patients (standard treatment) and standard treatment plus intervention (ozone) before and after treatment.


The findings indicated a significant decrease in the level of IL-6, TNF-α, IL-1β in group receiving complementary ozone therapy in compared with control group. Furthermore, a significant increase was found in the level of IL-10 cytokine. Moreover, SOD, CAT and GPx levels revealed a significant increase in complementary ozone therapy group compared to control group.


Our results revealed that complementary ozone therapy can be used as a medicinal complementary therapy to reduce and control inflammatory cytokines and oxidative stress status in patients with COVID-19 as revealed its antioxidant and anti-inflammatory effects.

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The data sets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.


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The authors wish to thank all staff of Baqiyatallah Hospital (Tehran, Iran) and Ozone research center of Baqiyatallah university of medical science and Seyed Alireza Mirilavasani, and Ali Asghar Akhlaghi for their cooperation in implementing experimental procedures and analysis of data.


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All authors contributed substantially in the design, implementation of the study and data collection, and wrote the first draft of the manuscript and performed statistical analysis. All authors read and approved the final manuscript.

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Correspondence to Morteza Izadi or Mohammad Javanbakht.

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The authors declare that they have no conflict of interest.

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This study was conducted in compliance with the declaration of Helsinki. The study was approved by the Institutional Review Board of, Baqiyatallah University of Medical Sciences.

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Ghaleh, H.E.G., Izadi, M., Javanbakht, M. et al. Cytokine profile and antioxidants status in the moderate and severe COVID-19 patients: a trial of ozone therapy impact as a medicinal supplement. Inflammopharmacol 31, 3029–3036 (2023).

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