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Zygophyllum album leaves extract prevented hepatic fibrosis in rats, by reducing liver injury and suppressing oxidative stress, inflammation, apoptosis and the TGF-β1/Smads signaling pathways. Exploring of bioactive compounds using HPLC–DAD–ESI–QTOF-MS/MS

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Abstract

Zygophyllum album is traditionally used against many illnesses, such as liver disease. The present study investigated the bioactive compounds in methanol extract of Z. album (MEZA) using HPLC–DAD–ESI–QTOF-MS/MS and explored its possible antioxidative, anti-inflammatory, anti-apoptotic, and hepatoprotective effect. Twelve phenolic compounds were identified; isorhamnetin-3-O-rutinoside being the main one was the main composite (144.6 mg/100 g dm). Results showed that MEZA reduced significantly the biochemical markers (AST, ALT, LDH and ALP), and the hepatic oxidative stress indicators (MDA, PC, SOD, CAT, and GPx) in deltamethrin (DLM)-treated rats. Moreover, MEZA limited the inflammatory responses through downregulation of NF-κB gene, which suppressed the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6). Furthermore, Z. album reduced DLM-induced apoptosis by attenuating caspase 3 and p53 mRNA activation. MEZA treatment also alleviated upregulation of α-SMA, type I collagen, and TGF-β1 mRNA in the liver. The possible antifibrotic effect of MEZA was clearly demonstrated by the histopathology examination, using Masson’s Trichrome and Sirius Red stainings. Therefore, the current study suggested that the bioactive compounds of Z. album possessed antifibrotic effect against DLM-induced hepatic fibrosis, by protecting liver tissue, and inhibiting oxidative stress, inflammation, apoptosis and the TGF-β1/Smads signaling pathways.

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Acknowledgements

This work was supported by the Ministry of Higher Education and Scientific Research, Tunisia (Project number: 19PEJC06-04). This work was also supported by Researchers Supporting Project number RSP-2019/17, King Saud University, Riyadh, Saudi Arabia. Authors gratefully appreciate the facilities and the support provided by the Spanish Ministry of Economy and Competitiveness (AGL2015-67995-C3-2-R) for their help in carrying out the analytical part. The authors would like to express their sincere gratitude to the Tunisian Ministry of higher Education, and to Rached Raddadi, Hafedh Trabelsi, and Jihen Jeffel, technicians at Anatomopathology Laboratory, Gafsa, Tunisia, for their assistance in histological studies. M.d.M.C. thanks the support of the postdoctoral grant funded by the Acción 6 del Plan de Apoyo a la Investigación de la Universidad de Jaén, 2017–2019 and FEDER UJA project 1260905 funded by Programa Operativo FEDER 2C14-2020. And Ū Consejer a de Economa y Conocimiento de la Junta de Andaluc a.

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Authors and Affiliations

  1. Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, 2112, Gafsa, Tunisia

    Anouar Feriani, Lakhdar Ghazouani & Afoua Mufti

  2. Laboratoire D’Ecologie, de Biologie Et de Physiologie Des Organismes Aquatiques, LR18ES41, Faculté Des Sciences de Tunis, Université Tunis EL Manar, 2092, Tunis, Tunisia

    Meriam Tir

  3. Department of Analytical Chemistry, University of Granada, Avda. Fuentenueva S/N, 18071, Granada, Spain

    Ana María Gómez-Caravaca, María del Mar Contreras & Antonio Segura-Carretero

  4. Department of Chemical, Environmental and Material Engineering, University of Jaén, Campus Las Lagunillas, s/n, 23071, Jaén, Spain

    María del Mar Contreras

  5. Laboratory of Olive Biotechnology, Center of Biotechnology of Borj-Cédria, BP. 901, 2050, Hammam-Lif, Tunisia

    Amani Taamalli

  6. Institut Supérieur des Sciences et Technologies de l’Environnement, ISSTE de Borj Cédria, Université de Carthage, 2050, Hammam-Lif, Tunisia

    Nizar Tlili

  7. Faculté Des Sciences de Tunis (UR13/ES25), Université Tunis El-Manar, 2092, Tunis, Tunisia

    Nizar Tlili

  8. Laboratory of Animal Ecophysiology, Faculty of Science of Sfax, 3018, Sfax, Tunisia

    Abdelfattah El Feki & Mohamed Salah Allagui

  9. Department of Zoology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia

    Abdel Halim Harrath

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Feriani, A., Tir, M., Gómez-Caravaca, A.M. et al. Zygophyllum album leaves extract prevented hepatic fibrosis in rats, by reducing liver injury and suppressing oxidative stress, inflammation, apoptosis and the TGF-β1/Smads signaling pathways. Exploring of bioactive compounds using HPLC–DAD–ESI–QTOF-MS/MS. Inflammopharmacol 28, 1735–1750 (2020). https://doi.org/10.1007/s10787-020-00700-y

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