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Etlingera pavieana extract attenuates TNF-α induced vascular adhesion molecule expression in human endothelial cells through NF-κB and Akt/JNK pathways

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Abstract

The aim of this study was to determine whether ethanol extracts of Etlingera pavieana rhizomes (EPE) can inhibit the expression of ICAM-1 and VCAM-1 in TNF-α-stimulated human vascular endothelial cells. EPE significantly reduced ICAM-1 and VCAM-1 expression in a concentration-dependent manner. EPE also suppressed phospho-IκB level and nuclear translocation of NF-κB. EPE significantly inhibited phosphorylation of JNK and c-Jun, a major component of AP-1, but had no effects on ERK and p38 MAPK pathways. Akt phosphorylation was increased in the presence of EPE, and wortmannin and SP600125 reversed the inhibitory effects of EPE on ICAM-1 and VCAM-1 expression. Furthermore, the active EPE constituents 4-methoxycinnamyl p-coumarate and trans-4-methoxycinnamaldehyde attenuated TNF-α-induced expression of ICAM-1 and VCAM-1. Taken together, our data indicate that EPE protects against vascular inflammation in endothelial cells, in part via NF-κB and Akt/JNK signalings. In future studies, E. pavieana may be developed as a therapeutic agent or dietary supplement for treating and preventing inflammatory diseases.

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Abbreviations

Akt:

Protein kinase B

AP-1:

Activator protein-1

CVDs:

Cardiovascular diseases

ERK:

Extracellular signal-regulated kinase

H2DCF-DA:

2,7-Dichlorodihydrofluorescein diacetate

ICAM-1:

Intercellular adhesion molecule-1

IκBα:

Inhibitor of κBα

JNK:

C-Jun NH2-terminal kinase

MAPKs:

Mitogen-activated protein kinases

MCC:

4-methoxycinnamyl p-coumarate

MCD:

Trans-4-methoxycinnamaldehyde

MTT:

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

NF-κB:

Nuclear factor-kappa B

PI3K:

Phosphatidylinositol 3′-kinase

ROS:

Reactive oxygen species

TNF-α:

Tumor necrosis factor-α

VCAM-1:

Vascular cell adhesion molecule-1

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Acknowledgements

This work was financially supported by the Research Grant of Burapha University through National Research Council of Thailand (Grant no.76/2559) and for the Research Unit of Natural Bioactive Compounds for Healthcare Products Development. The authors would like to thank the Center of Excellence for Innovation in Chemistry (PERCH-CIC), Commission on Higher Education, Ministry of Education, Thailand and thank to Ms. Petchrat Sawai and Ms. Sakulrat Mankhong for valuable technical.

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Authors and Affiliations

  1. Department of Biochemistry and Research Unit of Natural Bioactive Compounds for Healthcare Products Development, Faculty of Science, Burapha University, Chonburi, 20131, Thailand

    Klaokwan Srisook, Kamonporn Potiprasart & Songklod Sarapusit

  2. Center of Excellence for Innovation in Chemistry, Faculty of Science, Burapha University, Chonburi, Thailand

    Klaokwan Srisook, Songklod Sarapusit & Ekaruth Srisook

  3. Department of Pharmacology, Hypoxia-Related Disease Research Center, College of Medicine, Inha Research Institute for Medical Sciences, Inha University, Incheon, 22212, Republic of Korea

    Chang-Shin Park

  4. Department of Chemistry and Research Unit of Natural Bioactive Compounds for Healthcare Products Development, Faculty of Science, Burapha University, Chonburi, Thailand

    Ekaruth Srisook

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  1. Klaokwan Srisook
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  2. Kamonporn Potiprasart
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Correspondence to Klaokwan Srisook.

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Srisook, K., Potiprasart, K., Sarapusit, S. et al. Etlingera pavieana extract attenuates TNF-α induced vascular adhesion molecule expression in human endothelial cells through NF-κB and Akt/JNK pathways. Inflammopharmacol 28, 1649–1662 (2020). https://doi.org/10.1007/s10787-019-00676-4

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  • DOI: https://doi.org/10.1007/s10787-019-00676-4

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