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Topical application of Mentha piperita essential oil accelerates wound healing in infected mice model

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Abstract

This study was conducted to evaluate the effects of the prepared ointments from Mentha piperita essential oil (M. piperita) on wound healing in the infected mice models. Each circular full-thickness wound was inoculated with 25 × 107 units of Staphylococcus aureus and Pseudomonas aeruginosa bacteria strains. The tissue bacterial count, histological analyses and expression levels of IL-10, TNF-α, TGF-β1, IL-1β, CCL2, CXCL1, VEGF and FGF-2 were assessed to identify the different doses of M. piperita on wound healing. Total tissue bacterial count, edema and inflammation level were declined, but the migration of fibroblasts, collagen synthesis and re-epithelization were increased in treated animals with M. piperita. The expression levels of CCL2, CXCL1, IL-1β, TGF-β1 and IL-10 genes were up-regulated in the M. piperita-treated animals compared to the control group. While the expression of TNF-α, VEGF and FGF-2 was down-regulated in comparison to the control group. This study indicated that M. piperita can be used for treatment of the infected wound.

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References

  • Bielefeld KA, Amini-Nik S, Alman BA (2013) Cutaneous wound healing: recruiting developmental pathways for regeneration. Cell Mol Life Sci 6:1–23

    Google Scholar 

  • Campos M, Calixto J (2000) Neurokinin mediation of edema and inflammation. Neuropeptide 34(5):314–322

    Article  CAS  Google Scholar 

  • Çetin B, Kaya Y, Çakir A, Özer H, Aksakal Ö, Mete E (2016) Antimicrobial activities of essential oils and hexane extracts of two Turkish spice plants, cymbocarpum erythraeum (DC.) Boiss. and Echinophora tenuifolia L. against foodborne microorganisms. Rec Nat Prod 10(4):426–435

    Google Scholar 

  • Charo IF, Ransohoff RM (2006) The many roles of chemokines and chemokine receptors in inflammation. N Engl J Med 354:610–621

    Article  CAS  PubMed  Google Scholar 

  • Eo H, Lee H-J, Lim Y (2016) Ameliorative effect of dietary genistein on diabetes induced hyper-inflammation and oxidative stress during early stage of wound healing in alloxan induced diabetic mice. Biochem Biophys Res Commun 478:1021–1027

    Article  CAS  PubMed  Google Scholar 

  • Fang Y, Shen J, Yao M, Beagley K, Hambly B, Bao S (2010) Granulocyte-macrophage colony-stimulating factor enhances wound healing in diabetes via upregulation of proinflammatory cytokines. Br J Dermatol 162(3):478–486

    Article  CAS  PubMed  Google Scholar 

  • Farahpour MR, Mirzakhani N, Doostmohammadi J, Ebrahimzadeh M (2015) Hydroethanolic Pistacia atlantica hulls extract improved wound healing process; evidence for mast cells infiltration, angiogenesis and RNA stability. Int J Surg 17:88–98

    Article  PubMed  Google Scholar 

  • Farahpour MR, Vahid M, Oryan A (2017) Effectiveness of topical application of ostrich oil on the healing of Staphylococcus aureus-and Pseudomonas aeruginosa-infected wounds. Connect Tissue Res 31:1–10

    Article  CAS  Google Scholar 

  • Gillitzer R, Goebeler M (2001) Chemokines in cutaneous wound healing. J Leukoc Biol 69(4):513–521

    CAS  PubMed  Google Scholar 

  • Godwin JW, Rosenthal N (2014) Scar-free wound healing and regeneration in amphibians: immunological influences on regenerative success. Differentiation 87(1):66–75

    Article  CAS  PubMed  Google Scholar 

  • Herro E, Jacob SE (2010) Mentha piperita (peppermint). Dermatitis 21(6):327–329

    PubMed  Google Scholar 

  • Hozzeina WN, Badrc G, Al Ghamdid A, Sayede A, Al-Wailif NS, Garraudg O (2015) Topical application of propolis enhances cutaneous wound healing by promoting TGF-Beta/smad-mediated collagen production in a streptozotocin-induced type I diabetic mouse model. Cell Physiol Biochem 37:940–954

    Article  CAS  Google Scholar 

  • Jianu C, Pop G, Gruia AT, Horhat FG (2013) Chemical composition and antimicrobial activity of essential oils of lavender (Lavandula angustifolia) and lavandin (Lavandula × intermedia) grown in Western Romania. Int J Agric Biol 15(4):772–776

    CAS  Google Scholar 

  • Kimmel AR, Brasaemle DL, McAndrews-Hill M, Sztalryd C, Londos C (2010) Adoption of PERILIPIN as a unifying nomenclature for the mammalian PAT-family of intracellular lipid storage droplet proteins. J Lipid Res 51:468–471

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lauer G, Sollberg S, Cole M, Flamme I, Stürzebecher J, Mann K, Krieg T, Eming SA (2000) Expression and proteolysis of vascular endothelial growth factor is increased in chronic wounds. J Invest Dermatol 115:12–18

    Article  CAS  PubMed  Google Scholar 

  • Liang R, Xu S, Shoemaker CF, Li Y, Zhong F, Huang Q (2012) Physical and antimicrobial properties of peppermint oil nanoemulsions. J Agric Food Chem 60:7548–7755

    Article  CAS  PubMed  Google Scholar 

  • Lucas T, Waisman A, Ranjan R, Roes J, Krieg T, Müller W et al (2010) Differential roles of macrophages in diverse phases of skin repair. J Immunol 184(7):3964–3977

    Article  CAS  PubMed  Google Scholar 

  • Milatovic S, Nanney LB, Yu Y, White JR, Richmond A (2003) Impaired healing of nitrogen mustard wounds in CXCR2 null mice. Wound Repair Regen 11(3):213–219

    Article  PubMed  PubMed Central  Google Scholar 

  • Nathan C (2006) Neutrophils and immunity: challenges and opportunities. Nat Rev Immunol 6(3):173–182

    Article  CAS  PubMed  Google Scholar 

  • Okuda K, Murata M, Sugimoto M, Saito Y, Kabasawa Y, Yoshie H, Saku T, Hara K (1998) TGF‐β1 influences early gingival wound healing in rats: an immunohistochemical evaluation of stromal remodelling by extracellular matrix molecules and PCNA. J Oral Pathol Med 27:463–469

    Article  CAS  PubMed  Google Scholar 

  • Oryan A, Moshiri A (2011) A long term study on the role of exogenous human recombinant basic fibroblast growth factor on the superficial digital flexor tendon healing in rabbits. J Musculoskelet Neuron Interact 11(2):185–195

    CAS  Google Scholar 

  • Raman D, Sobolik-Delmaire T, Richmond A (2011) Chemokines in health and disease. Exp Cell Res 317(5):575–589

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Satish L (2015) Chemokines as therapeutic targets to improve healing efficiency of chronic wounds. Adv Wound Care 4(11):651–659

    Article  Google Scholar 

  • Sato Y, Ohshima T, Kondo T (1999) Regulatory role of endogenous interleukin-10 in cutaneous inflammatory response of murine wound healing. Biochem Biophys Res Commun 265(1):194–199

    Article  CAS  PubMed  Google Scholar 

  • Schmid P, Cox D, Bilbe G, McMaster G, Morrison C, Stähelin H, Lüscher N, Seiler W (1993) TGF-betas and TGF-beta type II receptor in human epidermis: differential expression in acute and chronic skin wounds. J Pathol 171:191–197

    Article  CAS  PubMed  Google Scholar 

  • Schultz GS, Wysocki A (2009) Interactions between extracellular matrix and growth factors in wound healing. Wound Repair Regen 17:153–162

    Article  PubMed  Google Scholar 

  • Shalayel MHF, Asaad AM, Qureshi MA, Elhussein AB (2017) Anti-bacterial activity of peppermint (Mentha piperita) extracts against some emerging multi-drug resistant human bacterial pathogens. J Herb Med 7:27–30

    Article  Google Scholar 

  • Sun Z, Wang H, Wang J, Zhou L, Yang P (2014) Chemical composition and anti-inflammatory, cytotoxic and antioxidant activities of essential oil from leaves of Mentha piperita grown in China. PLoS One 9(12):e114767

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgement

This study was extracted from DVM thesis of Mr Mohammad Moddaresi in Veterinary Faculty of Islamic Azad University, Urmia Branch. The authors are grateful to Dr. Farahnaz Tahery for ointment formulation and Dr. Sheryl Thomas for the native language edition.

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Authors and Affiliations

  1. Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran

    Mohammad Modarresi

  2. Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, 57159-44867, Iran

    Mohammad-Reza Farahpour

  3. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Behzad Baradaran

Authors
  1. Mohammad Modarresi
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  2. Mohammad-Reza Farahpour
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  3. Behzad Baradaran
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Correspondence to Mohammad-Reza Farahpour.

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Modarresi, M., Farahpour, MR. & Baradaran, B. Topical application of Mentha piperita essential oil accelerates wound healing in infected mice model. Inflammopharmacol 27, 531–537 (2019). https://doi.org/10.1007/s10787-018-0510-0

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  • DOI: https://doi.org/10.1007/s10787-018-0510-0

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