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Effect of Synadenium grantii and its isolated compound on dysmenorrhea behavior model in mice

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Abstract

Objective

This study aimed to evaluate the effects of the methanol extract of Synadenium grantii Hook f. stems and its main isolated compound Query3,4,12,13-tetraacetylforbol-20-phenylacetate (1) on experimental dysmenorrhea in mice.

Methods

Female Swiss mice (n = 6–15) of 6–8 weeks old were used, selected according to the period of the estrous cycle. Animals in proestrus were treated intraperitoneally (i.p.) for 3 days with estradiol. They orally received, on the 4th day, S. grantii extract, the compound 1, ibuprofen or hyoscine butylbromide (Buscopan compound®). Then they were injected (i.p.) with oxytocin 1 h later and individually observed regarding the abdominal writhing for 45 min. The uterus was weighed, photographed and fixed in paraffin for histological analysis.

Key findings

The extract inhibited the abdominal writhing and similar results were obtained with compound 1 and the positive control drugs Ibuprofen and hyoscine butylbromide. Reduction of uterus volume and histological inflammatory parameters, such as oedema and leukocyte infiltrate, were observed in animals treated with the extract and compound 1.

Conclusions

Our data show promising activity of the extract against dysmenorrhea, indicating important anti-inflammatory activity. Compound 1 appears to be, at least in part, the main responsible for this promising biological effect.

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Acknowledgements

This work was supported by Grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and the Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina (FAPESC).

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Authors and Affiliations

  1. Posgraduate Program in Pharmaceutical Science, CCS, Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, n° 458, Bloco F6, CCS, sala 301, Itajaí, SC, CEP 88302-901, Brazil

    Flávia Werner da Rocha Jesuíno, Adriana Campos, Valdir Cechinel Filho & Nara Lins Meira Quintão

  2. Biology Course, CCS, Universidade do Vale do Itajaí (UNIVALI), Itajaí, Brazil

    Jaqueline Pavesi Reis & Maria Verônica Dávila Pastor

  3. Biomedicine Course, CCS, Universidade do Vale do Itajaí (UNIVALI), Itajaí, Brazil

    Juliana Cristina Pereira Whitaker

  4. Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), CCS, Universidade do Vale do Itajaí (UNIVALI), Itajaí, Brazil

    Valdir Cechinel Filho

Authors
  1. Flávia Werner da Rocha Jesuíno
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  2. Jaqueline Pavesi Reis
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  3. Juliana Cristina Pereira Whitaker
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  4. Adriana Campos
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  5. Maria Verônica Dávila Pastor
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  6. Valdir Cechinel Filho
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  7. Nara Lins Meira Quintão
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Correspondence to Nara Lins Meira Quintão.

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Jesuíno, F.W.d., Reis, J.P., Whitaker, J.C.P. et al. Effect of Synadenium grantii and its isolated compound on dysmenorrhea behavior model in mice. Inflammopharmacol 27, 613–620 (2019). https://doi.org/10.1007/s10787-018-0501-1

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  • DOI: https://doi.org/10.1007/s10787-018-0501-1

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