Propolis modulates NOS2/arginase-1 pathway in tropomyosin-induced experimental autoimmune uveitis

Abstract

In this study, we evaluated the preventive and curative effects of ethanolic extract of Propolis (EEP) during α-Tropomyosin-induced uveitis in an experimental model using Wistar rats, through the regulation of inducible nitric oxide synthase (NOS2) and arginase-1. In this context, rats received daily injection of EEP (100 mg/kg) for 5 days prior to immunization or for 9 days commencing 5 days post immunization with α-Tropomyosin extract, then were sacrificed at day 14. Histological examination, NOS2, arginase-1, and nuclear factor-κB (NF-κB) expression were evaluated in the retinas. Plasmatic production of nitric oxide (NO), urea, IL-4, and TNF-α was assessed. We have found that treatment with EEP substantially reduced the retinal histological damages induced by α-Tropomyosin. In the same context, a significant decrease of NO and TNF-α levels was noticed. Interestingly, EEP down-modulated NOS2 and NF-κB expression in retina. Also, an increase in urea and IL-4 levels was concomitant to an up-modulation of arginase-1 expression. Hence, it appears that EEP attenuated retinal damages through the induction of Th2 response and the inhibition of NF-κB/NOS2 pathway.

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Acknowledgements

The authors would like to thank the beekeeper Mohamed Kacioui for the generous gift of crude Propolis. Also, Fayçal Medjeber and Dalila Laid (Spectrol Lab. Algeria) for their technical assistance.

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Correspondence to Chafia Touil-Boukoffa.

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Touri, K., Belguendouz, H., Medjeber, O. et al. Propolis modulates NOS2/arginase-1 pathway in tropomyosin-induced experimental autoimmune uveitis. Inflammopharmacol 26, 1293–1303 (2018). https://doi.org/10.1007/s10787-018-0487-8

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Keywords

  • Propolis
  • α-Tropomyosin
  • Uveitis
  • NOS2
  • Arginase-1
  • Immuno modulation