, Volume 26, Issue 5, pp 1305–1316 | Cite as

Gastrodin microinjection suppresses 6-OHDA-induced motor impairments in parkinsonian rats: insights into oxidative balance and microglial activation in SNc

  • Rasool Haddadi
  • Maryam Poursina
  • Fatemeh Zeraati
  • Forough Nadi
Original Article


Purpose of the research

In this study, we appraised the effect of pre-treatment with intra-cerebro ventricular (i.c.v) microinjection of gastrodin (Gst) on catalepsy, motor imbalance, substantia nigra pars compacta (SNc) myeloperoxidase (MPO) activity, lipid peroxidation levels, nitric oxide (NO) production and total antioxidant capacity (TAC) in 6-hydroxydopamine (6-OHDA) rats model of PD.

Materials and methods

Male Wistar rats were pre-treated with i.c.v microinjections of Gst (20, 40 and 80 μg/3 μl/rat) for five consecutive days. Then, catalepsy and motor balance were induced by unilateral infusion of 6-OHDA (8 μg/2 μl/rat) into the SNc. The anti-cataleptic and motor balance improving effect of Gst was assessed by the Bar test and Rotarod 3 weeks after neurotoxin injection, respectively. SNc MPO activity and lipid peroxidation levels, NO production and TAC were assessed at the end of behavioral experiments.


Our data demonstrated that Gst pre-treatment significantly (p < 0.001) was prevented motor in-coordination and catalepsy in neurotoxin lesioned rats. The most motor improving effect was seen at 80 μg Gst (p < 0.001). Pre-treatment of parkinsonian rats with Gst meaningfully (p < 0.001) was suppressed MPO activity, lipid peroxidation and NO production. Furthermore, the TAC level in the SNc was increased (p < 0.001) in Gst-microinjected rats about to the normal non-parkinsonian animals.

Major conclusions

In summary, pre-treatment with Gst abolished 6-OHDA-induced catalepsy and improved motor incoordination by decreasing: SNc MPO activity, lipid peroxidation levels and NO production, and restoring SNc levels of TAC to the levels of healthy rats.

Graphical abstract


Gastrodin Catalepsy Rotarod Myeloperoxidase MDA Parkinson disease Rat 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interest.


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Rasool Haddadi
    • 1
    • 2
  • Maryam Poursina
    • 3
  • Fatemeh Zeraati
    • 1
    • 2
  • Forough Nadi
    • 3
  1. 1.Department of Pharmacology and Toxicology, School of PharmacyHamadan University of Medical SciencesHamadanIran
  2. 2.Herbal Medicine and Natural Product Research CenterHamadan University of Medical SciencesHamadanIran
  3. 3.Students Research CenterHamadan University of Medical SciencesHamadanIran

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