, Volume 26, Issue 4, pp 983–991 | Cite as

Plumbagin, a vitamin K3 analogue ameliorate malaria pathogenesis by inhibiting oxidative stress and inflammation

  • Amit Chand Gupta
  • Shilpa Mohanty
  • Archana Saxena
  • Anil Kumar Maurya
  • Dnyaneshwar U. Bawankule
Original Article


Plumbagin, a vitamin K3 analogue is the major active constituent in several plants including root of Plumbago indica Linn. This compound has been shown to exhibit a wide spectrum of pharmacological activities. The present investigation was to evaluate the ameliorative effects of plumbagin (PL) against severe malaria pathogenesis due to involvement of oxidative stress and inflammatory response in Plasmodium berghei infected malaria in mice. Malaria pathogenesis was induced by intra-peritoneal injection of P. berghei infected red blood cells into the Swiss albino mice. PL was administered orally at doses of 3, 10 and 30 mg/kg/day following Peter’s 4 day suppression test. Oral administration of PL showed significant reduction of parasitaemia and increase in mean survival time. PL treatment is also attributed to significant increase in the blood glucose and haemoglobin level when compared with vehicle-treated infected mice. Significant inhibition in level of oxidative stress and pro-inflammation related markers were observed in PL treated group. The trend of inhibition in oxidative stress markers level after oral treatment of PL was MPO > LPO > ROS in organ injury in P. berghei infected mice. This study showed that plumbagin is able to ameliorate malaria pathogenesis by augmenting anti-oxidative and anti-inflammatory mechanism apart from its effect on reducing parasitaemia and increasing mean survival time of malaria-induced mice.

Graphical Abstract


Plumbagin Malaria Oxidative stress Inflammation Mice 





Reactive oxygen species


Glutathione peroxidase


Superoxide dismutase


Tumour necrosis factor-alpha








Carboxymethyl cellulose


Thiobarbituric acid reactive substances








Hydrogen peroxide


2′,7′-Dichlorofluorescein diacetate






Foetal bovine serum




Phosphate-buffered saline


(3-(4,5-dimethylthiazol-2-yl)- 2,5-Diphenyltetrazolium)



The study was financially supported by the Council of Scientific and Industrial Research (CSIR), New Delhi under project BSC-0203 and HCP-0007. The authors are grateful to the UGC for providing fellowship to the first author and Director, CSIR-CIMAP, Lucknow, India for providing essential research facilities and support.

Compliance with ethical standards

Conflict of interest

Authors declare no conflicts of interest.


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Amit Chand Gupta
    • 1
  • Shilpa Mohanty
    • 1
  • Archana Saxena
    • 1
  • Anil Kumar Maurya
    • 1
  • Dnyaneshwar U. Bawankule
    • 1
  1. 1.In-Vivo Testing Laboratory, Molecular Bioprospection DepartmentCSIR-Central Institute of Medicinal and Aromatic PlantsLucknowIndia

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