Plumbagin, a vitamin K3 analogue ameliorate malaria pathogenesis by inhibiting oxidative stress and inflammation
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Plumbagin, a vitamin K3 analogue is the major active constituent in several plants including root of Plumbago indica Linn. This compound has been shown to exhibit a wide spectrum of pharmacological activities. The present investigation was to evaluate the ameliorative effects of plumbagin (PL) against severe malaria pathogenesis due to involvement of oxidative stress and inflammatory response in Plasmodium berghei infected malaria in mice. Malaria pathogenesis was induced by intra-peritoneal injection of P. berghei infected red blood cells into the Swiss albino mice. PL was administered orally at doses of 3, 10 and 30 mg/kg/day following Peter’s 4 day suppression test. Oral administration of PL showed significant reduction of parasitaemia and increase in mean survival time. PL treatment is also attributed to significant increase in the blood glucose and haemoglobin level when compared with vehicle-treated infected mice. Significant inhibition in level of oxidative stress and pro-inflammation related markers were observed in PL treated group. The trend of inhibition in oxidative stress markers level after oral treatment of PL was MPO > LPO > ROS in organ injury in P. berghei infected mice. This study showed that plumbagin is able to ameliorate malaria pathogenesis by augmenting anti-oxidative and anti-inflammatory mechanism apart from its effect on reducing parasitaemia and increasing mean survival time of malaria-induced mice.
KeywordsPlumbagin Malaria Oxidative stress Inflammation Mice
Reactive oxygen species
Tumour necrosis factor-alpha
Thiobarbituric acid reactive substances
Foetal bovine serum
The study was financially supported by the Council of Scientific and Industrial Research (CSIR), New Delhi under project BSC-0203 and HCP-0007. The authors are grateful to the UGC for providing fellowship to the first author and Director, CSIR-CIMAP, Lucknow, India for providing essential research facilities and support.
Compliance with ethical standards
Conflict of interest
Authors declare no conflicts of interest.
- Del Maestro R, McDonald W (1985) Oxidative enzymes in tissue homogenates. CRC Handb Methods Oxyg Rad Res 1:294–296Google Scholar
- Guha M, Kumar S, Choubey V, Maity P, Bandyopadhyay U (2006) Apoptosis in liver during malaria: role of oxidative stress and implication of mitochondrial pathway. FASEB J Off Publ Fed Am Soc Exp Biol 20:1224–1226Google Scholar
- Guiguemde WA, Hunt NH, Guo J, Marciano A, Haynes RK, Clark J, Guy RK, Golenser J (2014) Treatment of murine cerebral malaria by artemisone in combination with conventional antimalarial drugs: antiplasmodial effects and immune responses. Antimicrob Agents Chemother 58:4745–4754CrossRefPubMedPubMedCentralGoogle Scholar
- Sandur SK, Ichikawa H, Sethi G, Ahn KS, Aggarwal BB (2006) Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone) suppresses NF-kappaB activation and NF-kappaB-regulated gene products through modulation of p65 and IkappaBalpha kinase activation, leading to potentiation of apoptosis induced by cytokine and chemotherapeutic agents. J Biol Chem 281:17023–17033CrossRefPubMedGoogle Scholar
- Suzuki KT, Tanaka Y, Kawamura R (1983) Properties of metallothionein induced by zinc, copper and cadmium in the frog, Xenopus laevis. Comparative biochemistry and physiology. C, Comp Pharmacol Toxicol 75:33–37Google Scholar
- WHO (2015) Guidelines for the treatment of malaria-3rd edition. World Health Organization, Geneva, Switzerland. ISBN: 978-92-4154912-7Google Scholar
- Zhang W, Cheng L, Hou Y, Si M, Zhao YP, Nie L (2015) Plumbagin protects against spinal cord injury-induced oxidative stress and inflammation in wistar rats through Nrf-2 upregulation. Drug Res 65:495–499Google Scholar