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Enoxaparin pretreatment effect on local and systemic inflammation biomarkers in the animal burn model

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Abstract

Low-molecular weight heparins (LMWH) are anticoagulants that have shown anti-inflammatory activity in several experimental models. Hot water burn inflammatory model accurately simulates human clinical situations allowing its use for nociception test and evaluation of anti-inflammatory drugs. The present study aims to evaluate the enoxaparin pretreatment on local and systemic inflammation biomarkers in the animal burn model. Inflammation was induced by submersing the rat left hind paw in water at 60o C for 60 s. C-reactive protein (CRP) and thrombin–antithrombin complex (TAT) were estimated by immunosorbent assay, fibrinogen (Fg) by the gravimetric method and paw oedema by orthogonal digital photography. Highest values of paw oedema, CRP and TAT were observed at 4 h post-burn while Fg peak occurs at 12 h post-burn; enoxaparin pretreatment decreased oedema (− 32.1%), and concentration of TAT (− 66.7%), PCR (− 37.9%) and Fg (− 8%). This study shows that enoxaparin has local and systemic anti-inflammatory effects and should be considered as a potential adjuvant drug for the treatment of burns.

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Acknowledgements

We are grateful to the Central Biotherius of Faculty of Health Sciences of Carabobo University for the contribution of experimental animals.

Funding

This study was partially funded by the Scientific and Humanistic Development Council and by the Research Office of the Health Sciences Faculty, Carabobo University, Venezuela.

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Authors and Affiliations

  1. Faculty of Health Sciences, Research Unit of Lipids and Lipoproteins (INLIP), Carabobo University, Maracay, Venezuela

    Hember Vicci & Maria Navarro

  2. Faculty of Health Sciences, Center for Biophysics and Neuroscience (CBN), Carabobo University, Valencia, Venezuela

    Hember Vicci, Antonio Eblen-Zajjur & Maria Navarro

  3. Institute for Medical and Biological Engineering, Schools of Engineering, Biological Sciences and Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile

    Antonio Eblen-Zajjur

  4. Laboratory Hemostasis and Vascular Genetics, Center for Biophysic and Biochemistry (CBB), Venezuelan Institute for Scientific Research (IVIC), Caracas, Venezuela

    Mercedes López & Gustavo Crespo

  5. Final Av, Leonardo Ruiz Pineda, Facultad de Ciencias de la Salud, Universidad de Carabobo, La Morita, Maracay Estado Aragua, Venezuela

    Maria Navarro

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  1. Hember Vicci
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  2. Antonio Eblen-Zajjur
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Correspondence to Maria Navarro.

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Vicci, H., Eblen-Zajjur, A., López, M. et al. Enoxaparin pretreatment effect on local and systemic inflammation biomarkers in the animal burn model. Inflammopharmacol 27, 521–529 (2019). https://doi.org/10.1007/s10787-018-0444-6

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