Persea americana Mill. crude extract exhibits antinociceptive effect on UVB radiation-induced skin injury in mice

Deuschle, Viviane C. K. N.; Brusco, Indiara; Piana, Mariana; Faccin, Henrique; de Carvalho, Leandro Machado; Oliveira, Sara Marchesan; Viana, Carine

doi:10.1007/s10787-018-0441-9

Original Article
Published: 11 January 2018

Persea americana Mill. crude extract exhibits antinociceptive effect on UVB radiation-induced skin injury in mice

Viviane C. K. N. Deuschle¹,
Indiara Brusco²,
Mariana Piana¹,
Henrique Faccin³,
Leandro Machado de Carvalho^1,3,
Sara Marchesan Oliveira ORCID: orcid.org/0000-0003-2960-5284^2,4 &
Carine Viana¹

Inflammopharmacology volume 27, pages 323–338 (2019)Cite this article

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Abstract

Persea americana, popularly known as avocado, has been empirically used as analgesic and anti-inflammatory including in the skin disorder treatment. Species of the genus Persea also show a photoprotective effect against UVB radiation. We investigated the antinociceptive and anti-inflammatory effects from a topical formulation containing the P. americana leaf extract in a UVB irradiation-induced burn model in mice and performed a gel-formulation stability study. The antinociceptive and anti-inflammatory effect was evaluated through mechanical allodynia, paw oedema, and inflammatory cell infiltration. Phenolic compounds were quantified by UHPLC–MS/MS. The gel-formulation stability study was performed analyzing organoleptic characteristics, pH, and viscosity. P. americana (3%) gel was able to prevent the UVB irradiation-induced mechanical allodynia on the 2nd and 3rd day after irradiation with maximum inhibition of 60 ± 12% at 2nd day. Such effect may be attributed, at least in part, due the presence of (+)-catechin (302.2 ± 4.9 μg/g) followed by chlorogenic acid (130 ± 5.1 μg/g) and rutin (102.4 ± 0.9 μg/g) found in the extract. The gel was not able to prevent the inflammatory parameters such as edema and leukocyte infiltration induced by UVB irradiation. No changes important were detected in the stability study, mainly in low temperature. Our results suggest that P. americana gel-formulation, which presented stability, ensuring its quality and the therapeutic effect, could be an interesting strategy for the treatment of the pain associated with sunburn; this effect could be attributed to its biological constituents, especially catechin, chlorogenic acid, and rutin.

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Abbreviations

UVB:: Ultraviolet-B
UHPLC–MS/MS:: Ultra high-pressure liquid chromatography associated with mass spectrometry
EDTA:: Ethylenediamine tetraacetic acid
TMB:: 3,3′,5,5′-tetramethylbenzidine
NAGase:: N-acetyl-β-d-glucosaminidase
HTAB:: Hexadecyltrimethylammonium bromide
MRM:: Multiple reaction monitoring
ANVISA:: Brazilian regulatory agency (Agência Nacional de Vigilância Sanitária)
CEUA:: Ethics Committee for Animal Research
PWT:: Paw withdrawal threshold
MPO:: Myeloperoxidase
SEM:: Standard error of the mean

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Acknowledgements

This study was supported by the Conselho Nacional de Desenvolvimento Científico (CNPq) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS/CNPq). We also acknowledge fellowships from CNPq and Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES) (Brazil).

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Affiliations

Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil
Viviane C. K. N. Deuschle, Mariana Piana, Leandro Machado de Carvalho & Carine Viana
Graduate Program in Biological Sciences: Biochemistry Toxicology, Federal University of Santa Maria, Santa Maria, RS, Brazil
Indiara Brusco & Sara Marchesan Oliveira
Graduate Program in Chemistry, Federal University of Santa Maria, Santa Maria, RS, Brazil
Henrique Faccin & Leandro Machado de Carvalho
Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Av. Roraima 1000, Camobi, Santa Maria, RS, 97105-900, Brazil
Sara Marchesan Oliveira

Authors

Viviane C. K. N. Deuschle
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Indiara Brusco
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Mariana Piana
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Henrique Faccin
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Leandro Machado de Carvalho
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Sara Marchesan Oliveira
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Carine Viana
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Contributions

Participated in research design: VD, IB, CV, SMO conducted experiments: VD, IB, CV, SMO plant material and extractions, gel preparation, accelerated stability study, data analysis, writing and discussion of these: MP, VD UHPLC–MS/MS Chromatograph, data analysis, writing and discussion of these: HF, LMC performed data analysis: VD, IB, CV, SMO wrote or contributed to the writing of the manuscript: VD, IB, CV, SMO all the authors reviewed the manuscript.

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Correspondence to Sara Marchesan Oliveira.

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The authors declare that there are no conflicts of interest.

Ethical approval

All procedures performed were in accordance with international and national and were approved by Ethics Committee for Animal Research of the Federal University of Santa Maria (process number 1974200416/2016).

Additional information

Studied compounds: Silver Sulfadiazine: PubChem CID: 441244.

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Deuschle, V.C.K.N., Brusco, I., Piana, M. et al. Persea americana Mill. crude extract exhibits antinociceptive effect on UVB radiation-induced skin injury in mice. Inflammopharmacol 27, 323–338 (2019). https://doi.org/10.1007/s10787-018-0441-9

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Received: 05 December 2017
Accepted: 03 January 2018
Published: 11 January 2018
Issue Date: 01 April 2019
DOI: https://doi.org/10.1007/s10787-018-0441-9

Keywords

Avocado
Allodynia
Inflammation
Sunburn
Pain