Anti-inflammatory and antinociceptive properties of the hydroalcoholic fractions from the leaves of Annona crassiflora Mart. in mice

  • Cristina da Costa Oliveira
  • Natália Alves de Matos
  • Clarice de Carvalho Veloso
  • Gisele Avelar Lage
  • Lúcia Pinheiro Santos Pimenta
  • Igor Dimitri Gama Duarte
  • Thiago Roberto Lima Romero
  • André Klein
  • Andrea de Castro Perez
Original Article



Annona crassiflora Mart., popularly known as “Araticum”, is a native tree of the Brazilian Cerrado used in folk medicine for treatment of pain and inflammatory diseases. We proposed to analyze analgesic and anti-inflammatory properties of the filtrate (F1) and the precipitate (F2) of the hydroalcoholic fraction from the leaves of Annona crassiflora Mart. in mice.

Materials and methods

Swiss mice were submitted to formalin-induced nociception test and tail-flick reflex test, to assess antinociceptive properties, and to the rota-rod test, for motor performance analyses. To evaluate anti-inflammatory properties, F1 and F2 were orally administered 1 h prior to the intrathoracic injection of carrageenan, zymosan, LPS, CXCL8, or vehicle in Balb/c mice and neutrophil infiltration was evaluated 4 h after injection.


F1 and F2 reduced the licking time in the second phase of formalin-induced nociception test, but only F2 showed a dose-dependent response. Neither F1 nor F2 reduced the latency time in the tail-flick reflex test. In addition, motor performance alteration was not observed in F1- or F2-treated mice. F2 treatment significantly inhibited the neutrophilia induced by carrageenan, LPS, or CXCL8, but not zymosan.


The experimental data demonstrated that hydroalcoholic fractions of Annona crassiflora Mart. leaves have remarkable anti-inflammatory and antinociceptive activities.


Medicinal plants Annona crassiflora Mart. Peripheral antinociception Inflammatory pain CXCL8 



This work was supported by grants from Conselho Nacional de Pesquisa (CNPq/Brazil) and Fundação de Pesquisa do Estado de Minas Gerais (FAPEMIG/Brazil).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Cristina da Costa Oliveira
    • 1
  • Natália Alves de Matos
    • 1
  • Clarice de Carvalho Veloso
    • 1
  • Gisele Avelar Lage
    • 2
  • Lúcia Pinheiro Santos Pimenta
    • 2
  • Igor Dimitri Gama Duarte
    • 1
  • Thiago Roberto Lima Romero
    • 1
  • André Klein
    • 1
  • Andrea de Castro Perez
    • 1
  1. 1.Laboratory of Pain and Analgesia, Department of Pharmacology, Institute of Biological SciencesFederal University of Minas Gerais (UFMG)Belo HorizonteBrazil
  2. 2.Department of Chemistry, Institute of Exact SciencesUFMGBelo HorizonteBrazil

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