, Volume 26, Issue 3, pp 851–859 | Cite as

Foeniculum vulgare essential oil ameliorates acetic acid-induced colitis in rats through the inhibition of NF-kB pathway

  • Seyed Mahdi Rezayat
  • Ahmad-Reza Dehpour
  • Saeed Mohammadi Motamed
  • Maryam Yazdanparast
  • Mohsen Chamanara
  • Mousa Sahebgharani
  • Amir Rashidian
Original Article



The aim of the present study is to investigate the protective effects of Foeniculum vulgare essential oil on intestinal inflammation through the inhibition of NF-kB pathway in acetic acid-induced rat colitis.


Acute colitis was induced by intra-rectal administration of 2 mL of diluted acetic acid (4%) solution. Two hours after the induction of colitis, 0.2% tween 80 in normal saline, dexamethasone (2 mg/kg) and F. vulgare essential oil (100, 200, 400 mg/kg) were administered to the animals by oral gavage and continued for 5 consecutive days. Assessment of macroscopic and microscopic lesions was done. MPO activity was evaluated by biochemical method. Furthermore, TNF-α activity was detected by immunohistochemistry (IHC) and the expression level of p-NF-kB p65 protein was measured by western blot analysis.


Dexamethasone and F. vulgare essential oil (200, 400 mg/kg) reduced the macroscopic and microscopic lesions compared to the acetic acid group (p < 0.01, p < 0.001). In addition, these agents decreased the activity of MPO (p < 0.01, p < 0.001) and the expression of TNF-α positive cells (p < 0.05, p < 0.01, p < 0.001) in the colon tissue compared to acetic acid group. Furthermore, they inhibited acetic acid-induced expression of p-NF-kB p65 protein (p < 0.05, p < 0.001).


It is proposed that the anti-inflammatory activity of F. vulgare essential oil on acetic acid-induced colitis in rats may involve the inhibition of NF-kB pathway.


F. vulgare essential oil Ulcerative colitis Acetic acid NF-kB 


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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Seyed Mahdi Rezayat
    • 1
    • 2
  • Ahmad-Reza Dehpour
    • 1
    • 3
  • Saeed Mohammadi Motamed
    • 4
  • Maryam Yazdanparast
    • 3
  • Mohsen Chamanara
    • 1
  • Mousa Sahebgharani
    • 1
  • Amir Rashidian
    • 1
  1. 1.Department of Pharmacology, School of MedicineTehran University of Medical SciencesTehranIran
  2. 2.Department of Toxicology and Pharmacology, School of Pharmacy, Pharmaceutical Sciences BranchIslamic Azad University (IAUPS)TehranIran
  3. 3.Experimental Medicine Research CenterTehran University of Medical SciencesTehranIran
  4. 4.Department of Pharmacognosy, School of Pharmacy, Pharmaceutical Sciences BranchIslamic Azad University (IAUPS)TehranIran

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