, Volume 26, Issue 1, pp 217–226 | Cite as

Anti-inflammatory effect of a new piperazine derivative: (4-methylpiperazin-1-yl)(1-phenyl-1H-pyrazol-4-yl)methanone

  • Daniel C. Batista
  • Daiany P. B. Silva
  • Iziara F. Florentino
  • Carina S. Cardoso
  • Merita P. Gonçalves
  • Marize C. Valadares
  • Luciano M. Lião
  • Germán Sanz
  • Boniek G. Vaz
  • Elson A. Costa
  • Ricardo MenegattiEmail author
Original Article



This study investigates the anti-nociceptive and anti-inflammatory effects of new piperazine compound (LQFM182) as well as the toxicity acute in vitro.

Main methods

To evaluate the anti-nociceptive activity, the acetic acid-induced abdominal writhing test, tail flick test and formalin-induced pain test were used. The anti-inflammatory activity was evaluated using the models of paw oedema and pleurisy induced by carrageenan and some inflammatory parameters were evaluated, including cell migration, myeloperoxidase enzyme activity and the levels of TNF-α and IL-1β cytokines in pleural exudate. The acute oral systemic toxicity of LQFM182 in mice was evaluated through the neutral red uptake (nru) assay.

Key findings

LQFM182 (50, 100 or 200 mg/kg, p.o.) decreased the number of writhings induced by acetic acid in a dose-dependent manner, and an intermediate dose (100 mg/kg, p.o.) reduced the paw licking time of animals in the second phase of the formalin test. Furthermore, LQFM182 (100 mg/kg, p.o.) reduced oedema formation at all hours of the paw oedema induced by carrageenan test and in pleurisy test reduced cell migration from the reduction of polymorphonuclear cells, myeloperoxidase enzyme activity and the levels of pro-inflammatory cytokines IL-1β and TNF-α. Therefore, it was classified in GHS category 300 < LD50 < 2000 mg/kg.


Reduction of the TNF-α and IL-1β levels.


Anti-inflammatory N-arylheterocycles IL-1β TNF-α 



The authors are grateful to FUNAPE/UFG, PROCAD/CAPES and CNPq for financial support.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

10787_2017_390_MOESM1_ESM.doc (780 kb)
Supplementary material 1 (DOC 780 kb)


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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Daniel C. Batista
    • 1
  • Daiany P. B. Silva
    • 2
  • Iziara F. Florentino
    • 2
  • Carina S. Cardoso
    • 2
  • Merita P. Gonçalves
    • 2
  • Marize C. Valadares
    • 3
  • Luciano M. Lião
    • 4
  • Germán Sanz
    • 5
  • Boniek G. Vaz
    • 5
  • Elson A. Costa
    • 2
  • Ricardo Menegatti
    • 1
    Email author
  1. 1.Faculty of Pharmacy, Laboratory of Medicinal Pharmaceutical ChemistryFederal University of GoiásGoiâniaBrazil
  2. 2.Department of Pharmacology, ICBFederal University of GoiásGoiâniaBrazil
  3. 3.Laboratory of Pharmacology and Cell Toxicology, Faculty of PharmacyFederal University of GoiásGoiâniaBrazil
  4. 4.Chemistry InstituteFederal University of GoiasGoiâniaBrazil
  5. 5.Chemistry Institute, Laboratory of Chromatography and Mass Spectrometry-LaCEMFederal University of GoiásGoiâniaBrazil

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