, Volume 22, Issue 2, pp 105–114 | Cite as

Analgesic and antiinflammatory activities of the ethyl acetate fraction of Bidens pilosa (Asteraceae)

  • Aurélien Fotso Fotso
  • Frida LongoEmail author
  • Paul Désiré Dzeufiet DjomeniEmail author
  • Siméon Fogue Kouam
  • Michael Spiteller
  • Alain Bertrand Dongmo
  • J. P. Savineau
Research Article


Bidens pilosa is an Asteraceae widely used in traditional medicine for the treatment of various ailments including pain and inflammation. The present work was undertaken to assess the analgesic and antiinflammatory properties of the ethyl acetate fraction of methylene chloride/methanol (1:1) extract of leaves of Bidens pilosa at the gradual doses of 50, 100 and 200 mg/kg in mice and rats, respectively. The analgesic properties of Bidens pilosa were investigated using the acetic acid writhing, hot plate, capsaicin and formalin-induced pain models. This was followed by a study of the antiinflammatory properties using carrageenan, dextran, histamine and serotonin to induce acute inflammation in rat hind paw. The extract provided a significant (p < 0.01) reduction in pain induced by all four models of nociception. It also presented significant (p < 0.05) antiinflammatory activity in all four models of acute inflammation. These results show that the ethyl acetate fraction of methylene chloride/methanol (1:1) of Bidens pilosa has both analgesic and antiinflammatory properties. The qualitative analysis of the fraction by the high-performance liquid chromatography (HPLC) fingerprint revealed the presence of two flavonoids, namely quercetin and iso-okanin, known to have antiinflammatory and antinociceptive properties, which could be responsible for the analgesic and antiinflammatory effects observed.


Bidens pilosa Analgesic Antiinflammatory Flavonoids 


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Copyright information

© Springer Basel 2013

Authors and Affiliations

  • Aurélien Fotso Fotso
    • 1
  • Frida Longo
    • 1
    Email author
  • Paul Désiré Dzeufiet Djomeni
    • 2
    Email author
  • Siméon Fogue Kouam
    • 3
  • Michael Spiteller
    • 4
  • Alain Bertrand Dongmo
    • 5
  • J. P. Savineau
    • 6
  1. 1.Laboratory of Animal Physiology, Department of Biological Science, Higher Teachers’ Training CollegeUniversity of Yaoundé IYaoundéCameroon
  2. 2.Laboratory of Animal Physiology, Department of Animal Biology and Physiology, Faculty of ScienceUniversity of Yaoundé IYaoundéCameroon
  3. 3.Laboratory of Organic Chemistry, Department of Chemistry, Higher Teachers’ Training CollegeUniversity of Yaoundé IYaoundéCameroon
  4. 4.Institute of Environmental Research (INFU) of the Faculty of ChemistryDortmundGermany
  5. 5.Laboratory of Animal Organism Biology, Faculty of ScienceUniversity of DoualaDoualaCameroon
  6. 6.Cardiothoraxic Research Center of Bordeaux (INSERM U 1045)University of Victor Segalen Bordeaux 2Bordeaux cedexFrance

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