Abstract
Cleome gynandra L. (Capparidaceae) is one of the vegetables commonly known as ‘Hurhur’ and ‘Karaila’ in India, ‘Pe Hua Tsai’ in China and “Cat’s whiskers” in English. Present study was aimed to characterize previously isolated Cat’s whiskers flavonoid as 5-hydroxy-3, 7, 4′ -trimethoxyflavone (5HTMF) and to evaluate its effect on carrageenan-induced acute inflammation in rats and hydrogen peroxide induced DNA damage in mouse macrophages. The ex vivo effect of 5HTMF upon generation of free radicals in the mononuclear lymphocytes of patients with rheumatoid arthritis (RA) was also evaluated. 5HTMF not only reduce the swelling of hind paw in rats from 1 to 3 h of carrageenan injection but also decreased serum nitric oxide (NO) production. Toxic hydrogen peroxide induced oxidative DNA damage that was significantly decreased by 5HTMF. Though oxidative stress is a potential biomarker for determining disease activity in patients with RA, surprisingly 5HTMF inhibited the superoxide, hydroxyl and NO radicals in the isolated peripheral blood mononuclear lymphocytes of patients with RA. From the above study, it may be concluded 5HTMF attenuated acute inflammation by inhibiting NO and by protecting the oxidative DNA damage due to hydrogen peroxide scavenging property. It was also equally effective in scavenging the free radicals in lymphocytes of patients with RA. Collectively, our results indicate that 5HTMF as well as leafy vegetable of Cat’s whiskers may be a promising nontoxic food alternative in attenuating the oxidative stress, meriting further studies on other human inflammatory cells.
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Ansari NA, Sharma P, Salotra P (2007) Circulating nitric oxide and C-reactive protein levels in Indian kala azar patients: correlation with clinical outcome. Clin Immunol 122:343–348
Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, Cooper NS, Healey LA, Kaplan SR, Liang MH, Luthra HS (1988) The American rheumatism association 1987 revised criteria for the classification of rheumatoid arthritis. Arthr Rheum 31:315–324
Bala A, Kar B, Naskar S, Haldar PK, Mazumder UK (2009) Antioxidant activity of Cleome gynandra by different in vitro free radical scavenging models. J Interacadem 13:430–436
Bala A, Kar B, Haldar PK, Mazumder UK, Bera S (2010) Evaluation of anticancer activity of Cleome gynandra on Ehrlich’s Ascites carcinoma treated mice. J Ethnopharmacol 129:131–134
Bala A, Haldar PK, Kar B, Naskar S, Saha P, KunduSen S, Gupta M, Mazumder UK (2011) Antioxidant activity of the fractions of Cleome gynandra promotes antitumor activity in Ehrlich’s Ascites carcinoma. Asian J Chem 23:5055–5060
Bala A, Haldar PK, Kar B, Naskar S, Mazumder UK (2012a) Carbon tetrachloride: a hepatotoxin causes oxidative stress in murine peritoneal macrophage and peripheral blood lymphocyte cells. Immunopharmacol Immunotoxicol 34:135–142
Bala A, Kar B, Karmakar I, Kumar RBS, Haldar PK (2012b) Antioxidant activity of Cat’s whiskers flavonoid on some reactive oxygen and nitrogen species generating inflammatory cells is mediated by scavenging of free radicals. Chin J Nat Med 10:321–327
Bala A, Karmaka I, Haldar PK (2012c) Isolation and HPLC characterization of the flavonoid fractions from Cleome gynandra and comparative antioxidant activity. In: Govil JN, Geetanjali K (eds) Recent progress in medicinal plants- ethnomedicine and theraputic validation. Studium Press, USA, pp 225–240
Borgio JF, Thorat PK, Lonkar AD (2008) Toxicity of Gynandropsis pentaphylla DC extracts against microbials and its phytochemical profile. Ethnobot Leafl 12:320–336
Burton K (1955) A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Bio Chem J 62:315–323
Chaulya NC, Haldar PK, Mukherjee A (2010) In vitro free radical scavenging activity of methanol extract of rhizome of Cyperus tegetum roxb. (Cyperaceae). Inter J Curr Pharm Res 2:39–43
Chetia P, Bala A, Khandelwal B, Haldar PK (2012) Comparative in vitro free radical scavenging property of β carotene and naringenin with respect to vitamin C and N-acetyl cysteine. Pharmacologia 3:724–728
Daniela S, Zhi-Qiang W, Pamela SW, Bourdon DM, Marino MH, Manning PT, Currie MG (1996) Nitric oxide: a key mediator in the early and late phase of carrageenan induced rat paw inflammation. Br J Pharmacol 118:829–838
Das A, Bag S, Dua TK, Sinha MK, Gangopadhyay M, Dewanjee S (2010) Protective effect of Corchorus olitorius leaves on arsenic induced toxicity in experimental rats. Food Chem Toxicol 48:326–335
Dolai N, Karmakar I, Kumar RB, Kar B, Bala A, Haldar PK (2012) Evaluation of antitumor activity and in vivo antioxidant status of Anthocephalus cadamba on Ehrlich’s Ascites carcinoma treated mice. J Ethnopharmacol 142:865–870
Duthie SJ, Ma A, Ross MA, Collins AR (1996) Antioxidant supplementation decreases oxidative damage in human lymphocytes. Cancer Res 56:1291–1295
Guo D, Xu X, Cao X, Guo Y, Ye Y, Chan CO, Mok DK, Yu Z, Chen S (2011) Anti-inflammatory activities and mechanism of action of petroleum ether fraction of Rosa multiflora Thunb Hips. J Ethnopharmacol 138:717–722
Gupta AS, Chakravarty MM (1957) Studies on the seed for composition of desert plants. The component fatty acids of Gynandropsis pentaphylla seed fat. Sci Cult 23:306–307
Heever VD, Veenter SL (2007) Nutritional and medicinal properties of Cleome gynandra. Acta Hortic 752:127–130
Hitchon CA, El-Gabalawy HS (2004) Oxidation in rheumatoid arthritis. Arthr Res Ther 6:265–278
Jain AC, Gupta SM (1985) Minor phenolics components of the seed of Gynandropsis gynandra. J Nat Prod 48:332–333
Jocelyne G, Kevin B, Robert G, Joseph M (2004) Carrageenan induced paw edema in rat elicits a predominant prostaglandin E2 (PgE2) response in the central nervous system associated with the induction of microsomal PgE2 synthase -1. J Biol Chem 279:24866–24872
Kalaivani T, Rajasekaran C, Suthindhiran K (2011) Free radical scavenging, cytotoxic and hemolytic activities from leaves of Acacia nilotica (L.) Wild. ex. Delile subsp. indica (Benth.) Brenan. Evidence Based Compl Altern Med 274741:1–8
Kundu S, Bala A, Ghosh P, Mitra A, Sarkar A, Bauri AK, Ghosh A, Chattopadhyay S, Chatterjee M (2011) Attenuation of oxidative stress by allylpyrocatechol in synovial cellular infiltrate of patients with rheumatoid arthritis. Free Radic Res 45:518–526
Kundu S, Ghosh P, Datta S, Ghosh A, Chattopadhyay S, Chatterjee M (2012) Oxidative stress as a potential biomarker for determining disease activity in patients with rheumatoid arthritis. Free Radic Res 46:1482–1489
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Magari K, Miyata S, Ohkubo Y, Mutoh S (2004) Inflammatory cytokine levels in paw tissues during development of rat collagen-induced arthritis: effect of FK506, an inhibitor of T cell activation. Inflamm Res 53:469–474
Mollace V, Muscoli C, Masini E, Cuzzocrea S, Salvemini D (2005) Modulation of prostaglandin biosynthesis by nitric oxide and nitric oxide donors. Pharmacol Rev 57:217–252
Mukhopadhyay D, Das NK, Roy S, Kundu S, Barbhuiya JN, Chatterjee M (2011) Miltefosine effectively modulates the cytokine milieu in Indian post kala azar dermal leishmaniasis. J Infect Dis 20:1–10
Sarkar D, Saha P, Gamre S, Bhattacharjee S, Hariharan C, Ganguly S, Sen R, Mandal G, Chattopadhyay S, Majumdar S, Chatterjee M (2008) Anti-inflammatory effect of allylpyrocatechol in LPS-induced macrophages is mediated by suppression of iNOS and COX-2 via the NF-κB pathway. Inter Immunopharmacol 8:1264–1271
Sarkar D, Kundu S, De S, Hariharan H, Saha P, Manna A, Chattopadhyay S, Chatterjee M (2013) The antioxidant activity of Allylpyrocatechol is mediated via decreased generation of free radicals along with escalation of antioxidant mechanisms. Phytother Res 27(3):324–329
Acknowledgments
Financial assistance was provided by University Grant Commission (Rajiv Gandhi National Fellowship to Asis Bala), New Delhi and Dr. Dipankar Bala, Department of Pediatric Medicine, Burdwan Medical College, West Bengal, India is highly acknowledged.
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Bala, A., Chetia, P., Dolai, N. et al. Cat’s whiskers flavonoid attenuated oxidative DNA damage and acute inflammation: its importance in lymphocytes of patients with rheumatoid arthritis. Inflammopharmacol 22, 55–61 (2014). https://doi.org/10.1007/s10787-013-0193-5
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DOI: https://doi.org/10.1007/s10787-013-0193-5