Modulation of lipopolysaccharide-induced pro-inflammatory mediators by an extract of Glycyrrhiza glabra and its phytoconstituents
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To evaluate the inhibitory property of de-glycyrrhizinated extract of Glycyrrhiza glabra root and its phytoconstituents (glabridin, isoliquiritigenin and glycyrrhizin) on LPS-induced production of pro-inflammatory mediators.
Materials and methods
Inhibitory effect of G. glabra extract and its phytoconstituents were studied on lipopolysaccharide (LPS)-induced nitric oxide (NO), interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) levels in J774A.1 murine macrophages.
G. glabra and isoliquiritigenin significantly inhibited LPS stimulated NO, IL-1 beta and IL-6 production. Glabridin showed significant inhibition of NO and IL-1 beta release, but failed to attenuate IL-6 levels at the tested concentrations. In addition, glycyrrhizin did not exhibit inhibitory response towards any of the LPS-induced pro-inflammatory mediators at the tested concentrations.
From the results we speculate that the inhibitory effect of G. glabra extract on LPS-induced pro-inflammatory mediators is influenced by glabridin and isoliquiritigenin and is not contributed by glycyrrhizin.
KeywordsGlycyrrhiza glabra Phytoconstituents (glabridin, isoliquiritigenin and glycyrrhizin) Inflammation Pro-inflammatory mediators (NO, IL-1 beta and IL-6)
- Chandrasekaran CV, Deepak HB, Thiyagarajan P, Kathiresan S, Sangli GK (2010b) Dual inhibitory effect of Glycyrrhiza glabra (GutGard™) on COX and LOX products. Phytomedicine. doi: 10.1016/j.phymed.2010.08.001
- Kaczor T (2009) Deglycyrrhizinated licorice (DGL) for gastrointestinal ulcers: how can something so good be so poorly proven? Nat Med J 1Google Scholar
- Kakegawa H, Matsumoto H, Satoh T (1992) Inhibitory effects of some natural products on the activation of hyaluronidase and their anti-allergic actions. Chem Pharmaceut Bull (Tokyo) 40:1439–1442Google Scholar
- Nasyrov KhM, Lazareva DN (1980) Study of the anti-inflammatory activity of glycyrrhizic acid derivatives. Farmakol Toksikol (Moscow) 43:399–404Google Scholar
- Park SH, Kang JS, Yoon YD, Lee K, Kim KJ, Lee KH, Lee CW, Moon EY, Han SB, Kim BH, Kim HM, Park SK (2009) Glabridin inhibits lipopolysaccharide-induced activation of a microglial cell line, BV-2, by blocking NF-kappa B and AP-1. Phytother Res 24:S29–S34Google Scholar
- United States Pharmacoepia (USP) NF (2009) vol 32Google Scholar