, Volume 19, Issue 4, pp 235–241 | Cite as

Modulation of lipopolysaccharide-induced pro-inflammatory mediators by an extract of Glycyrrhiza glabra and its phytoconstituents

  • P. Thiyagarajan
  • C. V. ChandrasekaranEmail author
  • H. B. Deepak
  • Amit Agarwal
Short Communication



To evaluate the inhibitory property of de-glycyrrhizinated extract of Glycyrrhiza glabra root and its phytoconstituents (glabridin, isoliquiritigenin and glycyrrhizin) on LPS-induced production of pro-inflammatory mediators.

Materials and methods

Inhibitory effect of G. glabra extract and its phytoconstituents were studied on lipopolysaccharide (LPS)-induced nitric oxide (NO), interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) levels in J774A.1 murine macrophages.


G. glabra and isoliquiritigenin significantly inhibited LPS stimulated NO, IL-1 beta and IL-6 production. Glabridin showed significant inhibition of NO and IL-1 beta release, but failed to attenuate IL-6 levels at the tested concentrations. In addition, glycyrrhizin did not exhibit inhibitory response towards any of the LPS-induced pro-inflammatory mediators at the tested concentrations.


From the results we speculate that the inhibitory effect of G. glabra extract on LPS-induced pro-inflammatory mediators is influenced by glabridin and isoliquiritigenin and is not contributed by glycyrrhizin.


Glycyrrhiza glabra Phytoconstituents (glabridin, isoliquiritigenin and glycyrrhizin) Inflammation Pro-inflammatory mediators (NO, IL-1 beta and IL-6) 


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Copyright information

© Springer Basel AG 2011

Authors and Affiliations

  • P. Thiyagarajan
    • 1
  • C. V. Chandrasekaran
    • 1
    Email author
  • H. B. Deepak
    • 1
  • Amit Agarwal
    • 1
  1. 1.Department of Cellular AssayR&D Centre, Natural Remedies Pvt. LtdBangaloreIndia

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