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Treatment of experimental periodontal disease by a selective inhibitor of cyclooxygenase-2 with scaling and root planing (SRP)

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Abstract

Lumiracoxib is a selective inhibitor of cyclooxygenase-2 (COX-2) approved for the relief of symptoms of chronic inflammatory conditions. The aim of this study was to evaluate the effects of this specific inhibitor of COX-2 as adjunctive treatment on induced periodontitis in rats. Periodontal disease was induced at the first mandibular molar of 60 rats. After 7 days, the ligature was removed and all animals were submitted to scaling and root planing (SRP) along with local irrigation with saline solution and were divided into 2 groups: SRP (n = 30)—received subcutaneous injections of 1 mg/kg of body weight/day of saline solution for 3 days and; SRP + L (n = 30)—received subcutaneous injections of 1 mg/kg of body weight/day of Lumiracoxib for 3 days. Ten animals in each group were killed at 7, 15, and 30 days. The histological description was performed and the histometric values were statistically analyzed. In Group SRP + L, the histometric analysis (0.58 ± 0.08, 0.64 ± 0.06, and 0.56 ± 0.10 mm2) showed less bone loss (p < 0.05) than Group SRP (1.52 ± 0.08, 1.55 ± 0.09, and 1.49 ± 0.24 mm2) at 7, 15, and 30 days, respectively. Within the limits of this study it can be concluded that subcutaneous application of specific inhibitor of COX-2 was a beneficial adjunctive treatment for periodontal diseases induced in rats.

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References

  • Azoubel MC, Menezes AM, Bezerra D et al (2007) Comparison of etoricoxib and indomethacin for the treatment of experimental periodontitis in rats. Braz J Med Biol Res 40:117–125

    CAS  PubMed  Google Scholar 

  • Azoubel MC, Sarmento VA, Cangussú V (2008) Adjunctive benefits of etoricoxib in non-surgical treatment of aggressive periodontitis—short-term evaluation. J Periodontol 79:1719–1725

    Article  CAS  PubMed  Google Scholar 

  • Bezerra MM, de Lima V, Alencar VB (2000) Selective cyclooxygenase-2 inhibition prevents alveolar bone loss in experimental periodontitis in rats. J Periodontol 71:1009–1014

    Article  CAS  PubMed  Google Scholar 

  • Bissada NF, Ng VW (1998) Clinical evaluation of systemic doxycycline and ibuprofen administration as an adjunctive treatment for adult periodontitis. J Periodontol 69:772–776

    PubMed  Google Scholar 

  • Cavagni J, Soletti AC, Gaio EG et al (2005) The effect of dexamethasone in the pathogenesis of ligature-induced periodontal disease in Wistar rats. Braz Oral Res 19:290–294

    Article  PubMed  Google Scholar 

  • Chandrasekharan NV, Dai H, Roos KL (2002) COX-3, a cyclooxygenase-1 variant inhibited by acetaminophen and other analgesic/antipyretic drugs: cloning, structure, and expression. Proc Natl Acad Sci USA 99:13926–13931

    Article  CAS  PubMed  Google Scholar 

  • Crotti T, Smith MD, Hirsch R et al (2003) Receptor activator NF kappaB ligand (RANKL) and osteoprotegerin (OPG) protein expression in periodontitis. J Periodontal Res 38:380–387

    CAS  PubMed  Google Scholar 

  • De Almeida JM, Theodoro LH, Bosco AF et al (2008) Treatment of experimental periodontal disease by photodynamic therapy in rats with diabetes. J Periodontol 79:2156–2165

    Article  PubMed  Google Scholar 

  • Engelhardt G (1996) Pharmacology of meloxicam, a new non-steroidal anti-inflammatory drug with an improved safety profile through preferential inhibition of COX-2. Br J Rheumatol 35:4–12

    CAS  PubMed  Google Scholar 

  • Fleischmann R, Sheldon E, Maldonado-Cocco J et al (2006) Lumiracoxib is effective in the treatment of osteoarthritis of the knee: a prospective randomized 13-week study versus placebo and celecoxib. Clin Rheumatol 25:42–53

    Article  PubMed  Google Scholar 

  • Furlaneto FA, Nagata MJ, Fucini SE et al (2007) Bone healing in critical-size defects treated with bioactive glass/calcium sulfate: a histologic and histometric study in rat calvaria. Clin Oral Implants Res 18:311–318

    Article  PubMed  Google Scholar 

  • Haffajee AD, Cugini MA, Dibart S et al (1997) The effect of SRP on the clinical and microbiological parameters of periodontal diseases. J Clin Periodontol 24:324–334

    Article  CAS  PubMed  Google Scholar 

  • Hilário MOE, Terreri MT, Len CA (2006) Nonsteroidal anti-inflammatories, COX 2 inhibitors, indications, adverse effects. J Pediatric 82:206–212

    Article  Google Scholar 

  • Hofbauer LC, Lacey DL, Dunstan CR et al (1999) Interleukin-1beta and tumor necrosis factor-alpha, but not interleukin-6, stimulate osteoprotegerin ligand gene expression in human osteoblastic cells. Bone 25:255–259

    Article  CAS  PubMed  Google Scholar 

  • Holanda Pinto SA, Pinto LM, Cunha GM et al (2008) Anti-inflammatory effect of alpha, beta-amyrin, a pentacyclic triterpene from Protium heptaphyllum in rat model of acute periodontitis. Inflammopharmacology 16:48–52

    Article  CAS  PubMed  Google Scholar 

  • Holzhausen M, Rossa C Jr, Marcantonio E Jr et al (2002) Effect of selective cyclooxygenase-2 inhibition on the development of ligature-induced periodontitis in rats. J Periodontol 73:1030–1036

    Article  CAS  PubMed  Google Scholar 

  • Li KL, Vogel R, Jeffcoat MK (1996) The effect of ketoprofen creams on periodontal disease in rhesus monkeys. J Periodontol Res 31:525–532

    Article  Google Scholar 

  • Liu D, Xu JK, Figliomeni L et al (2003) Expression of RANKL and OPG mRNA in periodontal disease: possible involvement in bone destruction. Int J Mol Med 11:17–21

    PubMed  Google Scholar 

  • Lohinai Z, Stachlewitz R, Szekely AD et al (2001) Evidence for the expression of cyclooxygenase-2 enzyme in periodontitis. Life Sci 70:279–290

    Article  CAS  PubMed  Google Scholar 

  • Mangold JB, Gu H, Rodriguez LC et al (2004) Pharmacokinetics and metabolism of lumiracoxib in healthy male subjects. Drug Metab Dispos 32:566–571

    Article  CAS  PubMed  Google Scholar 

  • Meisel P, Kocher T (2005) Photodynamic therapy for periodontal diseases: state of the art. J Photochem Photobiol B 79:159–170

    Article  CAS  PubMed  Google Scholar 

  • Mitchell JA, Larkin S, Williams TJ (1995) Cyclooxygenase 2: regulation and relevance in inflammation. Biochem Pharmacol 5:1535–1542

    Article  Google Scholar 

  • Miyauchi M, Hiraoka M, Oka H (2004) Immuno- localization of COX-1 and COX-2 in the rat molar periodontal tissue after topical application of lipopolysaccharide. Arch Oral Biol 49:739–746

    Article  CAS  PubMed  Google Scholar 

  • Morton RS, Dongari-Bagtzoglou AI (2001) Cyclooxygenase-2 is upregulated in inflamed gingival tissues. J Periodontol 72:461–469

    Article  CAS  PubMed  Google Scholar 

  • Mousques T, Listgarten M, Phillips R (1980) Effect of scaling and root planning on the composition of the human subgingival microbial flora. J Periodontal Res 15:144–151

    Article  CAS  PubMed  Google Scholar 

  • Nogueira-Filho GR, Rosa BT, César-Neto JB et al (2007) Low- and high-yield cigarette smoke inhalation potentiates bone loss during ligature-induced periodontitis. J Periodontol 78:730–735

    Article  Google Scholar 

  • Nyman S, Schroeder HE, Lindhe J (1979) Suppression of Inflammation and bone resorption by indomethacin during experimental periodontitis in dogs. J Periodontol 50:450–461

    Article  CAS  PubMed  Google Scholar 

  • Offenbacher S, Heasman PA, Collins JG (1993) Modulation of host PGE2 secretion as a determinant of periodontal disease expression. J Periodontol 64:432–444

    CAS  PubMed  Google Scholar 

  • Oliveira TM, Sakai VT, Machado MA (2008) COX-2 inhibition decreases VEGF expression and alveolar bone loss during the progression of experimental periodontitis in rats. J Periodontol 79:1062–1069

    Article  CAS  PubMed  Google Scholar 

  • Page RC (2002) The etiology and pathogenesis of periodontitis. Compend Contin Educ Dent 23:11–14

    PubMed  Google Scholar 

  • Paquette DW, Williams RC (2000) Modulation of host inflammatory mediators as a treatment strategy for periodontal diseases. Periodontology 24:239–252

    Article  CAS  Google Scholar 

  • Petersilka GJ, Ehmke B, Flemming TF (2002) Antimicrobial effects of mechanical debridement. Periodontol 2000 28:56–71

    Google Scholar 

  • Rordorf CM, Choi L, Marshall P et al (2005) Clinical pharmacology of lumiracoxib: a selective cyclo-oxygenase-2 inhibitor. Clin Pharmacokinet 44:247–1266

    Article  Google Scholar 

  • Sato Y, Arai N, Negishi A et al (1997) Expression of cyclooxygenase genes and involvement of endogenous prostaglandin during osteogenesis in the rat tibial bone marrow cavity. J Med Dent Sci 44:81–92

    CAS  PubMed  Google Scholar 

  • Taiyeb Ali TB, Waite IM (1993) The effect of systemic ibuprofen on gingival inflammation in humans. J Clin Periodontol 20:723–728

    Article  CAS  PubMed  Google Scholar 

  • Umeda M, Takeuchi Y, Noguchi K et al (2004) Effects of non-surgical periodontal therapy on the microbiota. Periodontol 2000 36:98–120

    Article  PubMed  Google Scholar 

  • Weaks-Dybvig M, Sanavi F, Zander H et al (1982) The effect of indomethacin on alveolar bone loss in experimental periodontitis. J Periodontol Res 17:90–100

    Article  CAS  Google Scholar 

  • Williams RC, Paquette DW (2000) Understanding the pathogenesis of periodontitis: a century of discovery. J Int Acad Periodontol 2:59–63

    CAS  PubMed  Google Scholar 

  • Williams R, Jeffcoat MK, Howell TH (1988) Ibuprofen: an inhibitor of treatment of alveolar bone resorption in beagles. J Periodontol Res 23:225–229

    Article  CAS  Google Scholar 

  • Ximenez-Fyvie LA, Haffajee AD, Socransky SS (2000) Microbial composition of supra- and subgingival plaque in subjects with adult periodontitis. J Clin Periodontol 27:722–732

    Article  CAS  PubMed  Google Scholar 

  • Yen CA, Damoulis PD, Stark PC et al (2008) The effect of a selective cyclooxygenase-2 inhibitor (celecoxib) on chronic periodontitis. J Periodontol 79:104–113

    Article  CAS  PubMed  Google Scholar 

  • Zhang X, Schqarz EM, Young DA et al (2002) Cyclooxygenase-2 regulates mesenchymal cell differentiation into the osteoblast lineage and is critically involved in bone repair. J Clin Invest 110:1211

    Article  CAS  Google Scholar 

  • Zubery Y, Dunstan CR, Story BM (1998) Bone resorption caused by three periodontal pathogens in vivo in mice is mediated in part by prostaglandin. Infect Immun 66:4158–4162

    CAS  PubMed  Google Scholar 

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Acknowledgments

We thank to the Foundation of Support the Research of the State of São Paulo (Fapesp) for the financial support for the accomplishment of this research (Fapesp process 06/06358-0). This work is attributed to the Department of Periodontology, Araçatuba Dental School, State University of São Paulo (UNESP) Araçatuba, São Paulo, Brazil. The authors would also like to thank Bruno Theodoro Luciano, from the Institute of International Relations from the University of Brasília, for the English review.

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Authors and Affiliations

  1. GEPLO, Group of Research and Study on Laser in Dentistry, Department of Surgery and Integrated Clinic, Division of Periodontics, Univ Estadual Paulista, Araçatuba, São Paulo, Brazil

    Valdir Gouveia Garcia, Rodrigo Yuji Takano, Leandro Araújo Fernandes & Juliano Milanezi de Almeida

  2. Department of Periodontics, University Center of the Educational Foundation of Barrie (UNIFEB), Dental School Barry, Barry, SP, Brazil

    Valdir Gouveia Garcia & Leticia Helena Theodoro

  3. Department of Pathology and Clinical Dentistry, Federal University of Piauí, Teresina, PI, Brazil

    Leandro Araújo Fernandes

  4. Univ Estadual Paulista, Rua Jose Bonifácio 1193, Centro., CEP: 16050-300, Araçatuba, SP, Brazil

    Leandro Araújo Fernandes

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  1. Valdir Gouveia Garcia
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  2. Rodrigo Yuji Takano
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  3. Leandro Araújo Fernandes
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  4. Juliano Milanezi de Almeida
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  5. Leticia Helena Theodoro
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Correspondence to Leandro Araújo Fernandes.

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Garcia, V.G., Takano, R.Y., Fernandes, L.A. et al. Treatment of experimental periodontal disease by a selective inhibitor of cyclooxygenase-2 with scaling and root planing (SRP). Inflammopharmacol 18, 293–301 (2010). https://doi.org/10.1007/s10787-010-0059-z

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  • DOI: https://doi.org/10.1007/s10787-010-0059-z

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