Abstract
Bacopa monniera (L.) Wettst is a renowned plant in the Ayurvedic system of medicine. The present study seeks to identify the anti-inflammatory activity of two fractions from the methanolic extract of Bacopa, viz. the triterpenoid and bacoside-enriched fractions. The ability of these two fractions to inhibit the production of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-6 was tested using lipopolysaccharide (LPS)-activated peripheral blood mononuclear cells and peritoneal exudate cells in vitro. We found that triterpenoid and bacoside-enriched fractions significantly inhibited LPS-activated TNF-α, IL-6 and nitrite production in mononuclear cells. Significant antioxidant activity was exhibited by the bacoside enriched fraction compared to the triterpenoid fraction. Carrageenan-induced hind paw oedema assay revealed that triterpenoid and bacoside-enriched fractions exerted anti-oedematogenic effect, while in the arthritis model only the triterpenoid fraction exerted an anti-arthritic potential. The present study provides an insight into the ability of Bacopa monniera to inhibit inflammation through modulation of pro-inflammatory mediator release.
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Arend W, Deyer J (1995) Inhibition of production and effects of interleukin-1 and tumor necrosis factor-α in rheumatoid arthritis. Arthritis Rheum 38:151–152
Baldwin AS (2001) Series introduction: the transcription factor NF-κB and human disease. J Clin Invest 107:3–6
Bezerra MM, Brain SD et al (2004) Reactive nitrogen species scavenging, rather than nitric oxide inhibition, protects from articular cartilage damage in rat zymosan-induced arthritis. Br J Pharmacol 141:172–182
Chiou W, Chen C et al (2000) Mechanism of suppression of iNOS expression in RAW264.7 cells by Andrographolide. Br J Pharmacol 129:1553–1560
Chowdhury AR, Mandal S et al (2002) Betulinic acid, a potent inhibitor of eukaryotic topoisomerase I: identification of the inhibitory step, the major functional group responsible and development of more potent derivatives. Med Sci Monit 8:254–265
Chung H, Kang M et al (2007) Inhibition of nitric oxide and tumour necrosis factor-α by Moutan cortex in activated mouse peritoneal macrophages. Biol Pharm Bull 30:912–916
Darlington LG, Stone TW (2001) Antioxidants and fatty acids in the amelioration of rheumatoid arthritis and related disorders. Br J Nutr 85:251–269
Dhanasekaran M, Tharakan B et al (2007) Neuroprotective mechanisms of ayurvedic antidementia botanical Bacopa monniera. Phytother Res 21:965–969
Feghali CA, Timothy M et al (1997) Cytokines in acute and chronic inflammation. Front Biosci 2:12–26
Ferreira SH, Lorenzetti BB et al (1993) Bradikinin initiates cytokine mediated inflammatory hyperalgesia. Br J Pharmacol 110:1227–1231
Gauldie J, Richards C et al (1987) Interferon β2/B-cell stimulatory factor type 2 shares identity with monocyte-derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells. Proc Natl Acad Sci USA 84:7251–7255
Gilliam MB, Sherman MP et al (1993) A spectrophotometric assay for nitrate using NADPH oxidation by Aspergillus nitrate reductase. Anal Biochem 212:359–365
Haddad JJ (2002) Antioxidant and prooxidant mechanisms in the regulation of redox-sensitive transcription factors. Cell Signal 14:879–897
Hirano T, Akira S et al (1990) Biological and clinical aspects of interleukin-6. Immunol Today 11:443–449
Hurst SM, Wilkinson TS (2001) IL-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation. Immunity 14:705–714
Janeway C, Travers P et al (1999) The immune system in health and disease. In: Immunobiology. Garland Publishers, New York, pp 313–316
Jawaheer D, Thomson W et al (1994) Homozygosity for the HLA-DR shared epitope contributes the highest risk for rheumatoid arthritis concordance in identical twins. Arthritis Rheum 37:681–6866
Kakkar P, Das B (1984) A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 21:130–134
Kaplanski G, Marin V et al (2003) IL-6: a regulator of the transition from neutrophil to monocytes recruitment during inflammation. Trends Immunol 24:25–29
Kim SY, Kim SH et al (2007) Effects of Prunella vulgaris on mast cell-mediated allergic reaction and inflammatory cytokine production. Exp Biol Med 232:921–926
Kopf M, Baumann H et al (1994) Impaired immune and acute-phase responses in interleukin-6 deficient mice. Nature 368:339–342
Kotanidou A, Xagorarai A et al (2002) Luteolin reduces lipopolysaccharide-induced lethal toxicity and expression of pro-inflammatory molecules in mice. Am J Respir Crit Care Med 165:818–823
Lipsky PE (2006) Interleukin-6 and rheumatic diseases. Arthritis Res Ther 8:4–8
Lowry OH, Rosebrough NJ et al (1951) Protein measurement with Folin’s phenol reagent. J Biol Chem 193:265–273
MacMicking J, Xie QW et al (1997) Nitric oxide and macrophage function. Ann Rev Immunol 15:323–350
Nakamura M, Ferreira SH (1987) A peripheral sympathetic component in inflammatory hyperalgesia. Eur J Pharmacol 135:145–153
Okhawa H, Oshishi N et al (1979) Assay of lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–357
Rohini G, Devi CS (2008) Bacopa monniera extract induces apoptosis in murine sarcoma cells (S-180). Phytother Res 22:1595–1598
Sairam K, Rao CV et al (2001) Prophylactic and curative effects of Bacopa monniera in gastric ulcer models. Phytomedicine 8:423–430
Singh A, Singh SK (2009) Reversible suppression of spermatogenesis and fertility without toxic effects. Contraception 79:71–79
Schafer A, Chovanova Z et al (2005) Inhibition of COX-1 and COX-2 activity by plasma of human volunteers after ingestion of French maritime pine bark extract (Pycnogenol). Biomed Pharmacother 60:5–9
Taibi DM, Bourguignon C (2003) The role of complementary and alternative therapies in managing rheumatoid arthritis. Fam Community Health 26:41–52
Tracey K, Cerami A (1990) Metabolic response to cachectin/TNF. Annals of the New York, Academy of Sciences. New York, pp 325–330
Valko MD, Leibfritz J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44–84
Vijayan V, Helen A (2007) Protective activity of Bacopa monniera Linn. on nicotine-induced toxicity in mice. Phytother Res 21:378–381
Viji V, Helen A (2008) Inhibition of lipoxygenases and cyclooxygenase-2 enzymes by extracts isolated from Bacopa monniera (L.) Wettst. J Ethnopharmacol 118:305–311
Viji V, Kavitha SK et al (2010) Bacopa monniera (L.) Wettst inhibits type II collagen induced arthritis in rats. Phytother Res (PMID: 20309843) (in press)
Yun Y, Han S et al (2003) Immunomodulatory activity of betulinic acid by producing pro-inflammatory cytokines and activation of macrophages. Arch Pharmacol Res 26:1087–1095
Zhou Y, Peng L et al (2009) Effect of triterpenoid fractions from Bacopa monniera on scopolamine induced memory impairment in mice. Planta Med 75:568–574
Acknowledgments
This work was supported by the Kerala State Council for Science, Technology and Environment, Sasthra Bhavan, Thiruvananthapuram, Kerala, India. Standardized Bacopa extract was obtained as a gift from Natural Products Pvt Ltd., Bangalore, India.
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The authors declare that there were no conflicts of interest.
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Viji, V., Helen, A. Inhibition of pro-inflammatory mediators: role of Bacopa monniera (L.) Wettst. Inflammopharmacol 19, 283–291 (2011). https://doi.org/10.1007/s10787-010-0046-4
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DOI: https://doi.org/10.1007/s10787-010-0046-4