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Analgesic, anti-inflammatory and anti-platelet activities of the methanolic extract of Acacia modesta leaves

Inflammopharmacology volume 18, pages 187–196 (2010)Cite this article

Abstract

The current study was aimed to evaluate Acacia modesta for analgesic, anti-inflammatory, and anti-platelet activities. The analgesic and anti-inflammatory effects were assessed in rodents using acetic acid and formalin-induced nociception, hot plate and carrageenan-induced rat paw oedema tests. The intraperitoneal (i.p.) administration of the methanolic extract (50 and 100 mg/kg) produced significant inhibition (P < 0.01) of the acetic acid-induced writhing in mice and suppressed formalin-induced licking response of animals in both phases of the test. In the hot plate assay the plant extract (100 mg/kg) increased pain threshold of mice. Naloxone (5 mg/kg i.p.) partially reversed the analgesic effect of the extract in formalin and hot plate tests. A. modesta (100 and 200 mg/kg i.p.) exhibited sedative effect in barbiturate-induced hypnosis test similar to that produced by diazepam (10 mg/kg i.p.). The plant extract (50–200 mg/kg i.p.) produced marked anti-inflammatory effect in carrageenan-induced rat paw oedema assay comparable to diclofenac and produced a dose-dependent (0.5–2.5 mg/mL) inhibitory effect against arachidonic acid induced platelet aggregation. These data suggest that A. modesta possesses peripheral analgesic and anti-inflammatory properties, with analgesic effects partially associated with the opioid system.

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Affiliations

  1. Section Pharmacology, Shifa College of Medicine, Islamabad, Pakistan

    Ishfaq A. Bukhari

  2. Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan

    Ishfaq A. Bukhari & Rafeeq A. Khan

  3. Department of Biological and Biomedical Sciences, Aga Khan University and Medical College, Karachi, Pakistan

    Ishfaq A. Bukhari, Anwar H. Gilani, Sagheer Ahmed & Sheikh Arshad Saeed

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  1. Ishfaq A. Bukhari
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  2. Rafeeq A. Khan
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  3. Anwar H. Gilani
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  4. Sagheer Ahmed
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  5. Sheikh Arshad Saeed
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Correspondence to Ishfaq A. Bukhari.

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Bukhari, I.A., Khan, R.A., Gilani, A.H. et al. Analgesic, anti-inflammatory and anti-platelet activities of the methanolic extract of Acacia modesta leaves. Inflammopharmacol 18, 187–196 (2010). https://doi.org/10.1007/s10787-010-0038-4

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Keywords

  • Acacia modesta
  • Analgesic
  • Anti-inflammatory
  • Anti-platelet
  • Writhing test
  • Formalin test
  • Carrageenan-induced paw oedema