, Volume 18, Issue 3, pp 137–145 | Cite as

Evaluation of antiulcer and antisecretory potential of Linum usitatissimum fixed oil and possible mechanism of action

  • Gaurav Kaithwas
  • Dipak K. MajumdarEmail author
Research Article


The aim of the study was to evaluate the antiulcer activity of Linum usitatissimum fixed oil against aspirin-, indomethacin-, ethanol-, reserpine-, serotonin- and stress-induced gastric ulceration in rats and histamine-induced gastric ulceration in guinea pigs. Attempts were also made to evaluate the in vitro anticholinergic and antihistaminic activity and in vivo antisecretary and antiulcer activity of oil following pylorus ligation in rats. L. usitatissimum fixed oil exhibited significant antiulcer activity against different ulcerogens in experimental animal models. The fixed oil significantly inhibited acetylcholine- and histamine-induced contraction of guinea pig and rat ileums, respectively, suggesting its anticholinergic and antihistaminic activity. The oil also exhibited significant inhibitory effect on gastric secretion/total acidity and aspirin-induced gastric ulceration in pylorus-ligated rats. The lipoxygenase inhibitory, histamine antagonistic and antisecretory (anticholinergic) effects of the oil could probably have contributed towards antiulcer activity. L. usitatissimum fixed oil may be considered to be a drug of natural origin which possesses significant antiulcer activity. The present observation is the first experimental data showing antiulcer activity of L. usitatissimum fixed oil.


Lipoxygenase-inhibitor Antihistaminic Anticholinergic Antisecretory Flaxseed oil Linseed oil 



The authors would like to thank Arbro Pharmaceuticals Limited (New Delhi, India) for supplying drug samples, mentioned in the text, as generous gift. Thanks are also due, to Ranbaxy Research Laboratories (Gurgaon, India) for analysis of Linseed oil.


  1. Brodie DA, Hanson HM (1960) A study of the factors involved in the production of gastric ulcers by the restraint technique. Gastroenterology 38:353–360PubMedGoogle Scholar
  2. Cho CH, Pfeiffer CJ (1981) Gastrointestinal ulceration in the guinea pig in response to dimaprit, histamine and H1- and H2-blocking agents. Dig Dis Sci 26:306–311CrossRefPubMedGoogle Scholar
  3. Cho CH, Ogle CW, Dai S (1976) Acute gastric ulcer formation in response to electrical vagal stimulation in rats. Eur J Pharmacol 35:215–219CrossRefPubMedGoogle Scholar
  4. Cho CH, Ogle CW, Dai S (1985) A study on the etiology of reserpine ulceration and the antiulcer action of solcoseryl in rat stomach. J Pharm Pharmacol 37:823–825PubMedGoogle Scholar
  5. Goel RK, Bhattacharya SK (1991) Gastroduodenal mucosal defense and mucosal protective agents. Indian J Exp Biol 29:701–714PubMedGoogle Scholar
  6. Guth PH, Paulsen G, Nagata H (1984) Histologic and microcirculatory changes in alcohol-induced gastric lesions in the rat: effect of prostaglandin cytoprotection. Gastroenterology 87:1083–1090PubMedGoogle Scholar
  7. Hase T, Moss BJ (1973) Microvascular changes of gastric mucosa in the development of stress ulcers in rats. Gastroenterology 65:224–234PubMedGoogle Scholar
  8. Kaithwas G, Majumdar DK (2010) Therapeutic effect of Linum usitatissimum (flaxseed/linseed) fixed oil on acute and chronic arthritic models in albino rats. Inflammopharmacology. doi: 10.1007/s10787-010-0033-9
  9. Kirsner JB (1957) Drug-induced peptic ulcer. Ann Intern Med 47:666–699PubMedGoogle Scholar
  10. Kulkarni SK (2005) Experiment on isolated preparation, chapter 3. Hand book of experimental pharmacology, 3rd edn. Vallabh Prakashan, New Delhi, p 93Google Scholar
  11. Main IH, Whittle BJ (1975) Investigation of the vasodilator and antisecretory role of prostaglandins in the rat gastric mucosa by use of non-steroidal anti-inflammatory drugs. Br J Pharmacol 53:217–224PubMedGoogle Scholar
  12. Nkeh B, Kamany A, Bopelet M, Ayafor JF, Mbafor JT (1996) Inhibition of histamine induced contraction of rat ileum by promethazine and the methanol stembark extract of Erythrina sigmoidea (Hua). Phytother Res 10:444–446CrossRefGoogle Scholar
  13. Okabe S, Takata K, Tekeuchi K, Naganuma T, Takagi K (1974) Effects of carbenoxolone sodium on acute and chronic gastric ulcer models in experimental research. Am J Dig Dis 21:618–625CrossRefGoogle Scholar
  14. Parmar NS, Desai JK (1993) A review of the current methodology for the evaluation of gastric and duodenal antiulcer agents. Indian J Pharmacol 25:120–135Google Scholar
  15. Parnham MJ, Brune K (1987) Therapeutic control of inflammatory diseases. Agents Actions 21:232–234CrossRefGoogle Scholar
  16. Rainsford KD (1987) The effect of 5-lipoxygenase inhibitors and the leukotriene antagonists on the development of gastric lesions induced by non-steroidal anti-inflammatory drugs in mice. Agents Actions 21:316–319CrossRefPubMedGoogle Scholar
  17. Robert A, Nezamis JE, Lancaster C, Hanchar AJ (1979) Cytoprotection by prostaglandins in rats. Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl, and thermal injury. Gastroenterology 77:433–443PubMedGoogle Scholar
  18. Sandor V, Cuparencu B (1977) Analysis of the mechanism of the protective activity of some sympathomimetic amines in experimental ulcers. Pharmacology 15:208–217CrossRefPubMedGoogle Scholar
  19. Shay M, Komarov SA, Fels D, Meranze D, Gruenstein H, Siplet H (1945) A simple method for the uniform production of gastric ulceration in rats. Gastroenterology 5:43–61Google Scholar
  20. Singh S, Majumdar DK (1997) Evaluation of anti-inflammatory activity of fatty acids of Ocimum sanctum fixed oil. Indian J Exp Biol 35:380–383PubMedGoogle Scholar
  21. Singh S, Majumdar DK (1999) Evaluation of the gastric antiulcer activity of fixed oil of Ocimum sanctum (Holy Basil). J Ethnopharmacol 65(1):13–19CrossRefPubMedGoogle Scholar
  22. Singh S, Nair V, Jain S, Gupta YK (2008) Evaluation of anti-inflammatory activity of plant lipids containing α-linolenic acid. Indian J Exp Biol 46:453–456PubMedGoogle Scholar
  23. The Wealth of India (1976) Industrial products. Part IX. Publication and Information Directorate, CSIR, p 110Google Scholar
  24. The Wealth of India (2006) A dictionary of Indian raw materials and industrial products. Vol-4(J-Q), 1st supplement series, National Institute of Science Communication and Information Resources, CSIR, New Delhi, pp 41–44Google Scholar
  25. West GB (1982) Testing for drugs inhibiting the formation of gastric ulcers. J Pharmacol Methods 8:33–37CrossRefPubMedGoogle Scholar

Copyright information

© Springer Basel AG 2010

Authors and Affiliations

  1. 1.Department of Pharmaceutical Sciences, Faculty of Health and Medical SciencesAllahabad Agricultural Institute-Deemed UniversityAllahabadIndia
  2. 2.PharmaceuticsDelhi Institute of Pharmaceutical Sciences and Research (Formerly College of Pharmacy), University of DelhiNew DelhiIndia

Personalised recommendations