Abstract
It has been shown in a previous study that brown coal-derived potassium humate is safe and effective in suppressing contact hypersensitivity in rats. In this study the efficacy of potassium humate on other types of inflammation was determined. Preparative TLC followed by mass spectroscopy was used in an attempt to fingerprint the product. The effects of potassium humate, at an oral dose of 60 mg/kg bodyweight, on a delayed type hypersensitivity reaction, a carrageenan-induced inflammation model and an allogeneic graft-versus-host reaction (GVHR) in rats were investigated. Paw oedema was used as a measure of inflammation. It was found that potassium humate had no effect on the delayed type hypersensitivity reaction but significantly inhibited the increase in paw volume of the carrageenan-induced oedema in rats which compared favourably with indomethacin treatment. Furthermore, potassium humate inhibited the GVHR induced in normal and cyclophosphamide-treated immune-incompetent rats. The identification of a naturally occurring compound that is safe and effective in reducing different types of inflammation merits further evaluation in clinical trials.
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References
Antin JH, Ferrara JL (1992) Cytokine dysregulation and acute graft-versus-host disease. Blood 80:2964–2968
Baatz H (1988) Moortherapie en der frauenheilkunde. In: Flaig W, Goecke C, Kauffels W (eds) Moortherapie: Grundlagen und anwendungen. Ueberreuter, Berlin, pp 161–168
Baigorri R, Fuentes M, Gonzlez-Gaitano G et al (2009) Complementary multianalytical approach to study the distinctive structural features of the main humic fractions in solution: gray humic acid, brown humic acid, and fulvic Acid. J Agric Food Chem 2009(57):3266–3272
Bani S, Kaul A, Khan B et al (2005) Immunosuppressive properties of an ethyl acetate fraction from Euphorbia royleana. J Ethnopharmacol 99:185–192
Bergh JJ, Cronje IJ, Dekker J et al (1997) Non-catalytic oxidation of water-slurried coal with oxygen: identification of fulvic acids and acute toxicity. Fuel 76:149–154
Botes ME, Dekker J, van Rensburg CEJ (2002) Phase I trial with oral oxihumate in HIV-infected patients. Drug Dev Res 57:34–39
Gau RJ, Yang HL, Chow SN et al (2000) Humic acid suppresses the LPS-induced expression of cell-surface adhesion proteins through the inhibition of NF-κB activation. Toxicol Appl Pharm 166:59–67
Golbs S, Fuchs V, Kühnert M et al (1982) Pränataltoxikologische Testung von Huminsäuren an Laboratoriumsratten. Arch Exp Vet Med 36:179–185
Gutting BW, Bouzahzah F, Kong LK et al (2003) Oxazolone and diclofenac-induced PLN assay reactions are attenuated in mice orally pretreated with the respective compound: potential role of the induction of regulatory mechanisms following enteric administration. Toxicol Appl Pharm 189:120–133
Hartenstein R (1981) Sludge decomposition and stabilization. Science 212:743–749
Huber JD, Hau VS, Borg L, Campos CR, Egleton RD, Davis TP (2002) Blood–brain barrier tight junctions are altered during a 72-h exposure to λ-carrageenan-induced inflammatory pain. Am J Physiol Heart Circ Physiol 283:1531–1537
Jooné GK, van Rensburg CEJ (2004) An in vitro investigation of the anti-inflammatory properties of potassium humate. Inflammation 28:169–174
Kleinschmidt J (1988) Moortherapie bei rheumatischen erkrankungen. Flaig W, Goecke C, Kauffels W (eds) Moortherapie: Grundlagen und anwendungen. Ueberreuter, Berlin, Germany, pp 216–224
Kovarik R (1988) Über die anwendung von präparaten aus torf, bzw. Huminstoffen bei gynäkologischen erkrankungen. Flaig W, Goecke C, Kauffels W (eds) Moortherapie: Grundlagen und anwendungen. Ueberreuter, Berlin, pp 177–197
Lent W (1988) Bericht über die moorforschung und anwendung in der DDR, Polen, Tschechoslowakei Und UdSSR. In: Flaig W, Goecke C, Kauffels W (eds) Moortherapie: Grundlagen und anwendungen. Ueberreuter, Berlin, pp 169–176
Manosroi A, Saraphanchotiwitthaya A, Manosroi J (2005) In vivo immunomodulating activity of wood extracts from Clausena excavata Burm. J Ethnopharmacol 102:5–9
Nantel F, Denis D, Gordon R et al (1999) Distribution and regulation of cyclooxygenase-2 in carrageenan-induced inflammation. Br J Pharmacol 128:853–859
Paciolla MD, Kolla S, Jansen SA (2002) The reduction of dissolved iron species by humic acid and subsequent production of reactive oxygen species. Adv Environ Res 7:169–178
Pascher A, Klupp J, Langrehr J et al (2005) Anti-TNF-alpha therapy for acute rejection in intestinal transplantation. Transplant Proc 3:635–1636
Petersson M, Wiberg U, Lundeberg T et al (2001) Oxytocin decreases carrageenan-induced inflammation in rats. Peptides 22:479–1484
Recio MC, Giner RM, Uriburu L et al (2000) In vivo activity of pseudoguaianolide sesquiterpene lactones in acute and chronic inflammation. Life Sci 66:2509–2518
Satoshi K, Tsurufuji S (1985) Analysis of the factor(s) involved in the pathogenesis of zymosan-induced inflammation in rats. Japanese J Pharmacol 38:177–184
Schepetkin I, Khlebnikov A, Kwon BS (2002) Medical drugs from humus matter: focus on mumie. Drug Develop Res 57:40–159
Sharma KK, Mediratta PK, Reeta KH et al (2004) Acute and delayed restraint stress-induced changes in nitric oxide producing neurons in limbic regions. Neuroscience 125:981–993
Skowron-Cendrzak A, Bubak M, Dembowska J (1978) Local graft versus host reaction in the xenogeneic system. Arch Immunol Ther Exp 26:1087–1090
Smit HF, Kroes BH, Van den Berg A et al (2000) Immunomodulatory and anti-inflammatory activity of Picrorhiza scrophulariiflora. J Ethnopharmacol 73:101–109
Van Rensburg CEJ, Naude P (2009) Potassium humate inhibits complement activation and the production of inflammatory cytokines in vitro. Inflammation 32:270–276
Van Rensburg CEJ, Snyman JR, Mokoele T et al (2007) Brown coal derived humate inhibits contact hypersensitivity: an efficacy, toxicity and teratogenicity study in rats. Inflammation 30:148–152
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This research was supported by Unique Health Trust and a grant from the South African National Research Foundation (NRF). The corresponding author, C. E. J. van Rensburg, acts as consultant for the company.
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Naudé, P.J.W., Cromarty, A.D. & van Rensburg, C.E.J. Potassium humate inhibits carrageenan-induced paw oedema and a graft-versus-host reaction in rats. Inflammopharmacol 18, 33–39 (2010). https://doi.org/10.1007/s10787-009-0026-8
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DOI: https://doi.org/10.1007/s10787-009-0026-8