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Comparison of Photoacoustic Signals in Photosynthetic and Nonphotosynthetic Leaf Tissues of Variegated Pelargonium zonale

  • S. Veljović-JovanovićEmail author
  • M. Vidović
  • F. Morina
  • Lj. Prokić
  • D. M. Todorović
ICPPP 18
Part of the following topical collections:
  1. ICPPP-18: Selected Papers of the 18th International Conference on Photoacoustic and Photothermal Phenomena

Abstract

Green-white variegated leaves of Pelargonium zonale were studied using the photoacoustic method. Our aim was to characterize photosynthetically active green tissue and nonphotosynthetically active white tissue by the photoacoustic amplitude signals. We observed lower stomatal conductance and higher leaf temperature in white tissue than in green tissue. Besides these thermal differences, significantly higher absorbance in green tissue was based on chlorophyll and carotenoids which were absent in white tissue. However, optical properties of epidermal layers of both tissues were equal. The photoacoustic amplitude of white tissue was over four times higher compared to green tissue, which was correlated with lower stomatal conductance. In addition, at frequencies >700 Hz, the significant differences between the photoacoustic signals of green and white tissue were obtained. We identified the photoacoustic signal deriving from photosynthetic oxygen evolution in green tissue, using high intensity of red light modulated at 10 Hz. Moreover, the photoacoustic amplitude of green tissue increased progressively with time which corresponded to the period of induction of photosynthetic oxygen evolution. For the first time, very high frequencies (1 kHz to 5 kHz) were applied on leaf material.

Keywords

Pelargonium zonale Photoacoustic method Photosynthesis Stomatal conductance Thermal imaging Variegated plants 

Notes

Acknowledgments

This work was supported by the Ministry of Education, Science and Technological Development of Republic of Serbia (Projects Nos. III43010 and OI171016).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • S. Veljović-Jovanović
    • 1
    Email author
  • M. Vidović
    • 1
  • F. Morina
    • 1
  • Lj. Prokić
    • 2
  • D. M. Todorović
    • 1
  1. 1.Department of Materials Science, Institute for Multidisciplinary ResearchUniversity of BelgradeBelgradeSerbia
  2. 2.Faculty of AgricultureUniversity of BelgradeBelgradeSerbia

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