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Anaerobic Fungi in Gorilla (Gorilla gorilla gorilla) Feces: an Adaptation to a High-Fiber Diet?

  • Doreen Schulz
  • Moneeb A. Qablan
  • Ilona Profousova-Psenkova
  • Peter Vallo
  • Terence Fuh
  • David Modry
  • Alexander K. Piel
  • Fiona Stewart
  • Klara J. Petrzelkova
  • Kateřina Fliegerová
Article

Abstract

Many studies have demonstrated the importance of symbiotic microbial communities for the host with beneficial effects for nutrition, development, and the immune system. The majority of these studies have focused on bacteria residing in the gastrointestinal tract, while the fungal community has often been neglected. Gut anaerobic fungi of the class Neocallimastigomycetes are a vital part of the intestinal microbiome in many herbivorous animals and their exceptional abilities to degrade indigestible plant material means that they contribute significantly to fermentative processes in the enteric tract. Gorillas rely on a highly fibrous diet and depend on fermentative microorganisms to meet their daily energetic demands. To assess whether Neocallimastigomycetes occur in gorillas we analyzed 12 fecal samples from wild Western lowland gorillas (Gorilla gorilla gorilla) from Dzanga–Sangha Protected Areas, Central African Republic, and subjected potential anaerobic fungi sequences to phylogenetic analysis. The clone library contained ITS1 fragments that we related to 45 different fungi clones. Of these, 12 gastrointestinal fungi in gorillas are related to anaerobic fungi and our phylogenetic analyses support their assignment to the class Neocallimastigomycetes. As anaerobic fungi play a pivotal role in plant fiber degradation in the herbivore gut, gorillas might benefit from harboring these particular fungi with regard to their nutritional status. Future studies should investigate whether Neocallimastigomycetes are also found in other nonhuman primates with high fiber intake, which would also benefit from having such highly efficient fermentative microbes.

Keywords

Diet Gorillas Gut microbiome Neocallimastigales 

Notes

Acknowledgments

We are grateful to the government of the Central African Republic as well as the Ministre de l’Education Nationale, de l’Alphabetisation, de l’Enseignement Superieur, et de la Recherche for granting permission to conduct our research within the Dzanga–Sangha Protected Areas, Central African Republic. We further thank the World Wildlife Fund and the Primate Habituation Project for administrative and logistical support on side. Last, we are very grateful to the associate editor and the two anonymous reviewers for their valuable comments. The project was supported by the Leakey Foundation (D. Schulz, K. J. Petrzelkova, K. Fliegerová), by the project CEITEC (Central European Institute of Technology, CZ.1·05/1·1·00/02·0068) from the European Regional Development Fund (D. Modry), by project CZ.02.1.01/0.0/0.0/15_003/0000460 OP RDE (K. Fliegerová), by institutional support of Institute of Vertebrate Biology, Czech Academy of Sciences (RVO: 68081766) (K. J. Petrzelkova) and cofinanced from the European Social Fund and the state budget of the Czech Republic (CZ.1·07/2·3·00/20·0300) (D. Schulz, I. Profousova-Psenkova, M. A. Qablan, D. Modry, K. J. Petrzelkova).

Supplementary material

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Table S1 (XLSX 16 kb)
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Table S2 (XLSX 13 kb)
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Table S3 (TXT 10 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Doreen Schulz
    • 1
    • 2
    • 3
  • Moneeb A. Qablan
    • 4
  • Ilona Profousova-Psenkova
    • 5
  • Peter Vallo
    • 2
    • 6
  • Terence Fuh
    • 7
  • David Modry
    • 3
    • 8
    • 9
  • Alexander K. Piel
    • 10
    • 11
  • Fiona Stewart
    • 10
    • 11
  • Klara J. Petrzelkova
    • 2
    • 9
    • 12
  • Kateřina Fliegerová
    • 13
  1. 1.Department of Botany and ZoologyMasaryk UniversityBrnoCzech Republic
  2. 2.Institute of Vertebrate BiologyCzech Academy of SciencesBrnoCzech Republic
  3. 3.Department of Pathology and ParasitologyUniversity of Veterinary and Pharmaceutical SciencesBrnoCzech Republic
  4. 4.Veterinary Medicine Department, College of Food and AgricultureUnited Arab Emirates UniversityAl AinUnited Arab Emirates
  5. 5.Zoological Garden Ústí nad LabemÚstí nad LabemCzech Republic
  6. 6.Institute of Evolutionary Ecology and Conservation GenomicsUniversity of UlmUlmGermany
  7. 7.Primate Habituation Project, World Wildlife Fund, Dzanga–Sangha Protected AreasBanguiCentral African Republic
  8. 8.CEITEC-VFUUniversity of Veterinary and Pharmaceutical SciencesBrnoCzech Republic
  9. 9.Institute of Parasitology, Biology CentreCzech of the Academy of SciencesČeské BudějoviceCzech Republic
  10. 10.School of Natural Sciences and PsychologyLiverpool John Moores UniversityLiverpoolUK
  11. 11.Ugalla Primate ProjectUvinzaTanzania
  12. 12.Liberec ZooLiberecCzech Republic
  13. 13.Institute of Animal Physiology and GeneticsCzech Academy of SciencesPragueCzech Republic

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