Recent evidence of pathogen transmission to humans from wild primates and a greater recognition of the risk of human pathogen transmission to free-ranging primates have raised awareness of the potential impact of zoonotic pathogen transmission on primate conservation and nonhuman primate and human health. Cryptosporidium and Giardia are zoonotic protozoan parasites transmitted via fecal–oral contamination or water that can cause gastritis or enteritis in human and nonhuman primates. From June 2002 to September 2003, we collected fecal samples noninvasively from two species of tamarins (Saguinus mystax and S. nigrifrons) and one species of titi monkeys (Callicebus cupreus) at the Estación Biológica Quebrada Blanco in the Peruvian Amazon to determine the distribution and prevalence of these potential pathogens. We screened 140 fecal samples representing known individuals of each species for Cryptosporidium and Giardia using the Merifluor immunoflourescence assay to determine the prevalence and intensity of infection with these organisms. With the exception of two samples we collected during the same week from a juvenile male Saguinus mystax, all samples were negative for Cryptosporidium. None of the fecal samples were positive for Giardia. The low prevalence of infection we observed limited our ability to examine the effects of demographic and environmental variables on patterns of infection; however, the exceptionally low prevalence of Cryptosporidium suggests that it is not a current health threat to these primate populations. Although the origin of infection with Cryptosporidium in the juvenile male Saguinus mystax cannot be determined, its presence alerts us to the potential for cross-species transmission and highlights the need for more detailed research to improve our understanding of the distribution and diversity of potentially pathogenic protozoa in Neotropical primate populations.
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Adam, R. D. (2001). Biology of Giardia lamblia. Clinical Microbiology Review, 14, 447–475.
Appelbee, A. J., Thompson, R. C. A., & Olson, M. E. (2005). Giardia and Cryptosporidium in mammalian wildlife – current status and future needs. Trends in Parasitology, 21, 370–376.
Calvignac-Spencer, S., Leendertz, S. A., Gillespie, T. R., & Leendertz, F. H. (2012). Wild great apes as sentinels and sources of infectious disease. Clinical Microbiology and Infection, 18(6), 521–527.
Gillespie, T. R. (2006). Non-invasive assessment of gastro-intestinal parasite infections in free-ranging primates. International Journal of Primatology, 27, 1129–1143.
Gillespie, T. R., & Chapman, C. A. (2006). Forest fragment attributes predict parasite infection dynamics in primate metapopulations. Conservation Biology, 20, 441–448.
Gillespie, T. R., & Chapman, C. A. (2008). Forest fragmentation, the decline of an endangered primate, and changes in host-parasite interactions relative to an unfragmented forest. American Journal of Primatology, 70, 222–230.
Gillespie, T. R., Morgan, D., Deutsch, J. C., Kuhlenschmidt, M. S., Salzer, J. S., Cameron, K., Reed, P., & Sanz, C. (2009). A legacy of low impact logging does not elevate prevalence of potentially pathogenic protozoa in free-ranging chimpanzees and lowland gorillas in the Republic of Congo. EcoHealth, 6, 557–564.
Heymann, E. W. (1995). Sleeping habits of tamarins, Saguinus mystax and Saguinus fuscicollis (Mammalia; Primates; Callitrichidae), in north-eastern Peru. Journal of Zoology, London, 237, 211–226.
Heymann, E. W., & Buchanan-Smith, H. M. (2000). The behavioural ecology of mixed species troops of callitrichine primates. Biological Reviews, 75, 169–190.
Johnston, A., Gillespie, T. R., Rwego, I. B., Tranby, T. L., Kent, A. D., & Goldberg, T. L. (2010). Molecular epidemiology of cross-species Giardia duodenalis transmission in western Uganda. PLoS Neglected Tropical Diseases, 4, e683.
Köndgen, S., Kühl, H., N’Goran, P. K., et al. (2008). Pandemic human viruses cause decline of endangered great apes. Current Biology, 18, 260–264.
Kowalewski, M., Salzer, J. S., Deutsch, J. C., Raño, M., Kuhlenschmidt, M. S., & Gillespie, T. R. (2011). Black and gold howler monkeys (Alouatta caraya) as sentinels of ecosystem health: Patterns of zoonotic protozoa infection relative to degree of human–primate contact. American Journal of Primatology, 73, 75–83.
Monis, P. T., & Thompson, R. C. (2003). Cryptosporidium and Giardia-zoonoses: Fact or fiction? Infection, Genetics and Evolution, 3, 233–244.
Muchiri, J. M., Ascolillo, L., Mugambi, M., Mutwiri, T., Ward, H. D., Naumova, E. N., et al. (2009). Seasonality of Cryptosporidium oocyst detection in surface waters of Meru, Kenya as determined by two isolation methods followed by PCR. Journal of Water Health, 7, 67–75.
Müller, B. (2007). Determinants of the diversity of intestinal parasite communities in sympatric New World primates. Hannover: Inaugural dissertation.
Nizeyi, J. B., Cranfield, M. R., & Graczyk, T. K. (2002a). Cattle near the Bwindi Impenetrable National Park, Uganda, as a reservoir of Cryptosporidium parvum and Giardia duodenalis for local community and free-ranging gorillas. Parasitology Research, 88, 380–385.
Nizeyi, J. B., Mwebe, R., Nanteza, A., Cranfield, M. R., Kalema, G. R., & Graczyk, T. K. (1999). Cryptosporidium sp. and Giardia sp. infections in mountain gorillas (Gorilla gorilla beringei) of the Bwindi Impenetrable National Park, Uganda. Journal of Parasitology, 85(6), 1084–1088.
Nizeyi, J. B., Sebunya, D., Dasilva, A. J., Cranfield, M. R., Pieniazek, N. J., & Graczyk, T. K. (2002b). Cryptosporidiosis in people sharing habitats with free-ranging mountain gorillas (Gorilla gorilla beringei), Uganda. American Journal of Tropical Medicine and Hygiene, 66, 442–444.
Palacios, G., Lowenstine, L. J., Cranfield, M. R., et al. (2011). Human metapneumovirus infection in wild mountain gorillas, Rwanda. Emerging Infectious Diseases, 17, 711–713.
Rasambainarivo, F. T., Gillespie, T. R., Wright, P. C., Arsenault, J., Villeneuve, A., & Lair, S. (2013). Survey of Giardia and Cryptosporidium in lemurs from the Ranomafana National Park, Madagascar. Journal of Wildlife Diseases, 49(3), 741–743.
Salyer, S. J., Gillespie, T. R., Rwego, I. B., & Chapman, C. A. (2012). Epidemiology and molecular ecology of Cryptosporidium spp. in people, primates, and livestock from western Uganda. PLoS Neglected Tropical Diseases, 6(4), e1597.
Salzer, J. S., Rwego, I. B., Kuhlenschmidt, M. S., & Gillespie, T. R. (2007). Giardia sp. and Cryptosporidium sp. infections in primates in fragmented and undisturbed forest in western Uganda. Journal of Parasitology, 93, 439–440.
Savioli, L., Smith, H., & Thompson, A. (2006). Giardia and Cryptosporidium join the ‘Neglected Diseases Initiative. Trends in Parasitology, 22, 203–208.
Schaumburg, F., Mugisha, L., Peck, B., Becker, K., Gillespie, T. R., Peters, G., & Leendertz, F. H. (2012). Drug-resistant human Staphylococcus aureus in sanctuary apes pose a threat to endangered wild ape populations. American Journal of Primatology, 74, 1071–1075.
Soini, P., & de Soini, M. (1982). Distribución geográfica y ecología poblacional de Saguinus mystax (Primates, Callitrichidae). Informe de Pacaya, 6, 1–56.
Thompson, R. C. A. (2004). Epidemiology and zoonotic potential of Giardia infections. In C. R. Sterling & R. D. Adam (Eds.), The pathogenic enteric protozoa: Giardia, Entamoeba, Cryptosporidium and Cyclospora. World Class Parasites, 8, 1–14.
Young, H., Griffin, R. H., Wood, C. L., & Nunn, C. L. (2013). Does habitat disturbance increase infectious disease risk for primates? Ecology Letters. doi:10.1111/ele.12094.
All work complied with legal requirements of the countries (Peru, Germany, USA) where the respective parts of the study were conducted and with the institutional ethical guidelines. Fieldwork in Peru and exportation of the material was conducted under permission from the Instituto Nacional de Recursos Naturales (INRENA) in Lima. We are grateful to Ney Shahuano Tello for field assistance, Johanna Salzer and Eunice Lee for technical assistance, and Joanna Setchell and three anonymous reviewers for helpful comments on earlier drafts of this manuscript. Funding for this project was provided by Emory University, the German Academic Exchange Service (DAAD), and the German Research Foundation (DFG).
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West, K.A., Heymann, E.W., Mueller, B. et al. Patterns of Infection with Cryptosporidium sp. and Giardia sp. in Three Species of Free-Ranging Primates in the Peruvian Amazon. Int J Primatol 34, 939–945 (2013). https://doi.org/10.1007/s10764-013-9710-z
- Callicebus cupreus
- Saguinus mystax
- Saguinus nigrifrons