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Siglec-E Ligand Downregulation on Hippocampus Neurons Induced Inflammation in Sevoflurane-Associated Perioperative Neurocognitive Disorders in Aged Mice

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Abstract

Activated microglia-induced inflammation in the hippocampus plays an important role in perioperative neurocognitive disorders. Previous studies have shown that sialic acid-binding immunoglobulin-like lectin 3 (hSiglec-3, ortholog of mouse Siglec-E) engagement in microglia and its glycan ligands on neurons contributes to inflammatory homeostasis through an endogenous negative regulation pathway. This study aimed to explore whether the glycan ligand alteration on neurons plays a role in sevoflurane-induced perioperative neurocognitive disorders. This study’s data has shown that a slight Siglec-E ligands’ expression decrease does not induce inflammation homeostasis disruption. We also demonstrated that the ligand level on neurons was decreased with age, and the reduced Siglec-E ligand expression on neurons caused via sevoflurane was induced by neuraminidase 1. Furthermore, this study has shown that the Siglec-E ligand expression decline caused by age and sevoflurane treatment could decrease the ligands’ level, thus leading to inflammatory homeostasis disruption. This research provided a novel mechanism for perioperative neurocognitive disorder susceptibility in the elderly.

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Data Availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

This study was supported by the National Natural Science Foundation of China (No. 81971018), the 345 Talent Project of Shengjing Hospital of China Medical University, and the Applied Basic Research Plan of Liaoning Province (Joint Plan, 2022JH2/101500065).

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Authors and Affiliations

  1. Department of Critical Care Medicine, Shengjing Hospital of China Medical University, Shenyang, Liaoning, 110004, China

    Xiaoli Zhang

  2. Department of Anesthesiology, Shengjing Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang, Liaoning, 110004, China

    Xueting Wang, Ziyang Xu, Fengwei Sun & Yue Tian

  3. Institute of Materia Medica and Department of Pharmaceutics, College of Pharmacy, Army Medical University, Chongqing, 400038, China

    Yi Jia

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  1. Xiaoli Zhang
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Contributions

Yue Tian and Yi Jia designed the research. Xiaoli Zhang and Xueting Wang performed the cell experiments and flow cytometry. Ziyang Xu contributed to the animal treatment and cognitive function analysis. Fengwei Sun performed immunofluorescence. Xiaoli Zhang and Yue Tian wrote the manuscript. Yue Tian and Yi Jia revised the manuscript.

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Correspondence to Yue Tian.

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Zhang, X., Wang, X., Xu, Z. et al. Siglec-E Ligand Downregulation on Hippocampus Neurons Induced Inflammation in Sevoflurane-Associated Perioperative Neurocognitive Disorders in Aged Mice. Inflammation 47, 30–44 (2024). https://doi.org/10.1007/s10753-023-01888-1

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