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Recombinant Irisin Protects Against Alveolar Bone Destruction During Orthodontic Tooth Movement

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Abstract

Patients with periodontitis have higher risk of alveolar bone loss when seek for orthodontic therapy. Inflammation and osteogenesis are key factors in alveolar bone destruction in periodontitis and orthodontic tooth movement (OTM). Here we evaluated the effects of irisin on alveolar bone destruction in rats with periodontitis and OTM. We isolated and cultured human periodontal ligament stem cells (PDLSCs). Irisin was administrated to PDLSCs. Cell proliferations, osteogenic differentiation, expression of RUNX2 and ALP, and the expression of OPG and RANKL were measured. We induced periodontitis and OTM in rats and treated rats with irisin. The alveolar bone loss, inflammatory cytokine levels, and expression of OPG and RANKL in gingival tissues were measured. Irisin promoted the cell proliferation and osteogenic differentiation of PDLSCs. Irisin elevated the expression of RUNX2, ALP, and OPG while decreased the expression of RANKL in PDLSCs. Irisin ameliorated the alveolar bone loss, suppressed cytokine levels, and increased OPG/RANKL expression ratio in rat with periodontitis and orthodontic tooth movement. Irisin prevented alveolar bone destruction during OTM in rats with periodontitis.

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DATA AVAILABILITY

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

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Authors and Affiliations

  1. Department of Stomatology Clinic, Cangzhou Central Hospital, No. 16 Xinhua West Road, Cangzhou, 061000, Hebei, China

    Shuainan Wu, Jianqi Wang, Lu Liu, Ying Pang, Zhaobao Li & Hong Mu

Authors
  1. Shuainan Wu
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  2. Jianqi Wang
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  3. Lu Liu
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  4. Ying Pang
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  5. Zhaobao Li
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  6. Hong Mu
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Contributions

Shuainan Wu: data curation, conceptualization, methodology, writing—original draft preparation, investigation, methodology, writing—reviewing and editing. Jianqi Wang: data curation, software, validation. Lu Liu: visualization, data curation. Ying Pang: data curation, conceptualization, writing—original draft preparation, supervision, writing—reviewing and editing. Zhaobao Li: conceptualization, visualization, data curation. Hong Mu: conceptualization, visualization, data curation.

Corresponding author

Correspondence to Shuainan Wu.

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Wu, S., Wang, J., Liu, L. et al. Recombinant Irisin Protects Against Alveolar Bone Destruction During Orthodontic Tooth Movement. Inflammation 46, 1106–1117 (2023). https://doi.org/10.1007/s10753-023-01797-3

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  • DOI: https://doi.org/10.1007/s10753-023-01797-3

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