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Anti-inflammatory and Antioxidant Effects of Rosuvastatin on Asthmatic, Hyperlipidemic, and Asthmatic-Hyperlipidemic Rat Models

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Abstract

Statins could be of potential therapeutic effect in asthma due to their pleiotropic effects on inflammation process. This study aimed to examine the possible interaction of serum lipids, and evaluate the effect of rosuvastatin treatment on asthma. Seven groups of rats, namely control (C), asthmatic (A), hyperlipidemic (H), asthmatic-hyperlipidemic (AH), rosuvastatin (40 mg/kg)-treated asthmatic (AR), rosuvastatin-treated hyperlipidemic (HR), and rosuvastatin-treated hyperlipidemic-asthmatic (AHR) groups, were studied. Total and differential WBC counts, serum oxidative stress markers, and bronchoalveolar lavage fluid (BALF) levels of IL-6 and IL-10 were evaluated. In the A and AH groups, total and differential WBC counts, and IL-6 and IL-10 levels were higher than in the C group (p<0.05 to p<0.001). An increase in nitrite and malondialdehyde concentrations and a decrease in total thiol content and superoxide dismutase and catalase activities were observed in the A, H, and AH groups compared to the C group (p<0.05 to p<0.001). Beyond lipid lowering, rosuvastatin treatment reduced total and differential WBC counts in the A and AH groups (p<0.05 to p<0.001), IL-6 level in the AH group (p<0.05), and IL-10 level in all treated groups (p<0.05). Rosuvastatin reduced oxidative stress by decreasing nitrite and malondialdehyde concentrations, and increasing total thiol content in all treated groups as well as superoxide dismutase and catalase activities in the H and AH groups (p<0.05 to p<0.01). Rosuvastatin reduced airway inflammation and oxidation through regulating NOS and reducing pro-inflammatory cytokine and inflammatory cells, which indicate a novel insight into the pleiotropic effects of rosuvastatin in treatment of asthma.

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Availability of Data and Materials

The data of this study are available from the corresponding author upon request.

Funding

This work was supported by a grant from Research Council of Mashhad University of Medical Sciences (Grant Number: 940997).

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Authors and Affiliations

  1. Department of Physiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

    Saeideh Saadat

  2. Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Saeideh Saadat & Mohammad Hossein Boskabady

  3. Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohammad Hossein Boskabady

Authors
  1. Saeideh Saadat
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  2. Mohammad Hossein Boskabady
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Contributions

SS carried out the experimental work, performed statistical analysis, prepared figures, and drafted the first version of the manuscript. MHB designed and supervised the study, helped in statistical analysis, and corrected the manuscript. Both authors read and approved the final manuscript.

Corresponding author

Correspondence to Mohammad Hossein Boskabady.

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The study was approved by the Ethics Committee of Mashhad University of Medical Sciences for Animal Experiments (Approval No. 940997).

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Not applicable.

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The authors declare no competing interests.

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Saadat, S., Boskabady, M.H. Anti-inflammatory and Antioxidant Effects of Rosuvastatin on Asthmatic, Hyperlipidemic, and Asthmatic-Hyperlipidemic Rat Models. Inflammation 44, 2279–2290 (2021). https://doi.org/10.1007/s10753-021-01499-8

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  • DOI: https://doi.org/10.1007/s10753-021-01499-8

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