Abstract
Periodontitis is a common chronic inflammation that often occurs in adults. Circular RNAs (circRNAs) play a vital role in inflammation-related diseases. However, the role and potential basis of hsa_circ_0085289 in periodontitis remain unknown. Periodontal ligament cells (PDLCs) were exposed to lipopolysaccharide (LPS) to mimic periodontitis. The levels of circ_0085289, let-7f-5p, and suppressor of cytokine signaling 6 (SOCS6) were determined using qRT-PCR and western blot. The release of inflammatory cytokines was measured via enzyme-linked immunosorbent assay (ELISA). Cell viability and apoptosis were determined using Cell Counting Kit-8, flow cytometry, Caspase-3 Assay Kit, and western blot assays. The association between let-7f-5p and circ_0085289/SOCS6 was validated via dual-luciferase reporter, RNA pull-down, and RIP assays. Circ_0085289 and SOCS6 levels were reduced, and let-7f-5p level was increased in periodontitis patients and LPS-treated PDLCs. LPS stimulation caused PDLC injury and circ_0085289 downregulation. Moreover, circ_0085289 upregulation or let-7f-5p downregulation diminished LPS-triggered PDLC injury. Besides, circ_0085289 promoted SOCS6 expression by absorbing let-7f-5p. Circ_0085289 alleviated LPS-stimulated PDLC injury via targeting let-7f-5p. Moreover, let-7f-5p targeted SOCS6 to affect LPS-resulted PDLC injury. Circ_0085289 alleviated PDLC injury induced by LPS stimulation via modulating let-7f-5p/SOCS6 axis, suggesting a promising biomarker for periodontitis treatment.
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Wenwen Du was in charge of the design of study and collection of data. Wenwen Du analyzed the data and wrote the manuscript. Li Wang and Zhen Liao designed and guided the whole study. Juan Wang instructed the study. Li Wang and Juan Wang polished the manuscript and language revision. Li Wang collected the data. All authors read and approved the final manuscript.
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Du, W., Wang, L., Liao, Z. et al. Circ_0085289 Alleviates the Progression of Periodontitis by Regulating let-7f-5p/SOCS6 Pathway. Inflammation 44, 1607–1619 (2021). https://doi.org/10.1007/s10753-021-01445-8
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DOI: https://doi.org/10.1007/s10753-021-01445-8