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Diosgenin Inhibits Excessive Proliferation and Inflammatory Response of Synovial Fibroblasts in Rheumatoid Arthritis by Targeting PDE3B

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Abstract

Rheumatoid arthritis (RA) is a chronic inflammation that can lead to loss of range of joint abnormalities in severe cases. Diosgenin has anti-inflammatory effects. This paper discussed the effect and mechanism of diosgenin on excessive proliferation and inflammatory response of synovial cells in RA. CCK-8 detected the cell viability, TUNEL assay detected the apoptosis of cells and western blot detected the expression of apoptosis-related proteins. Wound healing was used to detect cell migration and western blot detected the expression of migration-related proteins. ELISA kits were used to detect the levels of inflammatory cytokines in cells. Diosgenin can inhibit the proliferation and migration of RA synovial cells. At the same time, diosgenin could reduce the inflammatory response of RA synovial cells, during which the expression of PDE3B was significantly decreased. By overexpressing PDE3B, we found that diosgenin inhibited the proliferation, migration, and inflammatory response of RA synovial cells by downregulating PDE3B. Diosgenin can inhibit excessive proliferation and inflammatory response of synovial fibroblasts by targeting PDE3B.

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Data Availability

The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.

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Funding

The Grants-in-Aid for Scientific Research (JC2019136) from the Ministry of Nantong Science and Technology.

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Authors and Affiliations

  1. Department of Diabetes and Endocrinology, Kunming Municipal Hospital of Traditional Chinese Medicine, Kunming, 650500, Yunnan, China

    Roujun Wang

  2. Department of Diabetes and Endocrinology, Affiliated Traditional Chinese Medicine Hospital of Nantong University, 41 Jianshe Road, Nantong City, 226001, Jiangsu, China

    Yumeng Sun, Xiaowen Jin & Yongjun Cao

  3. Department of Diabetes and Endocrinology, The No.1 Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, Kunming, 650021, Yunnan, China

    Weibo Wen

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  1. Roujun Wang
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  2. Yumeng Sun
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Correspondence to Yongjun Cao.

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Wang, R., Sun, Y., Jin, X. et al. Diosgenin Inhibits Excessive Proliferation and Inflammatory Response of Synovial Fibroblasts in Rheumatoid Arthritis by Targeting PDE3B. Inflammation 44, 946–955 (2021). https://doi.org/10.1007/s10753-020-01389-5

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  • DOI: https://doi.org/10.1007/s10753-020-01389-5

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