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CYTL1 Promotes the Activation of Neutrophils in a Sepsis Model

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Abstract

As a novel cytokine, cytokine-like 1 (CYTL1) is a classical secretory protein, and its potential biological function remains to be determined. In this study, we found that expression of CYTL1 was upregulated in neutrophils upon inflammatory stimuli. We demonstrated that CYTL1 enhanced phagocytosis of Escherichia coli by activated neutrophils both in vivo and in vitro through phosphorylation of protein kinase B (Akt). CYTL1-induced chemotactic activity in lipopolysaccharide (LPS) stimulated neutrophils, and the mechanism may be related to CC chemokine receptor 2 (CCR2) mediated action. CYTL1 also increased the release of reactive oxygen species (ROS) in LPS-stimulated neutrophils. These data indicate that upon inflammatory stimulation, neutrophil-derived CYTL1 may play a crucial role in the activation of neutrophils during pathogenic infections.

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Acknowledgments

We thank Professor Han Wenling from the Department of Immunology, Peking University Health Science Center for providing the CYTL1 protein.

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  1. Department of Critical Care Medicine, Peking University People’s Hospital, Beijing, China

    Haiyan Xue, Shu Li, Xiujuan Zhao, Fuzheng Guo, Lilei Jiang, Yaxin Wang & Fengxue Zhu

  2. Trauma Medicine Center, Peking University People’s Hospital, Beijing, China

    Fengxue Zhu

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  1. Haiyan Xue
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Correspondence to Fengxue Zhu.

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The animal experimental procedures were approved by the Ethics Committee of the Peking University People’s Hospital.

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Xue, H., Li, S., Zhao, X. et al. CYTL1 Promotes the Activation of Neutrophils in a Sepsis Model. Inflammation 43, 274–285 (2020). https://doi.org/10.1007/s10753-019-01116-9

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