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Umbilical Cord Blood Mesenchymal Stem Cells Enhance Lipopolysaccharide-Induced IL-10 and IL-37 Production in THP-1 Cells

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Abstract

Umbilical cord blood mesenchymal stem cells (UCB-MSCs) have been shown to be a source of stem cells for use in cellular therapies and have immunomodulatory effects on several immune cells in an inflammatory environment. However, whether UCB-MSCs have immunomodulatory effects against lipopolysaccharide (LPS)-induced inflammatory cytokine secretion in macrophages and whether it is involved in phosphoinositide 3-kinase/protein kinase B (PI3K/Akt) signaling pathway remain unclear. After co-culture of UCB-MSCs and phorbol 12-myristate 13-acetate (PMA)-activated human THP-1 cells using a transwell system, it showed that LPS significantly induced increases in the expression levels of interleukin 10 (IL-10), interleukin 37 (IL-37), phospho-PI3K (p-PI3K), and phospho-Akt (p-Akt) in macrophages. UCB-MSCs upregulated the expression of IL-10, IL-37, p-PI3K, and p-Akt, while it had no obvious effect on PI3K and Akt levels. Inhibitors of PI3K (LY294002) significantly suppressed the expression of IL-10, IL-37, p-PI3K, and p-Akt; however, it had no effect on the expression levels of PI3K and Akt. The present study demonstrated that UCB-MSCs increased the LPS-stimulated expression of IL-10 and IL-37 in macrophages through the PI3K/Akt signaling pathway.

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Abbreviations

UCB-MSCs:

Umbilical cord blood mesenchymal stem cells

PI3K/Akt:

Hosphoinositide 3-kinase/protein kinase B

LPS:

Lipopolysaccharide

IL-10:

Interleukin 10

IL-37:

Interleukin 37

PMA:

Phorbol 12-myristate 13-acetate

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Acknowledgments

We would like to thank Jin Yang, Wenxue Liang, Yongjuan Liu, Juan Huo, and Ting Zhang for technical assistance and thank Boya Life Stem Cell Technology and the Key Laboratory of Systematic Bioengineering, Ministry of Education, Tianjin University for the provided support.

Funding

This study was supported by The National Natural Science Foundation of China (Grant No. 81300052), the Jiangsu Provincial Commission of Health and Family Planning (Grant No. H201558), and Postdoctoral Research Foundation of China (Grant No. 2015 M570420).

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  1. Ting Zhou and Yan Sun contributed equally to this work.

Authors and Affiliations

  1. Department of Emergency Medicine, Xuzhou Medical University Affiliated Hospital of Lianyungang, No.182 North Tongguan Road, Lianyungang, 222002, China

    Ting Zhou, Yan Sun, Yanli Wang, Xiaobing Chen, Luo Zhuo, Suo Xu, Jiayan Han & Xiaomin Li

  2. Department of Critical Care Medicine, Xuzhou Medical University Affiliated Hospital, No.99 West Huaihai Road, Xuzhou, 221000, China

    Lin Bu

  3. Department of Respiratory Medicine, Xuzhou Medical University Affiliated Hospital of Lianyungang, No.182 North Tongguan Road, Lianyungang, 222002, China

    Jiaxin Shi

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Zhou, T., Sun, Y., Wang, Y. et al. Umbilical Cord Blood Mesenchymal Stem Cells Enhance Lipopolysaccharide-Induced IL-10 and IL-37 Production in THP-1 Cells. Inflammation 42, 987–993 (2019). https://doi.org/10.1007/s10753-019-00960-z

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