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Protective and Therapeutic Effects of Engeletin on LPS-Induced Acute Lung Injury

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Abstract

Acute lung injury (ALI) is a serious disease with morbidity and mortality in patients. Engeletin (dihydrokaempferol 3-rhamnoside) is a flavanonol glycoside. It can be found in the skin of white grapes and white wine and is widely distributed in southeast Asia. In our study, we evaluated the protective and therapeutic effects of engeletin on lipopolysaccharide (LPS)-induced ALI in animal model. We determined the level of peroxisome proliferator-activated receptor-γ (PPAR-γ), nuclear factor kappaB (NF-κB), and IκBα by western blotting. The myeloperoxidase (MPO) activity and lung wet/dry (W/D) ratio in lung tissues were also detected. Histopathological changes and the pro-inflammatory cytokines TNF-α, IL-6, and IL-1β were determined by H&E staining and ELISA. The MPO activity and lung W/D ratio induced by LPS were attenuated by engeletin. The numbers of inflammatory cells and the levels of inflammatory cytokines in bronchoalveolar lavage fluid (BALF) were ameliorated by engeletin. Furthermore, the results also showed that engeletin significantly suppressed LPS-induced NF-κB activation. The expression of PPAR-γ was upregulated by treatment of engeletin. In conclusion, we found that engeletin had protective and therapeutic effects against LPS-induced ALI by activating PPAR-γ. Engeletin is a potentially effective agent for the treatment of lung injury.

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Funding

This study was supported by grants from the Joint Research Fund for the Department of Science and Technology of Sichuan province and the Southwest Medical University (14JC0181); and the Youth Fund of Southwest Medical University (2014QN-105; 2014QN-099).

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Authors and Affiliations

  1. Department of Anesthesiology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China

    Xian Jiang, Xiaobin Wang & Jicheng Wei

  2. Department of Pharmacology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China

    Lijuan Chen

  3. Department of Pharmacology of Pharmacy College of Southwest Medical University, Luzhou, Sichuan, China

    Zhuo Zhang & Yuhong Sun

Authors
  1. Xian Jiang
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  2. Lijuan Chen
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  3. Zhuo Zhang
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  4. Yuhong Sun
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  5. Xiaobin Wang
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  6. Jicheng Wei
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Correspondence to Jicheng Wei.

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Jiang, X., Chen, L., Zhang, Z. et al. Protective and Therapeutic Effects of Engeletin on LPS-Induced Acute Lung Injury. Inflammation 41, 1259–1265 (2018). https://doi.org/10.1007/s10753-018-0773-z

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  • DOI: https://doi.org/10.1007/s10753-018-0773-z

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